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Literature summary extracted from

  • Para, A.; Muhammad, D.; Orozco-Nunnelly, D.A.; Memishi, R.; Alvarez, S.; Naldrett, M.J.; Warpeha, K.M.
    The dehydratase ADT3 affects ROS homeostasis and cotyledon development (2016), Plant Physiol., 172, 1045-1060 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.91 additional information construction of an adt3 knockout mutant, phenotype, overview Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.91 cytosol mainly Arabidopsis thaliana 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.91 L-arogenate Arabidopsis thaliana
-
 L-phenylalanine + H2O + CO2
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 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.91 Arabidopsis thaliana Q9ZUY3
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.91 cotyledon
-
 Arabidopsis thaliana
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4.2.1.91 epidermis
-
 Arabidopsis thaliana
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4.2.1.91 leaf
-
 Arabidopsis thaliana
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4.2.1.91 seedling
-
 Arabidopsis thaliana
-
4.2.1.91 shoot apical meristem Arabidopsis thaliana
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.91 L-arogenate
-
 Arabidopsis thaliana L-phenylalanine + H2O + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.91 ADT3
-
 Arabidopsis thaliana
4.2.1.91 arogenate dehydratase3
-
 Arabidopsis thaliana
4.2.1.91 dehydratase ADT3
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
4.2.1.91 malfunction genome-wide proteomics of the adt3 mutant revealed a general downregulation of plastidic proteins and ROS-scavenging enzymes, corroborating the hypothesis that the ADT3 supply of Phe is required to control ROS concentration and distribution to protect cellular components. In addition, loss of ADT3 disrupts cotyledon epidermal patterning by affecting the number and expansion of pavement cells and stomata cell fate specification, severe alterations in mesophyll cells, which lack oil bodies and normal plastids, are observed. Loss of ADT3 disturbs epidermis development in the cotyledons of dark-grown seedlings. Mutant adt3 cotyledons exhibit abnormal subcellular features. Upregulation of the pathway leading to cuticle production is accompanied by an abnormal cuticle structure and/or deposition in the adt3 mutant. Such impairment results in an increase in cell permeability and provides a link to understand the cell defects in the adt3 cotyledon epidermis. Involvement of the cell wall in adt3 cell morphology, phenotype. The proteomic analysis reveals that adt3-1 mutation causes alteration of the expression of 1333 proteins, 608 of which are downregulated and 725 of which are upregulated, overview Arabidopsis thaliana
4.2.1.91 physiological function arogenate dehydratase3, ADT3, plays a critical role for the phenylalanine (Phe) biosynthetic activity coordinating reactive oxygen species (ROS) homeostasis and cotyledon development in etiolated Arabidopsis thaliana seedlings. Isozyme ADT3 is expressed in the cotyledon and shoot apical meristem, mainly in the cytosol, the epidermis of adt3 cotyledons contains higher levels of ROS. L-Phe might play an additional role in supplying nutrients to the young seedling. ADT3 accumulates in the cytosol and is required to maintain ROS homeostasis Arabidopsis thaliana