EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.3.3 | expressed in Escherichia coli Rosetta 2 cells | Latilactobacillus sakei |
4.1.3.3 | expressed in Escherichia coli Rosetta 2 cells | Limosilactobacillus antri |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.3.3 | G210S/Y213G | the mutant shows reduced activity compared to the wild type enzyme | Limosilactobacillus antri |
4.1.3.3 | G211S | the mutant shows reduced activity compared to the wild type enzyme | Limosilactobacillus antri |
4.1.3.3 | P193Y | the mutant shows reduced activity compared to the wild type enzyme | Limosilactobacillus antri |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.3.3 | 0.27 | - |
N-acetylneuraminate | mutant enzyme P193Y, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 0.32 | - |
N-acetylneuraminate | wild type enzyme, at pH 7.0 and 37°C | Latilactobacillus sakei | |
4.1.3.3 | 0.5 | - |
pyruvate | mutant enzyme P193Y, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 1.1 | - |
pyruvate | wild type enzyme, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 1.1 | - |
N-acetylneuraminate | wild type enzyme, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 1.2 | - |
N-acetylneuraminate | mutant enzyme G211S, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 2 | - |
N-acetylneuraminate | mutant enzyme G210S/Y213G, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 4.3 | - |
pyruvate | mutant enzyme G210S/Y213G, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 12.5 | - |
pyruvate | wild type enzyme, at pH 7.0 and 37°C | Latilactobacillus sakei | |
4.1.3.3 | 272 | - |
N-acetyl-D-mannosamine | wild type enzyme, at pH 7.0 and 37°C | Latilactobacillus sakei | |
4.1.3.3 | 333 | - |
N-acetyl-D-mannosamine | wild type enzyme, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 915 | - |
N-acetyl-D-mannosamine | mutant enzyme P193Y, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 3200 | - |
N-acetyl-D-mannosamine | mutant enzyme G210S/Y213G, at pH 7.0 and 37°C | Limosilactobacillus antri |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.3.3 | 140000 | - |
gel filtration | Latilactobacillus sakei |
4.1.3.3 | 140000 | - |
gel filtration | Limosilactobacillus antri |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.3.3 | N-acetylneuraminate | Latilactobacillus sakei | 100% activity | N-acetyl-D-mannosamine + pyruvate | - |
r | |
4.1.3.3 | N-acetylneuraminate | Limosilactobacillus antri | 100% activity | N-acetyl-D-mannosamine + pyruvate | - |
r | |
4.1.3.3 | N-acetylneuraminate | Latilactobacillus sakei 23K | 100% activity | N-acetyl-D-mannosamine + pyruvate | - |
r | |
4.1.3.3 | N-acetylneuraminate | Limosilactobacillus antri DSMZ 16041 | 100% activity | N-acetyl-D-mannosamine + pyruvate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.3.3 | Latilactobacillus sakei | - |
- |
- |
4.1.3.3 | Latilactobacillus sakei 23K | - |
- |
- |
4.1.3.3 | Limosilactobacillus antri | C8P490 | - |
- |
4.1.3.3 | Limosilactobacillus antri DSMZ 16041 | C8P490 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.3.3 | Ni2+-chelating affinity column chromatography | Latilactobacillus sakei |
4.1.3.3 | Ni2+-chelating affinity column chromatography | Limosilactobacillus antri |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.3.3 | 2-deoxy-D-glucose + pyruvate | 1.2% activity compared to N-acetylneuraminate | Limosilactobacillus antri | ? | - |
r | |
4.1.3.3 | 2-deoxy-D-glucose + pyruvate | 33% activity compared to N-acetylneuraminate | Latilactobacillus sakei | ? | - |
r | |
4.1.3.3 | 2-deoxy-D-glucose + pyruvate | 33% activity compared to N-acetylneuraminate | Latilactobacillus sakei 23K | ? | - |
r | |
4.1.3.3 | 2-deoxy-D-glucose + pyruvate | 1.2% activity compared to N-acetylneuraminate | Limosilactobacillus antri DSMZ 16041 | ? | - |
r | |
4.1.3.3 | D-arabinose + pyruvate | 1.0% activity compared to N-acetylneuraminate | Latilactobacillus sakei | 3-deoxy-D-manno-2-octulosonic acid | - |
r | |
4.1.3.3 | D-arabinose + pyruvate | 1.5% activity compared to N-acetylneuraminate | Limosilactobacillus antri | 3-deoxy-D-manno-2-octulosonic acid | - |
r | |
4.1.3.3 | D-mannose + pyruvate | 10% activity compared to N-acetylneuraminate | Limosilactobacillus antri | ? | - |
r | |
4.1.3.3 | D-mannose + pyruvate | 95% activity compared to N-acetylneuraminate | Latilactobacillus sakei | ? | - |
r | |
4.1.3.3 | D-mannose + pyruvate | 95% activity compared to N-acetylneuraminate | Latilactobacillus sakei 23K | ? | - |
r | |
4.1.3.3 | D-mannose + pyruvate | 10% activity compared to N-acetylneuraminate | Limosilactobacillus antri DSMZ 16041 | ? | - |
r | |
4.1.3.3 | D-xylose + pyruvate | 0.6% activity compared to N-acetylneuraminate | Limosilactobacillus antri | ? | - |
r | |
4.1.3.3 | D-xylose + pyruvate | 2.4% activity compared to N-acetylneuraminate | Latilactobacillus sakei | ? | - |
r | |
4.1.3.3 | D-xylose + pyruvate | 2.4% activity compared to N-acetylneuraminate | Latilactobacillus sakei 23K | ? | - |
r | |
4.1.3.3 | D-xylose + pyruvate | 0.6% activity compared to N-acetylneuraminate | Limosilactobacillus antri DSMZ 16041 | ? | - |
r | |
4.1.3.3 | L-gulose + pyruvate | 0.7% activity compared to N-acetylneuraminate | Limosilactobacillus antri | ? | - |
r | |
4.1.3.3 | L-gulose + pyruvate | 3.6% activity compared to N-acetylneuraminate | Latilactobacillus sakei | ? | - |
r | |
4.1.3.3 | L-gulose + pyruvate | 3.6% activity compared to N-acetylneuraminate | Latilactobacillus sakei 23K | ? | - |
r | |
4.1.3.3 | L-gulose + pyruvate | 0.7% activity compared to N-acetylneuraminate | Limosilactobacillus antri DSMZ 16041 | ? | - |
r | |
4.1.3.3 | N-acetylneuraminate | 100% activity | Latilactobacillus sakei | N-acetyl-D-mannosamine + pyruvate | - |
r | |
4.1.3.3 | N-acetylneuraminate | 100% activity | Limosilactobacillus antri | N-acetyl-D-mannosamine + pyruvate | - |
r | |
4.1.3.3 | N-acetylneuraminate | 100% activity | Latilactobacillus sakei 23K | N-acetyl-D-mannosamine + pyruvate | - |
r | |
4.1.3.3 | N-acetylneuraminate | 100% activity | Limosilactobacillus antri DSMZ 16041 | N-acetyl-D-mannosamine + pyruvate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.3.3 | homotetramer | 4 * 35000, SDS-PAGE | Latilactobacillus sakei |
4.1.3.3 | homotetramer | 4 * 35000, SDS-PAGE | Limosilactobacillus antri |
4.1.3.3 | homotetramer | 4 * 33500, electrospray ionization mass spectrometry | Latilactobacillus sakei |
4.1.3.3 | homotetramer | 4 * 35700, electrospray ionization mass spectrometry | Limosilactobacillus antri |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.3.3 | NAL | - |
Latilactobacillus sakei |
4.1.3.3 | NAL | - |
Limosilactobacillus antri |
4.1.3.3 | Neu5Ac aldolase | - |
Latilactobacillus sakei |
4.1.3.3 | Neu5Ac aldolase | - |
Limosilactobacillus antri |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.3.3 | 40 | - |
in synthetic reaction | Latilactobacillus sakei |
4.1.3.3 | 50 | - |
in synthetic reaction | Latilactobacillus sakei |
4.1.3.3 | 60 | - |
in synthetic and hydrolytic reactions | Limosilactobacillus antri |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.3.3 | 42 | - |
melting temperature in 100 mM phosphate buffer pH 7.0 | Latilactobacillus sakei |
4.1.3.3 | 65 | - |
melting temperature in 100 mM phosphate buffer pH 7.0 | Limosilactobacillus antri |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.3.3 | 0.032 | - |
N-acetylneuraminate | mutant enzyme G211S, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 0.13 | - |
N-acetylneuraminate | mutant enzyme P193Y, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 0.47 | - |
N-acetylneuraminate | mutant enzyme G210S/Y213G, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 0.9 | - |
N-acetylneuraminate | wild type enzyme, at pH 7.0 and 37°C | Latilactobacillus sakei | |
4.1.3.3 | 0.99 | - |
pyruvate | mutant enzyme P193Y, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 1.9 | - |
N-acetylneuraminate | wild type enzyme, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 2.4 | - |
N-acetyl-D-mannosamine | mutant enzyme P193Y, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 3 | - |
pyruvate | mutant enzyme G210S/Y213G, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 3.9 | - |
pyruvate | wild type enzyme, at pH 7.0 and 37°C | Latilactobacillus sakei | |
4.1.3.3 | 4.6 | - |
N-acetyl-D-mannosamine | wild type enzyme, at pH 7.0 and 37°C | Latilactobacillus sakei | |
4.1.3.3 | 5.4 | - |
N-acetyl-D-mannosamine | wild type enzyme, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 5.6 | - |
pyruvate | wild type enzyme, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 15.3 | - |
N-acetyl-D-mannosamine | mutant enzyme G210S/Y213G, at pH 7.0 and 37°C | Limosilactobacillus antri |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.3.3 | 6 | 7 | - |
Latilactobacillus sakei |
4.1.3.3 | 6 | 7 | - |
Limosilactobacillus antri |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.3.3 | 0.002 | - |
N-acetyl-D-mannosamine | mutant enzyme P193Y, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 0.004 | - |
N-acetyl-D-mannosamine | mutant enzyme G210S/Y213G, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 0.016 | - |
N-acetyl-D-mannosamine | wild type enzyme, at pH 7.0 and 37°C | Latilactobacillus sakei | |
4.1.3.3 | 0.016 | - |
N-acetyl-D-mannosamine | wild type enzyme, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 0.026 | - |
N-acetylneuraminate | mutant enzyme G211S, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 0.23 | - |
N-acetylneuraminate | mutant enzyme G210S/Y213G, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 0.31 | - |
pyruvate | wild type enzyme, at pH 7.0 and 37°C | Latilactobacillus sakei | |
4.1.3.3 | 0.48 | - |
N-acetylneuraminate | mutant enzyme P193Y, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 0.71 | - |
pyruvate | mutant enzyme G210S/Y213G, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 1.7 | - |
N-acetylneuraminate | wild type enzyme, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 1.98 | - |
pyruvate | mutant enzyme P193Y, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.1.3.3 | 2.8 | - |
N-acetylneuraminate | wild type enzyme, at pH 7.0 and 37°C | Latilactobacillus sakei | |
4.1.3.3 | 5.1 | - |
pyruvate | wild type enzyme, at pH 7.0 and 37°C | Limosilactobacillus antri |