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Literature summary extracted from

  • Deryusheva, S.; Gall, J.G.
    Dual nature of pseudouridylation in U2 snRNA Pus1p-dependent and Pus1p-independent activities in yeasts and higher eukaryotes (2017), RNA, 23, 1060-1067 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.B22 additional information generation of gene deletion mutant DELTApus1. which shows a U2 snRNA modification pattern identical to its parental wild-type strain ED666 Schizosaccharomyces pombe
5.4.99.B22 additional information generation of Pus1 knockout mice Mus musculus
5.4.99.B22 additional information SNORA71 is expressed in an enzyme knockout mutant yeast strain, but do not restore pseudouridylation of the endogenous yeast U2 snRNA Xenopus tropicalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.B22 additional information Saccharomyces cerevisiae Pus1p is the only pseudouridine synthase in Saccharomyces cerevisiae that is active on U2 snRNA at position 44. Yeast U2 snRNA is normally pseudouridylated at positions 35, 42, and 44 ?
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?
5.4.99.B22 additional information Saccharomyces cerevisiae BY4741 Pus1p is the only pseudouridine synthase in Saccharomyces cerevisiae that is active on U2 snRNA at position 44. Yeast U2 snRNA is normally pseudouridylated at positions 35, 42, and 44 ?
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?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.B22 Caenorhabditis elegans
-
-
-
5.4.99.B22 Drosophila melanogaster
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-
-
5.4.99.B22 Drosophila melanogaster yw
-
-
-
5.4.99.B22 Mus musculus
-
-
-
5.4.99.B22 Saccharomyces cerevisiae
-
-
-
5.4.99.B22 Saccharomyces cerevisiae BY4741
-
-
-
5.4.99.B22 Schizosaccharomyces pombe
-
-
-
5.4.99.B22 Schizosaccharomyces pombe 972
-
-
-
5.4.99.B22 Xenopus tropicalis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.B22 additional information Pus1p is the only pseudouridine synthase in Saccharomyces cerevisiae that is active on U2 snRNA at position 44. Yeast U2 snRNA is normally pseudouridylated at positions 35, 42, and 44 Saccharomyces cerevisiae ?
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?
5.4.99.B22 additional information dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Position 44 in yeast U2 snRNA (equivalent to position 43 in vertebrates) is a genuine substrate for Pus1p. Besides U2 and U6 snRNAs in Saccharomyces cerevisiae, the enzyme modifies uridines in many different tRNAs. Substrate specificity analysis and comparison of different species, two types of pseudouridine synthase that catalyze the formation of U2-?43 (or 44) in yeasts, Drosophila, and vertebrates Saccharomyces cerevisiae ?
-
?
5.4.99.B22 additional information dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Position 44 in yeast U2 snRNA (equivalent to position 43 in vertebrates) is a genuine substrate for Pus1p. In vertrbrates, SCARNA8 (also known as U92 scaRNA) is a guide for U2-Psi43 in addition to established targets U2-Psi34/Psi44. Substrate specificity analysis and comparison of different species, two types of pseudouridine synthase that catalyze the formation of U2-?43 (or 44) in yeasts, Drosophila, and vertebrates Xenopus tropicalis ?
-
?
5.4.99.B22 additional information dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Position 44 in yeast U2 snRNA (equivalent to position 43 in vertebrates) is a genuine substrate for Pus1p. In vertrbrates, SCARNA8 (also known as U92 scaRNA) is a guide for U2-Psi43 in addition to established targets U2-Psi34/Psi44. U2 snRNA is identified as a genuine substrate for mPus1p. Substrate specificity analysis and comparison of different species, two types of pseudouridine synthase that catalyze the formation of U2-Psi43 (or 44) in yeasts, Drosophila, and vertebrates Mus musculus ?
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?
5.4.99.B22 additional information dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Position 44 in yeast U2 snRNA (equivalent to position 43 in vertebrates) is a genuine substrate for Pus1p. Substrate specificity analysis and comparison of different species, two types of pseudouridine synthase that catalyze the formation of U2-?43 (or 44) in yeasts, Drosophila, and vertebrates Drosophila melanogaster ?
-
?
5.4.99.B22 additional information dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Position 44 in yeast U2 snRNA (equivalent to position 43 in vertebrates) is a genuine substrate for Pus1p. Substrate specificity analysis and comparison of different species, two types of pseudouridine synthase that catalyze the formation of U2-Psi43 (or 44) in yeasts, Drosophila, and vertebrates Schizosaccharomyces pombe ?
-
?
5.4.99.B22 additional information dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Pus1p from Caenorhabditis elegans has no enzymatic activity on U2 snRNA when expressed in yeast cells. Substrate specificity analysis and comparison of different species Caenorhabditis elegans ?
-
?
5.4.99.B22 additional information Pus1p is the only pseudouridine synthase in Saccharomyces cerevisiae that is active on U2 snRNA at position 44. Yeast U2 snRNA is normally pseudouridylated at positions 35, 42, and 44 Saccharomyces cerevisiae BY4741 ?
-
?
5.4.99.B22 additional information dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Position 44 in yeast U2 snRNA (equivalent to position 43 in vertebrates) is a genuine substrate for Pus1p. Besides U2 and U6 snRNAs in Saccharomyces cerevisiae, the enzyme modifies uridines in many different tRNAs. Substrate specificity analysis and comparison of different species, two types of pseudouridine synthase that catalyze the formation of U2-?43 (or 44) in yeasts, Drosophila, and vertebrates Saccharomyces cerevisiae BY4741 ?
-
?
5.4.99.B22 additional information dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Position 44 in yeast U2 snRNA (equivalent to position 43 in vertebrates) is a genuine substrate for Pus1p. Substrate specificity analysis and comparison of different species, two types of pseudouridine synthase that catalyze the formation of U2-?43 (or 44) in yeasts, Drosophila, and vertebrates Drosophila melanogaster yw ?
-
?
5.4.99.B22 additional information dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Position 44 in yeast U2 snRNA (equivalent to position 43 in vertebrates) is a genuine substrate for Pus1p. Substrate specificity analysis and comparison of different species, two types of pseudouridine synthase that catalyze the formation of U2-Psi43 (or 44) in yeasts, Drosophila, and vertebrates Schizosaccharomyces pombe 972 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.B22 cePus1p
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Caenorhabditis elegans
5.4.99.B22 dmPus1p
-
Drosophila melanogaster
5.4.99.B22 mPus1p
-
Mus musculus
5.4.99.B22 Pus1p
-
Drosophila melanogaster
5.4.99.B22 Pus1p
-
Mus musculus
5.4.99.B22 Pus1p
-
Saccharomyces cerevisiae
5.4.99.B22 Pus1p
-
Schizosaccharomyces pombe
5.4.99.B22 Pus1p
-
Caenorhabditis elegans
5.4.99.B22 Pus1p
-
Xenopus tropicalis
5.4.99.B22 spPus1p
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Schizosaccharomyces pombe
5.4.99.B22 xtPus1p
-
Xenopus tropicalis

General Information

EC Number General Information Comment Organism
5.4.99.B22 evolution Pus1p is a member of the TruA pseudouridine synthase family Drosophila melanogaster
5.4.99.B22 evolution Pus1p is a member of the TruA pseudouridine synthase family Mus musculus
5.4.99.B22 evolution Pus1p is a member of the TruA pseudouridine synthase family Saccharomyces cerevisiae
5.4.99.B22 evolution Pus1p is a member of the TruA pseudouridine synthase family Schizosaccharomyces pombe
5.4.99.B22 evolution Pus1p is a member of the TruA pseudouridine synthase family Caenorhabditis elegans
5.4.99.B22 evolution Pus1p is a member of the TruA pseudouridine synthase family Xenopus tropicalis
5.4.99.B22 malfunction in mutant worms, the absence of Pus1p activity on tRNAs has no effect on U2 snRNA modification Caenorhabditis elegans
5.4.99.B22 malfunction U2 snRNAs from Pus1 knockout mice show no changes in their modification patterns when compared to wild-type U2 snRNAs. In Pus1-knockout mice, Pus1p-dependent modification of tRNAs is missing. No differences between wild-type and mutant mice in their U2 snRNA modification patterns Mus musculus
5.4.99.B22 malfunction U2 snRNAs from pus1DELTA Schizosaccharomyces pombe knockout strain show no changes in their modification patterns when compared to wild-type U2 snRNAs. A novel box H/ACA RNA is found encoded downstream from the RPC10 gene and experimentally verified its guide RNA activity for positioning Psi43 and Psi44 in U2 snRNA Schizosaccharomyces pombe
5.4.99.B22 additional information screening for other potential guide RNAs for pseudouridylation of position 43 in U2 snRNA, vertebrate box H/ACA RNAs for antisense elements specific to the U2 snRNA branch point recognition region. The SNORA71 5'-terminal pseudouridylation pocket can base pair with U2 snRNA, both positions 43 and 41 can be modified by base-pairing within the same pseudouridylation pocket Mus musculus
5.4.99.B22 additional information screening for other potential guide RNAs for pseudouridylation of position 43 in U2 snRNA, vertebrate box H/ACA RNAs for antisense elements specific to the U2 snRNA branch point recognition region. The SNORA71 5'-terminal pseudouridylation pocket can base pair with U2 snRNA, both positions 43 and 41 can be modified by base-pairing within the same pseudouridylation pocket Xenopus tropicalis
5.4.99.B22 additional information U2 snRNA can be modified in Caenorhabditis elegans by a Pus1p-independent mechanism Caenorhabditis elegans
5.4.99.B22 additional information U2 snRNA can be modified in Schizosaccharomyces pombe by a Pus1p-independent mechanism Schizosaccharomyces pombe
5.4.99.B22 physiological function in vertebrate SCARNA8, stronger binding in the alternative configuration to position U2-Psi45 might facilitate efficient sliding to position U2-Psi44. Multiple guide RNAs are typically assigned to the most important modified positions. Pseudouridines at the branch point recognition region of U2 snRNA represent such functionally crucial modifications Mus musculus
5.4.99.B22 physiological function in vertebrate SCARNA8, stronger binding in the alternative configuration to position U2-Psi45 might facilitate efficient sliding to position U2-Psi44. Multiple guide RNAs are typically assigned to the most important modified positions. Pseudouridines at the branch point recognition region of U2 snRNA represent such functionally crucial modifications Xenopus tropicalis
5.4.99.B22 physiological function multiple guide RNAs are typically assigned to the most important modified positions. Pseudouridines at the branch point recognition region of U2 snRNA represent such functionally crucial modifications Drosophila melanogaster
5.4.99.B22 physiological function multiple guide RNAs are typically assigned to the most important modified positions. Pseudouridines at the branch point recognition region of U2 snRNA represent such functionally crucial modifications Saccharomyces cerevisiae
5.4.99.B22 physiological function multiple guide RNAs are typically assigned to the most important modified positions. Pseudouridines at the branch point recognition region of U2 snRNA represent such functionally crucial modifications Schizosaccharomyces pombe
5.4.99.B22 physiological function multiple guide RNAs are typically assigned to the most important modified positions. Pseudouridines at the branch point recognition region of U2 snRNA represent such functionally crucial modifications Caenorhabditis elegans