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Literature summary extracted from

  • Bruno, M.; Al-Babili, S.
    On the substrate specificity of the rice strigolactone biosynthesis enzyme DWARF27 (2016), Planta, 243, 1429-1440 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.2.1.14 FeSO4 1 mM, present in assay Oryza sativa Japonica Group

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.2.1.14 expression in Escherichia coli Oryza sativa Japonica Group

Organism

EC Number Organism UniProt Comment Textmining
5.2.1.14 Oryza sativa Japonica Group C7AU21
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.2.1.14 9-cis-beta-carotene reaction is reversible, the conversion of 9-cis- into all-trans-beta-carotene is the preferred reaction Oryza sativa Japonica Group all-trans-beta-carotene
-
 r
5.2.1.14 all-trans-beta,beta-cryptoxanthin
-
 Oryza sativa Japonica Group 9-cis-beta,beta-cryptoxanthin
-
 r
5.2.1.14 all-trans-beta-carotene
-
 Oryza sativa Japonica Group 9-cis-beta-carotene
-
 r
5.2.1.14 alpha-carotene
-
 Oryza sativa Japonica Group 9-cis-alpha-carotene + 9'-cis-alpha-carotene
-
 ?
5.2.1.14 additional information the enzyme is strictly double bond-specific, solely targeting the C9-C10 double bond. DWARF27 does not introduce a 9-cis-double bond in 13-cis- or 15-cis-beta-carotene. Substrates are bicyclic carotenoids, including beta-, alpha-carotene and beta,beta-cryptoxanthin, that contain at least one unsubstituted beta-ionone ring Oryza sativa Japonica Group ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2.1.14 6.4
-
 MOPS buffer Oryza sativa Japonica Group