EC Number | Application | Comment | Organism |
---|---|---|---|
4.2.1.123 | diagnostics | tetrahymanol is a polycyclic triterpenoid lipid in the Tetrahymena ciliates. The diagenetic product, gammacerane, is often used as a biomarker for water column stratification in ancient ecosystems | Tetrahymena thermophila |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.B28 | gene MEALZ_1626 or rhs, sequence comparisons and phylogenetic analysis, phylogenetics and metagenomics, recombinant expression in Methylococcus capsulatus Bath strains | Methylotuvimicrobium alcaliphilum |
4.2.1.B28 | gene Rpal_0860 or ths, sequence comparisons and phylogenetic analysis, phylogenetics and metagenomics, recombinant expression in and functional complementation of DELTAths deletion mutant strain of Methylomicrobium alcaliphilum | Rhodopseudomonas palustris |
4.2.1.123 | gene ths, DNA and amino acid sequence determination and analysis, sequence comparisons | Tetrahymena thermophila |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.B28 | additional information | construction of a DELTAths deletion mutant, functional complementation by expression of gene Rpal_0860, encoding wild-type Ths, and gene shc from Methylomicrobium alcaliphilum strain 20Z | Methylotuvimicrobium alcaliphilum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.B28 | additional information | Rhodopseudomonas palustris | the enzyme catalyzes steps in the synthesis of tetrahymanol from hopene | ? | - |
? | |
4.2.1.B28 | additional information | Methylotuvimicrobium alcaliphilum | the enzyme catalyzes steps in the synthesis of tetrahymanol from hopene | ? | - |
? | |
4.2.1.B28 | additional information | Rhodopseudomonas palustris TIE-1 | the enzyme catalyzes steps in the synthesis of tetrahymanol from hopene | ? | - |
? | |
4.2.1.B28 | additional information | Methylotuvimicrobium alcaliphilum DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z | the enzyme catalyzes steps in the synthesis of tetrahymanol from hopene | ? | - |
? | |
4.2.1.123 | squalene + H2O | Tetrahymena thermophila | - |
tetrahymanol | - |
? | |
4.2.1.123 | squalene + H2O | Tetrahymena thermophila SB210 | - |
tetrahymanol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.B28 | Methylotuvimicrobium alcaliphilum | G4SZH3 | - |
- |
4.2.1.B28 | Methylotuvimicrobium alcaliphilum DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z | G4SZH3 | - |
- |
4.2.1.B28 | Rhodopseudomonas palustris | B3QED8 | - |
- |
4.2.1.B28 | Rhodopseudomonas palustris TIE-1 | B3QED8 | - |
- |
4.2.1.123 | Tetrahymena thermophila | Q24FB1 | - |
- |
4.2.1.123 | Tetrahymena thermophila SB210 | Q24FB1 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.B28 | hopene + H2O | - |
Rhodopseudomonas palustris | tetrahymanol | - |
? | |
4.2.1.B28 | hopene + H2O | - |
Rhodopseudomonas palustris TIE-1 | tetrahymanol | - |
? | |
4.2.1.B28 | additional information | the enzyme catalyzes steps in the synthesis of tetrahymanol from hopene | Rhodopseudomonas palustris | ? | - |
? | |
4.2.1.B28 | additional information | the enzyme catalyzes steps in the synthesis of tetrahymanol from hopene | Methylotuvimicrobium alcaliphilum | ? | - |
? | |
4.2.1.B28 | additional information | the enzyme catalyzes steps in the synthesis of tetrahymanol from hopene | Rhodopseudomonas palustris TIE-1 | ? | - |
? | |
4.2.1.B28 | additional information | the enzyme catalyzes steps in the synthesis of tetrahymanol from hopene | Methylotuvimicrobium alcaliphilum DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z | ? | - |
? | |
4.2.1.123 | squalene + H2O | - |
Tetrahymena thermophila | tetrahymanol | - |
? | |
4.2.1.123 | squalene + H2O | - |
Tetrahymena thermophila SB210 | tetrahymanol | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.B28 | MEALZ_1626 | - |
Methylotuvimicrobium alcaliphilum |
4.2.1.B28 | Rpal_0860 | - |
Rhodopseudomonas palustris |
4.2.1.B28 | Ths | - |
Rhodopseudomonas palustris |
4.2.1.B28 | Ths | - |
Methylotuvimicrobium alcaliphilum |
4.2.1.123 | squalene-tetrahymanol cyclase | - |
Tetrahymena thermophila |
4.2.1.123 | STC | - |
Tetrahymena thermophila |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.1.B28 | evolution | tetrahymanol synthase (Ths) is found in a variety of bacterial genomes, including aerobic methanotrophs, nitrite-oxidizers, and sulfate-reducers, and in a subset of aquatic and terrestrial metagenomes. The potential to produce tetrahymanol is more widespread in the bacterial domain than previously thought. Ths is not encoded in any eukaryotic genomes, nor is it homologous to eukaryotic squalene-tetrahymanol cyclase (EC 4.2.1.123), which catalyzes the cyclization of squalene directly to tetrahymanol | Rhodopseudomonas palustris |
4.2.1.B28 | evolution | tetrahymanol synthase (Ths) is found in a variety of bacterial genomes, including aerobic methanotrophs, nitrite-oxidizers, and sulfate-reducers, and in a subset of aquatic and terrestrial metagenomes. The potential to produce tetrahymanol is more widespread in the bacterial domain than previously thought. Ths is not encoded in any eukaryotic genomes, nor is it homologous to eukaryotic squalene-tetrahymanol cyclase (EC 4.2.1.123), which catalyzes the cyclization of squalene directly to tetrahymanol | Methylotuvimicrobium alcaliphilum |
4.2.1.B28 | additional information | hopanoids, tetrahymanol, and sterol structures observed in Methylomicrobium alcaliphilum strain 20Z, overview | Methylotuvimicrobium alcaliphilum |
4.2.1.B28 | physiological function | tetrahymanol, whose catalysis is synthesized by tetrahymanol synthase, is a polycyclic triterpenoid lipid. Enzyme tetrahymanol synthase (Ths) functions on hopenes rather than squalene, it first demethylates squalene to form C23-norsqualene, which is then converted to a demethyl derivative of tetrahymanol by squalene-hopene cyclase (Shc, EC 5.4.99.17). This pathway requires the addition of a methyl group at C-23 to form tetrahymanol. Enzyme Shc cyclizes squalene to diploptene, and Ths then expands the E ring to form tetrahymanol, overview | Rhodopseudomonas palustris |
4.2.1.B28 | physiological function | tetrahymanol, whose catalysis is synthesized by tetrahymanol synthase, is a polycyclic triterpenoid lipid. Enzyme tetrahymanol synthase (Ths) functions on hopenes rather than squalene, it first demethylates squalene to form C23-norsqualene, which is then converted to a demethyl derivative of tetrahymanol by squalene-hopene cyclase (Shc, EC 5.4.99.17). This pathway requires the addition of a methyl group at C-23 to form tetrahymanol. Enzyme Shc cyclizes squalene to diploptene, and Ths then expands the E ring to form tetrahymanol, overview | Methylotuvimicrobium alcaliphilum |
4.2.1.123 | evolution | the eukaryotic squalene-tetrahymanol cyclase is homologous to the bacterial tetrahymanol synthase (Ths), that is not encoded in any eukaryotic genomes. Bacterial tetrahymanol synthase (Ths) is found in a variety of bacterial genomes, including aerobic methanotrophs, nitrite-oxidizers, and sulfate-reducers, and in a subset of aquatic and terrestrial metagenomes | Tetrahymena thermophila |
4.2.1.123 | physiological function | eukaryotic squalene-tetrahymanol cyclase catalyzes the cyclization of squalene directly to tetrahymanol, a polycyclic triterpenoid lipid, the enzyme catalyzes the cyclization of squalene directly to tetrahymanol | Tetrahymena thermophila |