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Literature summary extracted from

  • Ilcu, L.; Roether, W.; Birke, J.; Brausemann, A.; Einsle, O.; Jendrossek, D.
    Structural and functional analysis of Latex clearing protein (Lcp) provides insight into the enzymatic cleavage of rubber (2017), Sci. Rep., 7, 6179 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.87 expression in Escherichia coli Streptomyces sp. K30

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.87 sitting-drop vapour diffusion method Streptomyces sp. K30

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.87 K167A specific activity of polyisoprene oxidation is reduced to 20%, the product spectrum is unchanged Streptomyces sp. K30
1.13.11.87 K167H specific activity of polyisoprene oxidation is reduced to 12%, the product spectrum is unchanged Streptomyces sp. K30
1.13.11.87 R164A mutant enzyme contains the heme cofactor, but shows no detectable activity Streptomyces sp. K30
1.13.11.87 T168A mutant enzyme contains the heme cofactor, but shows only 2% residual activity compared to the wild type Streptomyces sp. K30

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.87 Streptomyces sp. K30 Q3L8N0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.87
-
 Streptomyces sp. K30

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.87 cis-1,4-polyisoprene + O2 the enzyme acts as an endo-type dioxygenase producing C20 and higher oligo-isoprenoids that differ in the number of isoprene units but have the same terminal functions, CHO-CH2- and -CH2-COCH3 Streptomyces sp. K30 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.87 latex clearing protein
-
 Streptomyces sp. K30
1.13.11.87 Lcp
-
 Streptomyces sp. K30
1.13.11.87 rubber oxygenase
-
 Streptomyces sp. K30

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.87 22
-
 assay at Streptomyces sp. K30

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.87 7
-
 assay at Streptomyces sp. K30

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.87 cytochrome b-type cytochrome. The haem group of LcpK30 is ligated to the polypeptide by a proximal histidine (His198) and by a lysine residue (Lys167) as the distal axial ligand Streptomyces sp. K30