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Literature summary extracted from
- Combining ancestral sequence reconstruction with protein design to identify an interface hotspot in a key metabolic enzyme complex (2017), Proteins, 85, 312-321 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.3.2.10 | expression of HisF from Pyrobaculum arsenaticum {DSM 13514} in Escherichia coli BL21-Gold (DE3) | Zymomonas mobilis |
| 4.3.2.10 | expression of HisF from Thermotoga maritima or Pyrobaculum arsenaticum {DSM 13514} in Escherichia coli BL21-Gold (DE3) | Pyrobaculum arsenaticum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.2.10 | Pyrobaculum arsenaticum | A4WHB6 AND A4WHA5 | A4WHB6: subunit HisF, A4WHA5: subunit HisH | - |
| 4.3.2.10 | Pyrobaculum arsenaticum DSM 13514 | A4WHB6 AND A4WHA5 | A4WHB6: subunit HisF, A4WHA5: subunit HisH | - |
| 4.3.2.10 | Zymomonas mobilis | Q5NMD6 AND Q5NMD4 | Q5NMD6: subunit HisF, Q5NMD4: subunit HisH | - |
| 4.3.2.10 | Zymomonas mobilis ZM4 | Q5NMD6 AND Q5NMD4 | Q5NMD6: subunit HisF, Q5NMD4: subunit HisH | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.3.2.10 | - |
Zymomonas mobilis |
| 4.3.2.10 | - |
Pyrobaculum arsenaticum |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.3.2.10 | More | subunit HisH from Zymomonas mobilis (zmHisH) binds with high affinity to the reconstructed subunit HisF from the last universal common ancestor (LUCA-HisF) but not to subunit HisF from Pyrobaculum arsenaticum (paHisF), which differ by 103 residues. Phe74 of LUCA-HisF is decisive for its affinity for zmHisH | Zymomonas mobilis |
| 4.3.2.10 | More | subunit HisH from Zymomonas mobilis (zmHisH) binds with high affinity to the reconstructed subunit HisF from the last universal common ancestor (LUCA-HisF) but not to subunit HisF from Pyrobaculum arsenaticum (paHisF), which differ by 103 residues. Phe74 of LUCA-HisF is decisive for its affinity for zmHisH | Pyrobaculum arsenaticum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.3.2.10 | HisF:HisH complex | - |
Zymomonas mobilis |
| 4.3.2.10 | HisF:HisH complex | - |
Pyrobaculum arsenaticum |
| 4.3.2.10 | ImGP synthase | - |
Zymomonas mobilis |
| 4.3.2.10 | ImGP synthase | - |
Pyrobaculum arsenaticum |
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Release 2023.1 (January 2023)
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