Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mao, H.; Liu, J.; Ren, F.; Peters, R.J.; Wang, Q.
    Characterization of CYP71Z18 indicates a role in maize zealexin biosynthesis (2016), Phytochemistry, 121, 4-10 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.160 CYP71Z18 is expressed in the C41 (DE3) OverExpress strain of Escherichia coli (Lucigen) Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.160 (S)-beta-macrocarpene + 3 O2 + 3 [reduced NADPH-hemoprotein reductase] Zea mays the enzyme sequentially oxidizes (S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, a maize phytoalexin that provides biochemical protection against fungal infection, overall reaction zealexin A1 + 4 H2O + 3 [oxidized NADPH-hemoprotein reductase]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.160 Zea mays B4FVP3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.160 (4S)-4-(5,5-dimethylcyclohex-1-en-1-yl)cyclohex-1-ene-1-carbaldehyde + O2 + [reduced NADPH-hemoprotein reductase] the enzyme sequentially oxidizes(S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1 Zea mays zealexin A1 + H2O + [oxidized NADPH-hemoprotein reductase]
-
 ?
1.14.14.160 (S)-beta-macrocarpene + 3 O2 + 3 [reduced NADPH-hemoprotein reductase] the enzyme sequentially oxidizes (S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, a maize phytoalexin that provides biochemical protection against fungal infection, overall reaction Zea mays zealexin A1 + 4 H2O + 3 [oxidized NADPH-hemoprotein reductase]
-
 ?
1.14.14.160 (S)-beta-macrocarpene + 3 O2 + 3 [reduced NADPH-hemoprotein reductase] the enzyme sequentially oxidizes(S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, overall reaction Zea mays zealexin A1 + 4 H2O + 3 [oxidized NADPH-hemoprotein reductase]
-
 ?
1.14.14.160 (S)-beta-macrocarpene + O2 + [reduced NADPH-hemoprotein reductase] the enzyme sequentially oxidizes(S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1 Zea mays [(4S)-4-(5,5-dimethylcyclohex-1-en-1-yl)-cyclohex-1-en-1-yl]methanol + H2O + [oxidized NADPH-hemoprotein reductase]
-
 ?
1.14.14.160 [(4S)-4-(5,5-dimethylcyclohex-1-en-1-yl)-cyclohex-1-en-1-yl] methanol + O2 + [reduced NADPH-hemoprotein reductase] the enzyme sequentially oxidizes(S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1 Zea mays (4S)-4-(5,5-dimethylcyclohex-1-en-1-yl)cyclohex-1-ene-1-carbaldehyde + 2 H2O + [oxidized NADPH-hemoprotein reductase]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.160 CYP71Z18
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.160 cytochrome P-450 a cytochrome P-450 (heme thiolate) enzyme Zea mays

Expression

EC Number Organism Comment Expression
1.14.14.160 Zea mays transcription is strongly induced by fungal infection up

General Information

EC Number General Information Comment Organism
1.14.14.160 metabolism the enzyme sequentially oxidizes (S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, a maize phytoalexin that provides biochemical protection against fungal infection Zea mays
1.14.14.160 physiological function the enzyme sequentially oxidizes (S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, a maize phytoalexin that provides biochemical protection against fungal infection Zea mays