EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.99.8 | gene LdCAS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in a sterol-engineered Saccharomyces cerevisiae strains PA14, TM097, and TM122, that are derived from strain TM1 in the S288c / BY4742 background. Yeast strain PA14 is derived from strain TM1 by knocking out the TRP1 gene using CRISPR/Cas9 | Laurencia dendroidea |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.8 | (3S)-2,3-epoxy-2,3-dihydrosqualene | Laurencia dendroidea | - |
cycloartenol | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.8 | Laurencia dendroidea | A0A1I9Q605 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.8 | (3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Laurencia dendroidea | cycloartenol | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.8 | CAS | - |
Laurencia dendroidea |
5.4.99.8 | LdCAS | - |
Laurencia dendroidea |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.4.99.8 | evolution | the enzyme belongs to the family of 2,3-oxidosqualene cyclases (OSCs). A key step in the synthesis of cholesterol in red algae may be the reduction of the C-24(25) double bond by a DHCR24-like enzyme | Laurencia dendroidea |
5.4.99.8 | metabolism | profiling of Laurencia dendroidea reveals cholesterol as the major sterol accumulating in this species, implicating red seaweeds contain a hybrid sterol synthesis pathway in which the phytosterol precursor cycloartenol is converted into the major animal sterol cholesterol | Laurencia dendroidea |
5.4.99.8 | additional information | steroid profiling by GC-MS analysis | Laurencia dendroidea |