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Literature summary extracted from

  • Mafu, S.; Ding, Y.; Murphy, K.M.; Yaacoobi, O.; Addison, J.B.; Wang, Q.; Shen, Z.; Briggs, S.P.; Bohlmann, J.; Castro-Falcon, G.; Hughes, C.C.; Betsiashvili, M.; Huffaker, A.; Schmelz, E.A.; Zerbe, P.
    Discovery, biosynthesis and stress-related accumulation of dolabradiene-derived defenses in maize (2018), Plant Physiol., 176, 2677-2690 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.159 expression in Escherichia coli Zea mays
4.2.3.196 expression in Escherichia coli BL21DE3-C41 cells Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.159 15,16-epoxydolabrene + O2 + [reduced NADPH-hemoprotein reductase] Zea mays the enzyme is involved in biosynthesis of dolabralexin. It catalyses the epoxidation of dolabradiene at C-16, followed by hydroxylation at C-3 3beta-hydroxy-15,16-epoxydolabrene + H2O + [oxidized NADPH-hemoprotein reductase]
-
 ?
1.14.14.159 dolabradiene + O2 + [reduced NADPH-hemoprotein reductase] Zea mays the enzyme is involved in biosynthesis of dolabralexin. It catalyses the epoxidation of dolabradiene at C-16, followed by hydroxylation at C-3 15,16-epoxydolabrene + H2O + [oxidized NADPH-hemoprotein reductase]
-
 ?
4.2.3.196 ent-copalyl diphosphate Zea mays the enzyme is involved in the biosynthesis of dolabralexins (type of antifungal phytoalexins) dolabradiene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.159 Zea mays
-
-
-
4.2.3.196 Zea mays A0A3L6GCD0
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.159 root
-
 Zea mays
-
4.2.3.196 root
-
 Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.159 15,16-epoxydolabrene + O2 + [reduced NADPH-hemoprotein reductase] the enzyme is involved in biosynthesis of dolabralexin. It catalyses the epoxidation of dolabradiene at C-16, followed by hydroxylation at C-3 Zea mays 3beta-hydroxy-15,16-epoxydolabrene + H2O + [oxidized NADPH-hemoprotein reductase]
-
 ?
1.14.14.159 15,16-epoxydolabrene + O2 + [reduced NADPH-hemoprotein reductase] the enzyme catalyses the epoxidation of dolabradiene at C-16, followed by hydroxylation at C-3 Zea mays 3beta-hydroxy-15,16-epoxydolabrene + H2O + [oxidized NADPH-hemoprotein reductase]
-
 ?
1.14.14.159 dolabradiene + O2 + [reduced NADPH-hemoprotein reductase] the enzyme is involved in biosynthesis of dolabralexin. It catalyses the epoxidation of dolabradiene at C-16, followed by hydroxylation at C-3 Zea mays 15,16-epoxydolabrene + H2O + [oxidized NADPH-hemoprotein reductase]
-
 ?
1.14.14.159 dolabradiene + O2 + [reduced NADPH-hemoprotein reductase] the enzyme catalyses the epoxidation of dolabradiene at C-16, followed by hydroxylation at C-3 Zea mays 15,16-epoxydolabrene + H2O + [oxidized NADPH-hemoprotein reductase]
-
 ?
4.2.3.196 ent-copalyl diphosphate
-
 Zea mays dolabradiene + diphosphate
-
 ?
4.2.3.196 ent-copalyl diphosphate the enzyme is involved in the biosynthesis of dolabralexins (type of antifungal phytoalexins) Zea mays dolabradiene + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.159 CYP71Z16
-
 Zea mays
4.2.3.196 KSL4
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.159 cytochrome P-450 a cytochrome P-450 (heme thiolate) enzyme Zea mays

Expression

EC Number Organism Comment Expression
1.14.14.159 Zea mays no increase of transcript level after inoculation with live spores of Fusarium verticillioides and Fusarium graminearum additional information
1.14.14.159 Zea mays increased transcript levels under abiotic stress condition (root treatments with 1 mM CuSO4) up
4.2.3.196 Zea mays expression is induced by oxidative stress up
4.2.3.196 Zea mays transcript accumulation is up-regulated in response to elicitation with the fungal pathogens Fusarium verticillioides and Fusarium graminearum up

General Information

EC Number General Information Comment Organism
1.14.14.159 metabolism the enzyme is involved in biosynthesis of dolabralexin Zea mays
4.2.3.196 metabolism the enzyme is involved in the biosynthesis of dolabralexins (type of antifungal phytoalexins) Zea mays