Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Maranha, A.; Moynihan, P.J.; Miranda, V.; Correia Lourenco, E.; Nunes-Costa, D.; Fraga, J.S.; Jose Barbosa Pereira, P.; Macedo-Ribeiro, S.; Ventura, M.R.; Clarke, A.J.; Empadinhas, N.
    Octanoylation of early intermediates of mycobacterial methylglucose lipopolysaccharides (2015), Sci. Rep., 5, 13610 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.273 expression in Escherichia coli as a His-tagged enzyme Mycobacterium tuberculosis
2.3.1.273 expression in Escherichia coli as a His-tagged enzyme Mycolicibacterium smegmatis
2.3.1.273 expression in Escherichia coli as a His-tagged enzyme Mycolicibacterium hassiacum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.273 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate 0.4 mM, about 20% inhibition Mycolicibacterium hassiacum
2.3.1.273 Co2+
-
 Mycolicibacterium hassiacum
2.3.1.273 EDTA
-
 Mycobacterium tuberculosis
2.3.1.273 EDTA 10 mM, about 25% loss of activity Mycolicibacterium hassiacum
2.3.1.273 EDTA
-
 Mycolicibacterium smegmatis
2.3.1.273 hexanoyl-CoA 0.12 mM, about 50% inhibition Mycolicibacterium hassiacum
2.3.1.273 Mg2+
-
 Mycolicibacterium hassiacum
2.3.1.273 octanoyl-CoA 0.16 mM, about 60% inhibition Mycolicibacterium hassiacum
2.3.1.273 octanoyl-CoA 0.16 mM, about 60% inhibition Mycolicibacterium smegmatis
2.3.1.273 Zn2+
-
 Mycolicibacterium hassiacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.273 0.01
-
 octanoyl-CoA 37°C, pH 8.0, cosubstrate: 20 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate Mycolicibacterium hassiacum
2.3.1.273 0.01
-
 hexanoyl-CoA 37°C, pH 8.0, cosubstrate: 20 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate Mycolicibacterium hassiacum
2.3.1.273 0.02
-
 hexanoyl-CoA 37°C, pH 8.0, cosubstrate: 20 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate Mycolicibacterium hassiacum
2.3.1.273 0.02
-
 octanoyl-CoA 37°C, pH 8.0, cosubstrate: 45 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate Mycolicibacterium smegmatis
2.3.1.273 0.03
-
 octanoyl-CoA 37°C, pH 8.0, cosubstrate: 45 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate Mycolicibacterium smegmatis
2.3.1.273 0.06
-
 octanoyl-CoA 37°C, pH 8.0, cosubstrate: 20 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate Mycolicibacterium hassiacum
2.3.1.273 6.6
-
 2-O-(alpha-D-glucopyranosyl)-D-glycerate 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA Mycolicibacterium hassiacum
2.3.1.273 9.5
-
 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA Mycolicibacterium hassiacum
2.3.1.273 9.8
-
 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate 37°C, pH 8.0, cosubstrate: 0.1 mM hexanoyl-CoA Mycolicibacterium hassiacum
2.3.1.273 19
-
 2-O-(alpha-D-glucopyranosyl)-D-glycerate 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA Mycolicibacterium smegmatis
2.3.1.273 20
-
 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.273 111100
-
 gel filtration, tetramer Mycobacterium tuberculosis
2.3.1.273 125200
-
 gel filtration, tetramer Mycolicibacterium hassiacum
2.3.1.273 125500
-
 gel filtration, tetramer Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.273 octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate Mycobacterium tuberculosis the enzyme is involved in the biosynthesis of methylglucose lipopolysaccharide 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
-
 ?
2.3.1.273 octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate Mycolicibacterium smegmatis the enzyme is involved in the biosynthesis of methylglucose lipopolysaccharide 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
-
 ?
2.3.1.273 octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate Mycolicibacterium hassiacum the enzyme is involved in the biosynthesis of methylglucose lipopolysaccharide 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.273 Mycobacterium tuberculosis P71725
-
-
2.3.1.273 Mycolicibacterium hassiacum K5BJH8
-
-
2.3.1.273 Mycolicibacterium smegmatis A0R109
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.273
-
 Mycobacterium tuberculosis
2.3.1.273
-
 Mycolicibacterium smegmatis
2.3.1.273
-
 Mycolicibacterium hassiacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.273 hexanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
-
 Mycobacterium tuberculosis 2-O-[6-O-hexanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
-
 ?
2.3.1.273 hexanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
-
 Mycolicibacterium hassiacum 2-O-[6-O-hexanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
-
 ?
2.3.1.273 octanoyl-CoA + 2-O-(alpha-D-glucopyranosyl)-D-glycerate lower activity than with 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate Mycolicibacterium smegmatis ?
-
 ?
2.3.1.273 octanoyl-CoA + 2-O-(alpha-D-glucopyranosyl)-D-glycerate lower activity than with 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate Mycolicibacterium hassiacum ?
-
 ?
2.3.1.273 octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
-
 Mycobacterium tuberculosis 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
-
 ?
2.3.1.273 octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
-
 Mycolicibacterium smegmatis 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
-
 ?
2.3.1.273 octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
-
 Mycolicibacterium hassiacum 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
-
 ?
2.3.1.273 octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate the enzyme is involved in the biosynthesis of methylglucose lipopolysaccharide Mycobacterium tuberculosis 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
-
 ?
2.3.1.273 octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate the enzyme is involved in the biosynthesis of methylglucose lipopolysaccharide Mycolicibacterium smegmatis 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
-
 ?
2.3.1.273 octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate the enzyme is involved in the biosynthesis of methylglucose lipopolysaccharide Mycolicibacterium hassiacum 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.273 tetramer 4 *, SDS-PAGE Mycobacterium tuberculosis
2.3.1.273 tetramer 4 *, SDS-PAGE Mycolicibacterium smegmatis
2.3.1.273 tetramer 4 *, SDS-PAGE Mycolicibacterium hassiacum

Synonyms

EC Number Synonyms Comment Organism
2.3.1.273 DGG octanoyltransferase
-
 Mycobacterium tuberculosis
2.3.1.273 DGG octanoyltransferase
-
 Mycolicibacterium smegmatis
2.3.1.273 DGG octanoyltransferase
-
 Mycolicibacterium hassiacum
2.3.1.273 octT
-
 Mycobacterium tuberculosis
2.3.1.273 octT
-
 Mycolicibacterium smegmatis
2.3.1.273 octT
-
 Mycolicibacterium hassiacum
2.3.1.273 Rv2418c locus name Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.273 45
-
-
 Mycolicibacterium hassiacum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.273 30 50 30°C: about 40% of maximal activity, 50°C: about 50% of maximal activity Mycolicibacterium hassiacum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.273 0.00017
-
 octanoyl-CoA 37°C, pH 8.0, cosubstrate: 20 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate Mycolicibacterium hassiacum
2.3.1.273 0.00017
-
 hexanoyl-CoA 37°C, pH 8.0, cosubstrate: 20 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate Mycolicibacterium hassiacum
2.3.1.273 0.00033
-
 2-O-(alpha-D-glucopyranosyl)-D-glycerate 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA Mycolicibacterium smegmatis
2.3.1.273 0.00033
-
 2-O-(alpha-D-glucopyranosyl)-D-glycerate 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA Mycolicibacterium hassiacum
2.3.1.273 0.00033
-
 octanoyl-CoA 37°C, pH 8.0, cosubstrate: 45 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate Mycolicibacterium smegmatis
2.3.1.273 0.0005
-
 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate 37°C, pH 8.0, cosubstrate: 0.1 mM hexanoyl-CoA Mycolicibacterium hassiacum
2.3.1.273 0.00067
-
 hexanoyl-CoA 37°C, pH 8.0, cosubstrate: 20 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate Mycolicibacterium hassiacum
2.3.1.273 0.0017
-
 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA Mycolicibacterium hassiacum
2.3.1.273 0.002
-
 octanoyl-CoA 37°C, pH 8.0, cosubstrate: 20 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate Mycolicibacterium hassiacum
2.3.1.273 0.037
-
 octanoyl-CoA 37°C, pH 8.0, cosubstrate: 45 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate Mycolicibacterium smegmatis
2.3.1.273 0.038
-
 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.273 7 8.5 at 37°C Mycolicibacterium smegmatis
2.3.1.273 7 8.5 at 37°C Mycolicibacterium hassiacum

General Information

EC Number General Information Comment Organism
2.3.1.273 metabolism the enzyme is essential for gowth of Mycobacterium tuberculosis. It is involved in the biosynthesis of methylglucose lipopolysaccharide Mycobacterium tuberculosis
2.3.1.273 metabolism the enzyme is essential for gowth of Mycobacterium tuberculosis. It is involved in the biosynthesis of methylglucose lipopolysaccharide Mycolicibacterium smegmatis
2.3.1.273 metabolism the enzyme is essential for gowth of Mycobacterium tuberculosis. It is involved in the biosynthesis of methylglucose lipopolysaccharide Mycolicibacterium hassiacum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.273 0.00005
-
 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate 37°C, pH 8.0, cosubstrate: 0.1 mM hexanoyl-CoA Mycolicibacterium hassiacum
2.3.1.273 0.00005
-
 2-O-(alpha-D-glucopyranosyl)-D-glycerate 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA Mycolicibacterium hassiacum
2.3.1.273 0.00017
-
 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA Mycolicibacterium hassiacum
2.3.1.273 0.0018
-
 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA Mycolicibacterium smegmatis
2.3.1.273 0.002
-
 2-O-(alpha-D-glucopyranosyl)-D-glycerate 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA Mycolicibacterium smegmatis
2.3.1.273 0.018
-
 octanoyl-CoA 37°C, pH 8.0, cosubstrate: 20 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate Mycolicibacterium hassiacum
2.3.1.273 0.022
-
 hexanoyl-CoA 37°C, pH 8.0, cosubstrate: 20 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate Mycolicibacterium hassiacum
2.3.1.273 0.025
-
 octanoyl-CoA 37°C, pH 8.0, cosubstrate: 45 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate Mycolicibacterium smegmatis
2.3.1.273 0.032
-
 octanoyl-CoA 37°C, pH 8.0, cosubstrate: 20 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate Mycolicibacterium hassiacum
2.3.1.273 0.035
-
 hexanoyl-CoA 37°C, pH 8.0, cosubstrate: 20 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate Mycolicibacterium hassiacum
2.3.1.273 1.43
-
 octanoyl-CoA 37°C, pH 8.0, cosubstrate: 45 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate Mycolicibacterium smegmatis