Any feedback?
Literature summary extracted from
- Isolation and functional characterization of a methyl jasmonate-responsive 3-carene synthase from Lavandula x intermedia (2017), Plant Mol. Biol., 93, 641-657 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.3.107 | gene Li3CARS, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli | Lavandula x intermedia |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.3.107 | additional information | - |
additional information | Michaelis-Menten kinetics | Lavandula x intermedia | |
| 4.2.3.107 | 0.00369 | - |
geranyl diphosphate | recombinant His-tagged enzyme, pH 7.2, temperature not specified in the publication | Lavandula x intermedia |  |
| 4.2.3.107 | 0.0143 | - |
neryl diphosphate | recombinant His-tagged enzyme, pH 7.2, temperature not specified in the publication | Lavandula x intermedia | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.3.107 | Mg2+ | required | Lavandula x intermedia | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.107 | geranyl diphosphate | Lavandula x intermedia | - |
(+)-car-3-ene + diphosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.3.107 | Lavandula x intermedia | A0A1V0D8Y5 | cv. Grosso | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.3.107 | recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Lavandula x intermedia |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.3.107 | flower | - |
Lavandula x intermedia | - |
| 4.2.3.107 | leaf | - |
Lavandula x intermedia | - |
| 4.2.3.107 | additional information | Li3CARS transcripts are highly abundant in leaves (16fold) as compared to flower tissues | Lavandula x intermedia | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.107 | geranyl diphosphate | - |
Lavandula x intermedia | (+)-car-3-ene + diphosphate | - |
? | |
| 4.2.3.107 | geranyl diphosphate | geranyl diphosphate is converted mainly to 3-carene | Lavandula x intermedia | (+)-car-3-ene + diphosphate | - |
? | |
| 4.2.3.107 | additional information | the recombinant Li3CARS converted geranyl diphosphate (GPP) into 3-carene as the major product, Li3CARS also accepts neryl diphosphate (NPP) as a substrate to produce multiple products including a small amount of 3-carene. The catalytic efficiency of Li3CARS to produce 3-carene is about 13fold higher for GPP than NPP. Ligand identification by GC-MS analysis | Lavandula x intermedia | ? | - |
? | |
| 4.2.3.107 | neryl diphosphate | low activity, neryl diphosphate is transformed into limonene (33%), alpha-terpinolene (29%) and alpha-thujene (20%) as main products, and minor amounts of a few other monoterpenes including 3-carene (8.5%) | Lavandula x intermedia | limonene + diphosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.3.107 | ? | x * 64900, about, sequence calculation from enzyme sequence excluding transit peptides, x * 69600, about, full-length enzyme, sequence calculation | Lavandula x intermedia |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.3.107 | 3-carene synthase | - |
Lavandula x intermedia |
| 4.2.3.107 | 3CARS | - |
Lavandula x intermedia |
| 4.2.3.107 | Li3CARS | - |
Lavandula x intermedia |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.3.107 | 0.636 | - |
neryl diphosphate | recombinant His-tagged enzyme, pH 7.2, temperature not specified in the publication | Lavandula x intermedia | |
| 4.2.3.107 | 2.07 | - |
geranyl diphosphate | recombinant His-tagged enzyme, pH 7.2, temperature not specified in the publication | Lavandula x intermedia | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.107 | 7.2 | - |
recombinant enzyme | Lavandula x intermedia |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.107 | 5.5 | 8 | activity range, and below, profile overview | Lavandula x intermedia |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 4.2.3.107 | Lavandula x intermedia | sequence calculation | - |
5.08 |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 4.2.3.107 | Lavandula x intermedia | enzyme 3-carene synthase from Lavandula intermedia hybrid is methyl jasmonate (MeJA) responsive, the 3CARS expression is upregulated 1.18 to 3.8fold with MeJA 8-72 h post-treatment | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.2.3.107 | evolution | evolutionary inference from Lavandula TPS genes, Li3CARS is highly homologous to the Lavandula angustifolia limonene synthase (LaLIMS), and is clustered with this gene under angiosperm mTPSs (TPS-b) subfamily | Lavandula x intermedia |
| 4.2.3.107 | metabolism | biosynthesis of monoterpenes catalyzed by recombinant Li3CARS from geranyl diphosphate (GPP, derived from plastidial 2-C-methyl-D-erythritol-4-phosphate pathway) and neryl diphosphate (NPP) | Lavandula x intermedia |
| 4.2.3.107 | additional information | homology-based molecular modeling of Li3CARS using the crystal structure of Salvia officinalis bornyl pyrophosphate synthase (SoBPPS) (PDB ID 1N1Z), containing the substrate analogue diphosphate (POP), as template | Lavandula x intermedia |
| 4.2.3.107 | physiological function | lavenders produce essential oils consisting mainly of monoterpenes, including the potent antimicrobial and insecticidal monoterpene 3-carene. Gene Li3CARS encodes a leaf-specific, methyl jasmonate (MeJA)-responsive monoterpene synthase from Lavandula x intermedia. The transcriptional activity of Li3CARS correlates with 3-carene production. enzyme Li3CARS may play a defensive role in lavenders | Lavandula x intermedia |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.3.107 | 44.5 | - |
neryl diphosphate | recombinant His-tagged enzyme, pH 7.2, temperature not specified in the publication | Lavandula x intermedia | |
| 4.2.3.107 | 561 | - |
geranyl diphosphate | recombinant His-tagged enzyme, pH 7.2, temperature not specified in the publication | Lavandula x intermedia | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
