Literature summary extracted from

Feiz, L.; Williams-Carrier, R.; Belcher, S.; Montano, M.; Barkan, A.; Stern, D.B.
A protein with an inactive pterin-4a-carbinolamine dehydratase domain is required for Rubisco biogenesis in plants (2014), Plant J., 80, 862-869 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.2.1.96	gene 100277322, the raf2 locus corresponds to GRMZM2G139123, an intron-less locus on chromosome 1	Zea mays

Protein Variants

EC Number	Protein Variants	Comment	Organism
4.2.1.96	additional information	raf2 mutant phenotype, overview	Zea mays

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.2.1.96	Zea mays	B6TSX9	-	-

Synonyms

EC Number	Synonyms	Comment	Organism
4.2.1.96	4-alpha-hydroxytetrahydrobiopterin dehydratase	UniProt	Zea mays
4.2.1.96	PCD	-	Zea mays
4.2.1.96	Pterin-4a-carbinolamine dehydratase	-	Zea mays

General Information

EC Number	General Information	Comment	Organism
4.2.1.96	malfunction	a Mu transposon insertion in the Zea mays (maize) gene encoding a chloroplast dimerization co-factor of hepatocyte nuclear factor 1 (DCoH)/pterin-4a-carbinolamine dehydratases (PCD)-like protein is the causative mutation in a seedling-lethal, Rubisco-deficient mutant named Rubisco accumulation factor 2 (raf2-1). In raf2 mutants newly synthesized Rubisco large subunit accumulates in a high-molecular weight complex, the formation of which requires a specific chaperonin 60-kDa isoform. raf2 Mutant phenotype, overview	Zea mays
4.2.1.96	physiological function	the maize gene Rubisco accumulation factor 2 (raf2) is required for Rubisco biogenesis. RAF2 is conserved among photosynthetic eukaryotes and is related to a mitochondrial protein: dimerization co-factor of hepatocyte nuclear factor 1 (DCoH)/pterin-4a-carbinolamine dehydratases (PCD)-like protein. The pterin-4a-carbinolamine dehydratases (PCD)-like protein RAF2 lacks PCD activity acquires a chloroplast-specific domain. Effect of RAF2 on the accumulation of rbcL and rbcs transcripts is investigated by gel blot analysis of total seedling leaf RNA from wild-type and raf2 mutants	Zea mays

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Release 2023.1 (January 2023)