EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.41 | expressed in Escherichia coli DH5alpha cells | Thermotoga maritima |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.1.41 | crystal structure of the P228A variant is determined at 2.1 A resolution. The replacement does not affect the overall secondary, tertiary, and quaternary structures and moderately decreases the thermal stability | Thermotoga maritima |
3.1.1.41 | mutant enzyme P228A, hanging drop vapor diffusion method, using 0.05M ammonium sulfate, 0.1 M Bis-Tris pH 6.5 and 30% (v/v) pentaerythritolethoxylate | Thermotoga maritima |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.1.41 | additional information | construction of truncation variants lacking four and ten residues of the N-terminal region. The truncations do not affect the secondary structure or the fold but modulate the oligomerization dynamics. A modest in substrate specificity for acetylated xylose is observed compared with acetylated glucose. Variants show a drastic reduction of 30-40°C in the optimum temperature for activity | Thermotoga maritima |
3.1.1.41 | P228A | the mutation does not affect the overall secondary, tertiary, and quaternary structures and moderately decreases the thermal stability. However, the wild type cis conformation of the 227-228 peptide bond adopts a trans conformation in the variant. The results suggest that the conserved proline residue is responsible for the cis conformation of the peptide and shapes the geometry of the active site. Elimination of the pyrrolidine ring results in the loss of van der Waals and hydrophobic interactions with both the alcohol and acyl moeities of the ester substrate, leading to significant impairment of the activity and perturbation of substrate specificity | Thermotoga maritima |
3.1.1.41 | P228A | the mutation does not affect the overall secondary, tertiary, and quaternary structures, but has a significantly decreasing effect on the activity and moderately decreases the thermal stability of the enzyme. The wild type cis conformation of the 227-228 peptide bond adopts a trans conformation in the variant | Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.41 | 0.059 | - |
xylose tetraacetate | mutant lacking 10 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 0.134 | - |
xylose tetraacetate | mutant lacking 4 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 0.134 | - |
xylose tetraacetate | wild-type including expression and purification tag, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 0.148 | - |
4-methylumbelliferyl acetate | wild-type including expression and purification tag, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 0.175 | - |
4-methylumbelliferyl acetate | mutant lacking 4 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 0.175 | - |
4-nitrophenyl acetate | wild-type including expression and purification tag, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 0.187 | - |
4-methylumbelliferyl acetate | mutant lacking 10 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 0.189 | - |
xylose tetraacetate | wild-type, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 0.193 | - |
4-nitrophenyl acetate | wild-type, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 0.197 | - |
4-methylumbelliferyl acetate | wild-type, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 0.203 | - |
Glucose pentaacetate | mutant lacking 4 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 0.207 | - |
Glucose pentaacetate | mutant lacking 10 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 0.207 | - |
Glucose pentaacetate | wild-type including expression and purification tag, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 0.224 | - |
Glucose pentaacetate | wild-type, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 0.231 | - |
4-nitrophenyl acetate | mutant lacking 4 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 0.309 | - |
4-nitrophenyl acetate | mutant lacking 10 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 1.12 | - |
7-aminocephalosporanic acid | mutant lacking 4 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 1.135 | - |
7-aminocephalosporanic acid | wild-type, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 1.384 | - |
7-aminocephalosporanic acid | wild-type including expression and purification tag, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 1.563 | - |
7-aminocephalosporanic acid | mutant lacking 10 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.41 | 76000 | 78000 | sedimentation equilibrium centrifugation, minor peak | Thermotoga maritima |
3.1.1.41 | 210000 | 220000 | sedimentation equilibrium centrifugation, dominant peak | Thermotoga maritima |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.41 | Thermotoga maritima | Q9WXT2 | - |
- |
3.1.1.41 | Thermotoga maritima ATCC 43589 | Q9WXT2 | - |
- |
3.1.1.41 | Thermotoga maritima DSM 3109 | Q9WXT2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.41 | - |
Thermotoga maritima |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.41 | 0.075 | - |
substrate: 7-aminocephalosporanic acid, pH 8.0, 50°C, mutant enzyme P228A | Thermotoga maritima |
3.1.1.41 | 0.075 | - |
with 7-amino-cephalosporanic acid as substrate, mutant enzyme P228A, at pH 8.0 and 50°C | Thermotoga maritima |
3.1.1.41 | 0.47 | - |
substrate: 1-naphthyl acetate, pH 8.0, 50°C, mutant enzyme P228A | Thermotoga maritima |
3.1.1.41 | 0.47 | - |
with 1-naphthyl acetate as substrate, mutant enzyme P228A, at pH 8.0 and 50°C | Thermotoga maritima |
3.1.1.41 | 1.78 | - |
substrate: 4-nitrophenyl acetate, pH 8.0, 50°C, mutant enzyme P228A | Thermotoga maritima |
3.1.1.41 | 1.78 | - |
with 4-nitrophenyl acetate as substrate, mutant enzyme P228A, at pH 8.0 and 50°C | Thermotoga maritima |
3.1.1.41 | 3.98 | - |
substrate: 4-methylumbelliferyl acetate, pH 8.0, 50°C, mutant enzyme P228A | Thermotoga maritima |
3.1.1.41 | 3.98 | - |
with 4-methylumbelliferyl acetate as substrate, mutant enzyme P228A, at pH 8.0 and 50°C | Thermotoga maritima |
3.1.1.41 | 10 | - |
substrate: 7-aminocephalosporanic acid, pH 8.0, 50°C, wild-type enzyme | Thermotoga maritima |
3.1.1.41 | 10 | - |
with 7-amino-cephalosporanic acid as substrate, wild type enzyme, at pH 8.0 and 50°C | Thermotoga maritima |
3.1.1.41 | 16.81 | - |
substrate: 1-naphthyl acetate, pH 8.0, 50°C, wild-type enzyme | Thermotoga maritima |
3.1.1.41 | 16.91 | - |
with 1-naphthyl acetate as substrate, wild type enzyme, at pH 8.0 and 50°C | Thermotoga maritima |
3.1.1.41 | 25.31 | - |
substrate: D-glucose pentaacetate, pH 8.0, 50°C, mutant enzyme P228A | Thermotoga maritima |
3.1.1.41 | 25.31 | - |
with D-glucose pentaacetate as substrate, mutant enzyme P228A, at pH 8.0 and 50°C | Thermotoga maritima |
3.1.1.41 | 29.8 | - |
substrate: D-xylose tetraacetate, pH 8.0, 50°C, mutant enzyme P228A | Thermotoga maritima |
3.1.1.41 | 29.8 | - |
with D-xylose tetraacetate as substrate, mutant enzyme P228A, at pH 8.0 and 50°C | Thermotoga maritima |
3.1.1.41 | 41.49 | - |
substrate: 4-methylumbelliferyl acetate, pH 8.0, 50°C, wild-type enzyme | Thermotoga maritima |
3.1.1.41 | 41.49 | - |
with 4-methylumbelliferyl acetate as substrate, wild type enzyme, at pH 8.0 and 50°C | Thermotoga maritima |
3.1.1.41 | 49.33 | - |
substrate: 4-nitrophenyl acetate, pH 8.0, 50°C, wild-type enzyme | Thermotoga maritima |
3.1.1.41 | 49.33 | - |
with 4-nitrophenyl acetate as substrate, wild type enzyme, at pH 8.0 and 50°C | Thermotoga maritima |
3.1.1.41 | 346.22 | - |
substrate: D-xylose tetraacetate, pH 8.0, 50°C, wild-type enzyme | Thermotoga maritima |
3.1.1.41 | 346.22 | - |
with D-xylose tetraacetate as substrate, wild type enzyme, at pH 8.0 and 50°C | Thermotoga maritima |
3.1.1.41 | 415.5 | - |
substrate: D-glucose pentaacetate, pH 8.0, 50°C, wild-type enzyme | Thermotoga maritima |
3.1.1.41 | 415.5 | - |
with D-glucose pentaacetate as substrate, wild type enzyme, at pH 8.0 and 50°C | Thermotoga maritima |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.41 | 1-naphthyl acetate + H2O | - |
Thermotoga maritima | 1-naphthol + acetate | - |
? | |
3.1.1.41 | 1-naphthyl acetate + H2O | - |
Thermotoga maritima DSM 3109 | 1-naphthol + acetate | - |
? | |
3.1.1.41 | 1-naphthyl acetate + H2O | - |
Thermotoga maritima ATCC 43589 | 1-naphthol + acetate | - |
? | |
3.1.1.41 | 4-methylumbelliferyl acetate + H2O | - |
Thermotoga maritima | 4-methylumbelliferol + acetate | - |
? | |
3.1.1.41 | 4-methylumbelliferyl acetate + H2O | - |
Thermotoga maritima DSM 3109 | 4-methylumbelliferol + acetate | - |
? | |
3.1.1.41 | 4-methylumbelliferyl acetate + H2O | - |
Thermotoga maritima ATCC 43589 | 4-methylumbelliferol + acetate | - |
? | |
3.1.1.41 | 4-nitrophenyl acetate + H2O | - |
Thermotoga maritima | 4-nitrophenol + acetate | - |
? | |
3.1.1.41 | 4-nitrophenyl acetate + H2O | - |
Thermotoga maritima DSM 3109 | 4-nitrophenol + acetate | - |
? | |
3.1.1.41 | 4-nitrophenyl acetate + H2O | - |
Thermotoga maritima ATCC 43589 | 4-nitrophenol + acetate | - |
? | |
3.1.1.41 | 7-amino-cephalosporanic acid + H2O | - |
Thermotoga maritima | 7-amino-3-deacetylcephalosporanic acid + acetate | - |
? | |
3.1.1.41 | 7-aminocephalosporanic acid + H2O | - |
Thermotoga maritima | acetate + deacetyl-7-aminocephalosporanic acid | - |
? | |
3.1.1.41 | 7-aminocephalosporanic acid + H2O | - |
Thermotoga maritima DSM 3109 | acetate + deacetyl-7-aminocephalosporanic acid | - |
? | |
3.1.1.41 | 7-aminocephalosporanic acid + H2O | - |
Thermotoga maritima ATCC 43589 | acetate + deacetyl-7-aminocephalosporanic acid | - |
? | |
3.1.1.41 | 7-aminocephalosporanic acid + H2O | - |
Thermotoga maritima | 7-amino-3-deacetylcephalosporanic acid + acetate | - |
? | |
3.1.1.41 | D-glucose pentaacetate + H2O | - |
Thermotoga maritima | D-glucose + acetate | - |
? | |
3.1.1.41 | D-glucose pentaacetate + H2O | - |
Thermotoga maritima DSM 3109 | D-glucose + acetate | - |
? | |
3.1.1.41 | D-glucose pentaacetate + H2O | - |
Thermotoga maritima ATCC 43589 | D-glucose + acetate | - |
? | |
3.1.1.41 | D-glucose pentaacetate + H2O | - |
Thermotoga maritima | ? | - |
? | |
3.1.1.41 | D-xylose tetraacetate + H2O | - |
Thermotoga maritima | ? | - |
? | |
3.1.1.41 | D-xylose tetraacetate + H2O | - |
Thermotoga maritima | D-xylose + acetate | - |
? | |
3.1.1.41 | fluorescein di(acetoxymethyl ether) + H2O | - |
Thermotoga maritima | ? | - |
? | |
3.1.1.41 | glucose pentaacetate + H2O | - |
Thermotoga maritima | ? + acetate | - |
? | |
3.1.1.41 | xylose tetraacetate + H2O | - |
Thermotoga maritima | ? + acetate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.41 | ACE | - |
Thermotoga maritima |
3.1.1.41 | TM0077 | - |
Thermotoga maritima |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.41 | 50 | - |
assay at | Thermotoga maritima |
3.1.1.41 | 60 | 70 | truncation variants lacking four and ten residues of the N-terminus | Thermotoga maritima |
3.1.1.41 | 95 | - |
excessive auto-hydrolysis of the substrate at higher temperatures limits the assay at 95°C | Thermotoga maritima |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.41 | 105 | - |
the unfolding transition for the wild type occurs at a temperature higher than 105°C | Thermotoga maritima |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.41 | 15.5 | - |
7-aminocephalosporanic acid | mutant lacking 10 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 20.2 | - |
7-aminocephalosporanic acid | mutant lacking 4 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 23.8 | - |
7-aminocephalosporanic acid | wild-type, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 24.8 | - |
7-aminocephalosporanic acid | wild-type including expression and purification tag, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 32.2 | - |
4-methylumbelliferyl acetate | mutant lacking 10 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 39.8 | - |
4-methylumbelliferyl acetate | mutant lacking 4 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 42.7 | - |
4-methylumbelliferyl acetate | wild-type including expression and purification tag, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 47.8 | - |
4-nitrophenyl acetate | mutant lacking 10 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 48.7 | - |
4-nitrophenyl acetate | mutant lacking 4 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 60.3 | - |
4-nitrophenyl acetate | wild-type including expression and purification tag, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 63 | - |
4-methylumbelliferyl acetate | wild-type, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 96.2 | - |
4-nitrophenyl acetate | wild-type, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 169.8 | - |
xylose tetraacetate | mutant lacking 10 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 266.7 | - |
xylose tetraacetate | mutant lacking 4 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 280.7 | - |
xylose tetraacetate | wild-type including expression and purification tag, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 293.2 | - |
Glucose pentaacetate | mutant lacking 10 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 295.5 | - |
Glucose pentaacetate | mutant lacking 4 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 323.2 | - |
Glucose pentaacetate | wild-type including expression and purification tag, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 326 | - |
xylose tetraacetate | wild-type, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 347 | - |
Glucose pentaacetate | wild-type, pH 8.0, 50°C | Thermotoga maritima |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.41 | 8 | - |
- |
Thermotoga maritima |
3.1.1.41 | 8 | - |
assay at | Thermotoga maritima |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.41 | 7.5 | 8.5 | - |
Thermotoga maritima |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.41 | 9 | - |
7-aminocephalosporanic acid | mutant lacking 10 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 18 | - |
7-aminocephalosporanic acid | mutant lacking 4 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 18 | - |
7-aminocephalosporanic acid | wild-type including expression and purification tag, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 21 | - |
7-aminocephalosporanic acid | wild-type, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 155 | - |
4-nitrophenyl acetate | mutant lacking 10 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 171 | - |
4-methylumbelliferyl acetate | mutant lacking 10 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 211 | - |
4-nitrophenyl acetate | mutant lacking 4 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 228 | - |
4-methylumbelliferyl acetate | mutant lacking 4 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 289 | - |
4-methylumbelliferyl acetate | wild-type including expression and purification tag, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 320 | - |
4-methylumbelliferyl acetate | wild-type, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 344 | - |
4-nitrophenyl acetate | wild-type including expression and purification tag, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 497 | - |
4-nitrophenyl acetate | wild-type, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 1418 | - |
Glucose pentaacetate | mutant lacking 10 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 1456 | - |
Glucose pentaacetate | mutant lacking 4 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 1555 | - |
Glucose pentaacetate | wild-type, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 1558 | - |
Glucose pentaacetate | wild-type including expression and purification tag, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 1725 | - |
xylose tetraacetate | wild-type, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 1994 | - |
xylose tetraacetate | mutant lacking 4 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 2083 | - |
xylose tetraacetate | wild-type including expression and purification tag, pH 8.0, 50°C | Thermotoga maritima | |
3.1.1.41 | 2866 | - |
xylose tetraacetate | mutant lacking 10 amino acids at N-terminus, pH 8.0, 50°C | Thermotoga maritima |