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Literature summary extracted from
- Recombinant expression of a functional myo-inositol-1-phosphate synthase (MIPS) in Mycobacterium smegmatis (2015), Protein J., 34, 380-390 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.5.1.4 | expressed in Escherichia coli BL21(DE3) cells and Mycobacterium smegmatis strain mc24517 | Mycobacterium tuberculosis |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.5.1.4 | Zn2+ | required | Mycobacterium tuberculosis |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.5.1.4 | 140000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.5.1.4 | D-Glucose 6-phosphate | Mycobacterium tuberculosis | - |
L-myo-Inositol 1-phosphate | - |
? | |
| 5.5.1.4 | D-Glucose 6-phosphate | Mycobacterium tuberculosis H37Rv | - |
L-myo-Inositol 1-phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.5.1.4 | Mycobacterium tuberculosis | - |
- |
- |
| 5.5.1.4 | Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.5.1.4 | IMAC column chromatography and DEAE column chromatography | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.5.1.4 | D-Glucose 6-phosphate | - |
Mycobacterium tuberculosis | L-myo-Inositol 1-phosphate | - |
? | |
| 5.5.1.4 | D-Glucose 6-phosphate | - |
Mycobacterium tuberculosis H37Rv | L-myo-Inositol 1-phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.5.1.4 | homotrimer | 3 * 41000, Halo-tagged protein, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.5.1.4 | MIPS | - |
Mycobacterium tuberculosis |
| 5.5.1.4 | Myo-inositol-1-phosphate synthase | - |
Mycobacterium tuberculosis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.5.1.4 | NAD+ | required | Mycobacterium tuberculosis | |
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Release 2023.1 (January 2023)
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