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Literature summary extracted from

  • Kumar, A.; Fogelman, E.; Weissberg, M.; Tanami, Z.; Veilleux, R.E.; Ginzberg, I.
    Lanosterol synthase-like is involved with differential accumulation of steroidal glycoalkaloids in potato (2017), Planta, 246, 1189-1202 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.7 gene LAS1, recombinant expression in Solanum tuberosum leaves using the transformation method with Agrobacterium tumefaciens strain EHA 105. Overexpression of the Arabidopsis LAS in transgenic potato modifies phytosterol and steroidal glycoalkaloid metabolite levels and the transcript levels of the respective biosynthetic genes. LAS1 pverexpression in Solanum tnerosum leaves results in increased steroidal glycoalkaloid level and reduced phytosterol level in the leaves, while the steroidal glycoalkaloid level in the tuber flesh is reduced Arabidopsis thaliana
5.4.99.7 identification of a putative LAS gene from potato (StLAS-like) and OSC activity verification in a yeast complementation assay by expression of the potato enzyme in LAS-deficient Saccharomyces cerevisiae strain GIL77. Analyses of StLAS-like transcript levels and StLAS-like::GUS reporter assays indicate different spatial expression in potato. Promoter sequence and activity analysis Solanum tuberosum

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.7 additional information overexpression of Arabidopsis thaliana AtLAS in leaves via transformation by Agrobacterium tumefaciens strain EHA 105, where the endogenic StLAS-like is not expressed, results in increased steroidal glycoalkaloid level and reduced phytosterol level in the leaves, while the steroidal glycoalkaloid level in the tuber flesh is reduced Solanum tuberosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.7 (3S)-2,3-epoxy-2,3-dihydrosqualene Arabidopsis thaliana
-
 lanosterol
-
 r
5.4.99.7 (3S)-2,3-epoxy-2,3-dihydrosqualene Solanum tuberosum
-
 lanosterol
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.7 Arabidopsis thaliana Q1G1A4
-
-
5.4.99.7 Solanum tuberosum A0A1B0YWV6 cv. Desiree
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.99.7 leaf
-
 Arabidopsis thaliana
-
5.4.99.7 tuber
-
 Solanum tuberosum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.7 (3S)-2,3-epoxy-2,3-dihydrosqualene
-
 Arabidopsis thaliana lanosterol
-
 r
5.4.99.7 (3S)-2,3-epoxy-2,3-dihydrosqualene
-
 Solanum tuberosum lanosterol
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.4.99.7 AtLAS
-
 Arabidopsis thaliana
5.4.99.7 LAS
-
 Arabidopsis thaliana
5.4.99.7 LAS
-
 Solanum tuberosum
5.4.99.7 LAS1
-
 Arabidopsis thaliana
5.4.99.7 tuber-specific StLAS-like
-
 Solanum tuberosum

General Information

EC Number General Information Comment Organism
5.4.99.7 evolution the enzyme belongs to the product-specific oxidosqualene cyclases (OSCs) that constitute a significant metabolic branch point which is the initial step of triterpene chemical diversity Arabidopsis thaliana
5.4.99.7 evolution the enzyme belongs to the product-specific oxidosqualene cyclases (OSCs) that constitute a significant metabolic branch point which is the initial step of triterpene chemical diversity Solanum tuberosum
5.4.99.7 metabolism phytosterol homeostasis may be maintained in leaves through diversion of intermediates into glycoalkaloid biosynthesis, whereas in tuber flesh, excess intermediates are catalyzed by tuber-specific StLAS-like, resulting in low tuber glycoalkaloids. The enzyme as product-specific oxidosqualene cyclase constitutes a significant metabolic branch point which is the initial step of triterpene chemical diversity Solanum tuberosum
5.4.99.7 metabolism the enzyme as product-specific oxidosqualene cyclase constitutes a significant metabolic branch point which is the initial step of triterpene chemical diversity Arabidopsis thaliana