BRENDA - Enzyme Database show

Multifunctional oxidosqualene cyclases and cytochrome P450 involved in the biosynthesis of apple fruit triterpenic acids

Andre, C.; Legay, S.; Deleruelle, A.; Nieuwenhuizen, N.; Punter, M.; Brendolise, C.; Cooney, J.; Lateur, M.; Hausman, J.; Larondelle, Y.; Laing, W.; New Phytol. 211, 1279-1294 (2016)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.14.126
gene CYP716A175, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, transient recombinant expression in Nicotiana benthamiana using the transformation method via Agrobacterium tumefaciens strain GV3101. The higher oxidizing efficiency of CYP716A175 for lupeol and beta-amyrin compared with germanicol preferentially drives the whole reaction chain to the production of betulinate and oleanolate rather than morolic acid. Transient expression in Saccharomyces cerevisiae shows the presence of the intermediate erythrodiol in addition to oleanolate, but not of oleanolic aldehyde. Sequential three-step oxidation reaction leading to oleanolate, low efficiency of CYP716A75 to oxidize erythrodiol in yeast
Malus domestica
5.4.99.B2
gene OSC1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis of the enzyme in apple fruits, functional recombinant expression in Nicotiana benthamiana via transformation by Agrobacterium tumefaciens strain GV3101 resulting in production of alpha-amyrin, beta-amyrin, and lupeol. MdOSC1 produces predominantly alpha-amyrin, beta-amyrin to a lesser extent, and small amounts of lupeol; gene OSC3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis of the enzyme in apple fruits, recombinant expression in Nicotiana benthamiana via transformation by Agrobacterium tumefaciens strain GV3101 resulting in production of alpha-amyrin, beta-amyrin, and lupeol
Malus domestica
5.4.99.34
gene OSC4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis of the enzyme in apple fruits, functional recombinant expression in Nicotiana benthamiana via transformation by Agrobacterium tumefaciens strain GV3101 resulting in production of germanicol, beta-amyrin, and lupeol
Malus domestica
5.4.99.39
gene OSC1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis of the enzyme in apple fruits, functional recombinant expression in Nicotiana benthamiana via transformation by Agrobacterium tumefaciens strain GV3101 resulting in production of alpha-amyrin, beta-amyrin, and lupeol. MdOSC1 produces predominantly alpha-amyrin, beta-amyrin to a lesser extent, and small amounts of lupeol
Malus domestica
5.4.99.41
gene OSC1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis of the enzyme in apple fruits, functional recombinant expression in Nicotiana benthamiana leaves via transformation by Agrobacterium tumefaciens strain GV3101 resulting in production of alpha-amyrin, beta-amyrin, and lupeol. Cells transiently expressing MdOSC5 cDNA are found to generate lupeol as a major component and beta-amyrin as a minor product, in a ratio of 95:5
Malus domestica
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.4.99.B2
additional information
transient expression of MdOSC1 and MdOSC5 together with CYP716A175 confirms the ratio between the different triterpene backbones observed after transient expression of MdOSC1 and MDOSC5 alone, overview
Malus domestica
5.4.99.39
additional information
transient expression of MdOSC1 and MdOSC5 together with CYP716A175 confirms the ratio between the different triterpene backbones observed after transient expression of MdOSC1 and MDOSC5 alone, overview
Malus domestica
5.4.99.41
additional information
transient expression of MdOSC1 and MdOSC5 together with CYP716A175 confirms the ratio between the different triterpene backbones observed after transient expression of MdOSC1 and MDOSC5 alone, overview
Malus domestica
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.126
alpha-amyrin + 3 O2 + 3 NADPH + 3 H+
Malus domestica
overall reaction
ursolate + 3 NADP+ + 4 H2O
-
-
?
1.14.14.126
beta-amyrin + 3 O2 + 3 NADPH + 3 H+
Malus domestica
overall reaction
oleanolate + 3 NADP+ + 4 H2O
-
-
?
1.14.14.126
germanicol + 3 O2 + 3 NADPH + 3 H+
Malus domestica
overall reaction
morolate + 3 NADP+ + 4 H2O
-
-
?
1.14.14.126
lupeol + 3 O2 + 3 NADPH + 3 H+
Malus domestica
overall reaction
betulinate + 3 NADP+ + 4 H2O
-
-
?
1.14.14.126
additional information
Malus domestica
CYP716A175 catalyses the C-28 oxidation of alpha-amyrin, beta-amyrin, lupeol and germanicol, producing ursolic acid, oleanolic acid, betulinic acid, and putatively morolic acid. CYP716A175 catalyses the three-step oxidation of triterpene backbones at the C-28 position
?
-
-
-
5.4.99.B2
(S)-2,3-oxidosqualene
Malus domestica
via dammarenyl cation
beta-amyrin
-
-
r
5.4.99.B2
(S)-2,3-oxidosqualene
Malus domestica
via dammarenyl cation
lupeol
-
-
r
5.4.99.B2
(S)-2,3-oxidosqualene
Malus domestica
via dammarenyl cation
alpha-amyrin
-
-
r
5.4.99.34
(3S)-2,3-epoxy-2,3-dihydrosqualene
Malus domestica
-
germanicol
-
-
r
5.4.99.34
additional information
Malus domestica
although the MdOSC4 gene was expressed in the skins of all apple varieties under investigation, no germanicol or derivatives were detected at the metabolite level
?
-
-
-
5.4.99.39
(S)-2,3-oxidosqualene
Malus domestica
via dammarenyl cation
beta-amyrin
-
-
r
5.4.99.41
(S)-2,3-oxidosqualene
Malus domestica
via dammarenyl cation, low activity
beta-amyrin
-
-
r
5.4.99.41
(S)-2,3-oxidosqualene
Malus domestica
via dammarenyl cation
lupeol
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.126
Malus domestica
EB148173
-
-
5.4.99.B2
Malus domestica
C7SCX0
hybrid
-
5.4.99.B2
Malus domestica
C7SCX2
hybrid, cultivars Merton Russet and Royal Gala, grown in New Zealand
-
5.4.99.34
Malus domestica
A0A142LT03
-
-
5.4.99.39
Malus domestica
C7SCX0
hybrid
-
5.4.99.41
Malus domestica
A0A142LT04
hybrid
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.126
additional information
gene expression pattern analysis of CYP716A175 in 20 apple cultivars
Malus domestica
-
5.4.99.B2
epidermis
;
Malus domestica
-
5.4.99.B2
fruit
;
Malus domestica
-
5.4.99.34
fruit
-
Malus domestica
-
5.4.99.34
additional information
although the MdOSC4 gene was expressed in the skins of all apple varieties under investigation, no germanicol or derivatives were detected at the metabolite level
Malus domestica
-
5.4.99.34
plant epidermis
-
Malus domestica
-
5.4.99.39
epidermis
-
Malus domestica
-
5.4.99.39
fruit
-
Malus domestica
-
5.4.99.41
epidermis
-
Malus domestica
-
5.4.99.41
fruit
-
Malus domestica
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.126
alpha-amyrin + 3 O2 + 3 NADPH + 3 H+
overall reaction
748772
Malus domestica
ursolate + 3 NADP+ + 4 H2O
-
-
-
?
1.14.14.126
beta-amyrin + 3 O2 + 3 NADPH + 3 H+
overall reaction
748772
Malus domestica
oleanolate + 3 NADP+ + 4 H2O
-
-
-
?
1.14.14.126
germanicol + 3 O2 + 3 NADPH + 3 H+
overall reaction
748772
Malus domestica
morolate + 3 NADP+ + 4 H2O
-
-
-
?
1.14.14.126
lupeol + 3 O2 + 3 NADPH + 3 H+
overall reaction
748772
Malus domestica
betulinate + 3 NADP+ + 4 H2O
-
-
-
?
1.14.14.126
additional information
CYP716A175 catalyses the C-28 oxidation of alpha-amyrin, beta-amyrin, lupeol and germanicol, producing ursolic acid, oleanolic acid, betulinic acid, and putatively morolic acid. CYP716A175 catalyses the three-step oxidation of triterpene backbones at the C-28 position
748772
Malus domestica
?
-
-
-
-
1.14.14.126
additional information
CYP716A175 catalyses the three-step oxidation of triterpene backbones at the C-28 position. CYP716A175 has a similar oxidizing capacity for the triterpene scaffold alpha-amyrin, beta-amyrin, and lupeol but not for germanicol
748772
Malus domestica
?
-
-
-
-
5.4.99.B2
(S)-2,3-oxidosqualene
via dammarenyl cation
748772
Malus domestica
beta-amyrin
-
-
-
r
5.4.99.B2
(S)-2,3-oxidosqualene
via dammarenyl cation
748772
Malus domestica
lupeol
-
-
-
r
5.4.99.B2
(S)-2,3-oxidosqualene
via dammarenyl cation
748772
Malus domestica
alpha-amyrin
-
-
-
r
5.4.99.B2
additional information
MdOSC1 is a mixed amyrin synthase, with a 5:1 ratio of alpha-amyrin to beta-amyrin. The alpha-amyrin to beta-amyrin to lupeol production ratio for MdOSC1 is 85:13:2
748772
Malus domestica
?
-
-
-
-
5.4.99.34
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
748772
Malus domestica
germanicol
-
-
-
r
5.4.99.34
(S)-2,3-oxidosqualene
via dammarenyl cation, low activity
748772
Malus domestica
beta-amyrin
-
-
-
r
5.4.99.34
(S)-2,3-oxidosqualene
via dammarenyl cation, low activity
748772
Malus domestica
lupeol
-
-
-
r
5.4.99.34
additional information
although the MdOSC4 gene was expressed in the skins of all apple varieties under investigation, no germanicol or derivatives were detected at the metabolite level
748772
Malus domestica
?
-
-
-
-
5.4.99.34
additional information
gene MdOSC4 encodes a multifunctional oxidosqualene cyclase producing an oleanane-type triterpene, putatively identified as germanicol, as well as beta-amyrin and lupeol, in the proportion 82:14:4
748772
Malus domestica
?
-
-
-
-
5.4.99.39
(S)-2,3-oxidosqualene
via dammarenyl cation
748772
Malus domestica
beta-amyrin
-
-
-
r
5.4.99.39
additional information
MdOSC1 is a mixed amyrin synthase, with a 5:1 ratio of alpha-amyrin to beta-amyrin. The alpha-amyrin to beta-amyrin to lupeol production ratio for MdOSC1 from (S)-2,3-oxidosqualene is 85:13:2
748772
Malus domestica
?
-
-
-
-
5.4.99.41
(S)-2,3-oxidosqualene
via dammarenyl cation, low activity
748772
Malus domestica
beta-amyrin
-
-
-
r
5.4.99.41
(S)-2,3-oxidosqualene
via dammarenyl cation
748772
Malus domestica
lupeol
-
-
-
r
5.4.99.41
additional information
MdOSC5 cyclizes 2,3-oxidosqualene into lupeol and beta-amyrin at a ratio of 95:5
748772
Malus domestica
?
-
-
-
-
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.126
cytochrome P450
-
Malus domestica
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.126
gene CYP716A175, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, transient recombinant expression in Nicotiana benthamiana using the transformation method via Agrobacterium tumefaciens strain GV3101. The higher oxidizing efficiency of CYP716A175 for lupeol and beta-amyrin compared with germanicol preferentially drives the whole reaction chain to the production of betulinate and oleanolate rather than morolic acid. Transient expression in Saccharomyces cerevisiae shows the presence of the intermediate erythrodiol in addition to oleanolate, but not of oleanolic aldehyde. Sequential three-step oxidation reaction leading to oleanolate, low efficiency of CYP716A75 to oxidize erythrodiol in yeast
Malus domestica
5.4.99.34
gene OSC4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis of the enzyme in apple fruits, functional recombinant expression in Nicotiana benthamiana via transformation by Agrobacterium tumefaciens strain GV3101 resulting in production of germanicol, beta-amyrin, and lupeol
Malus domestica
5.4.99.39
gene OSC1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis of the enzyme in apple fruits, functional recombinant expression in Nicotiana benthamiana via transformation by Agrobacterium tumefaciens strain GV3101 resulting in production of alpha-amyrin, beta-amyrin, and lupeol. MdOSC1 produces predominantly alpha-amyrin, beta-amyrin to a lesser extent, and small amounts of lupeol
Malus domestica
5.4.99.41
gene OSC1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis of the enzyme in apple fruits, functional recombinant expression in Nicotiana benthamiana leaves via transformation by Agrobacterium tumefaciens strain GV3101 resulting in production of alpha-amyrin, beta-amyrin, and lupeol. Cells transiently expressing MdOSC5 cDNA are found to generate lupeol as a major component and beta-amyrin as a minor product, in a ratio of 95:5
Malus domestica
5.4.99.B2
gene OSC1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis of the enzyme in apple fruits, functional recombinant expression in Nicotiana benthamiana via transformation by Agrobacterium tumefaciens strain GV3101 resulting in production of alpha-amyrin, beta-amyrin, and lupeol. MdOSC1 produces predominantly alpha-amyrin, beta-amyrin to a lesser extent, and small amounts of lupeol
Malus domestica
5.4.99.B2
gene OSC3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis of the enzyme in apple fruits, recombinant expression in Nicotiana benthamiana via transformation by Agrobacterium tumefaciens strain GV3101 resulting in production of alpha-amyrin, beta-amyrin, and lupeol
Malus domestica
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.126
cytochrome P450
-
Malus domestica
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.4.99.39
additional information
transient expression of MdOSC1 and MdOSC5 together with CYP716A175 confirms the ratio between the different triterpene backbones observed after transient expression of MdOSC1 and MDOSC5 alone, overview
Malus domestica
5.4.99.41
additional information
transient expression of MdOSC1 and MdOSC5 together with CYP716A175 confirms the ratio between the different triterpene backbones observed after transient expression of MdOSC1 and MDOSC5 alone, overview
Malus domestica
5.4.99.B2
additional information
transient expression of MdOSC1 and MdOSC5 together with CYP716A175 confirms the ratio between the different triterpene backbones observed after transient expression of MdOSC1 and MDOSC5 alone, overview
Malus domestica
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.126
alpha-amyrin + 3 O2 + 3 NADPH + 3 H+
Malus domestica
overall reaction
ursolate + 3 NADP+ + 4 H2O
-
-
?
1.14.14.126
beta-amyrin + 3 O2 + 3 NADPH + 3 H+
Malus domestica
overall reaction
oleanolate + 3 NADP+ + 4 H2O
-
-
?
1.14.14.126
germanicol + 3 O2 + 3 NADPH + 3 H+
Malus domestica
overall reaction
morolate + 3 NADP+ + 4 H2O
-
-
?
1.14.14.126
lupeol + 3 O2 + 3 NADPH + 3 H+
Malus domestica
overall reaction
betulinate + 3 NADP+ + 4 H2O
-
-
?
1.14.14.126
additional information
Malus domestica
CYP716A175 catalyses the C-28 oxidation of alpha-amyrin, beta-amyrin, lupeol and germanicol, producing ursolic acid, oleanolic acid, betulinic acid, and putatively morolic acid. CYP716A175 catalyses the three-step oxidation of triterpene backbones at the C-28 position
?
-
-
-
5.4.99.B2
(S)-2,3-oxidosqualene
Malus domestica
via dammarenyl cation
beta-amyrin
-
-
r
5.4.99.B2
(S)-2,3-oxidosqualene
Malus domestica
via dammarenyl cation
lupeol
-
-
r
5.4.99.B2
(S)-2,3-oxidosqualene
Malus domestica
via dammarenyl cation
alpha-amyrin
-
-
r
5.4.99.34
(3S)-2,3-epoxy-2,3-dihydrosqualene
Malus domestica
-
germanicol
-
-
r
5.4.99.34
additional information
Malus domestica
although the MdOSC4 gene was expressed in the skins of all apple varieties under investigation, no germanicol or derivatives were detected at the metabolite level
?
-
-
-
5.4.99.39
(S)-2,3-oxidosqualene
Malus domestica
via dammarenyl cation
beta-amyrin
-
-
r
5.4.99.41
(S)-2,3-oxidosqualene
Malus domestica
via dammarenyl cation, low activity
beta-amyrin
-
-
r
5.4.99.41
(S)-2,3-oxidosqualene
Malus domestica
via dammarenyl cation
lupeol
-
-
r
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.126
additional information
gene expression pattern analysis of CYP716A175 in 20 apple cultivars
Malus domestica
-
5.4.99.B2
epidermis
-
Malus domestica
-
5.4.99.B2
fruit
-
Malus domestica
-
5.4.99.34
fruit
-
Malus domestica
-
5.4.99.34
additional information
although the MdOSC4 gene was expressed in the skins of all apple varieties under investigation, no germanicol or derivatives were detected at the metabolite level
Malus domestica
-
5.4.99.34
plant epidermis
-
Malus domestica
-
5.4.99.39
epidermis
-
Malus domestica
-
5.4.99.39
fruit
-
Malus domestica
-
5.4.99.41
epidermis
-
Malus domestica
-
5.4.99.41
fruit
-
Malus domestica
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.126
alpha-amyrin + 3 O2 + 3 NADPH + 3 H+
overall reaction
748772
Malus domestica
ursolate + 3 NADP+ + 4 H2O
-
-
-
?
1.14.14.126
beta-amyrin + 3 O2 + 3 NADPH + 3 H+
overall reaction
748772
Malus domestica
oleanolate + 3 NADP+ + 4 H2O
-
-
-
?
1.14.14.126
germanicol + 3 O2 + 3 NADPH + 3 H+
overall reaction
748772
Malus domestica
morolate + 3 NADP+ + 4 H2O
-
-
-
?
1.14.14.126
lupeol + 3 O2 + 3 NADPH + 3 H+
overall reaction
748772
Malus domestica
betulinate + 3 NADP+ + 4 H2O
-
-
-
?
1.14.14.126
additional information
CYP716A175 catalyses the C-28 oxidation of alpha-amyrin, beta-amyrin, lupeol and germanicol, producing ursolic acid, oleanolic acid, betulinic acid, and putatively morolic acid. CYP716A175 catalyses the three-step oxidation of triterpene backbones at the C-28 position
748772
Malus domestica
?
-
-
-
-
1.14.14.126
additional information
CYP716A175 catalyses the three-step oxidation of triterpene backbones at the C-28 position. CYP716A175 has a similar oxidizing capacity for the triterpene scaffold alpha-amyrin, beta-amyrin, and lupeol but not for germanicol
748772
Malus domestica
?
-
-
-
-
5.4.99.B2
(S)-2,3-oxidosqualene
via dammarenyl cation
748772
Malus domestica
beta-amyrin
-
-
-
r
5.4.99.B2
(S)-2,3-oxidosqualene
via dammarenyl cation
748772
Malus domestica
lupeol
-
-
-
r
5.4.99.B2
(S)-2,3-oxidosqualene
via dammarenyl cation
748772
Malus domestica
alpha-amyrin
-
-
-
r
5.4.99.B2
additional information
MdOSC1 is a mixed amyrin synthase, with a 5:1 ratio of alpha-amyrin to beta-amyrin. The alpha-amyrin to beta-amyrin to lupeol production ratio for MdOSC1 is 85:13:2
748772
Malus domestica
?
-
-
-
-
5.4.99.34
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
748772
Malus domestica
germanicol
-
-
-
r
5.4.99.34
(S)-2,3-oxidosqualene
via dammarenyl cation, low activity
748772
Malus domestica
beta-amyrin
-
-
-
r
5.4.99.34
(S)-2,3-oxidosqualene
via dammarenyl cation, low activity
748772
Malus domestica
lupeol
-
-
-
r
5.4.99.34
additional information
although the MdOSC4 gene was expressed in the skins of all apple varieties under investigation, no germanicol or derivatives were detected at the metabolite level
748772
Malus domestica
?
-
-
-
-
5.4.99.34
additional information
gene MdOSC4 encodes a multifunctional oxidosqualene cyclase producing an oleanane-type triterpene, putatively identified as germanicol, as well as beta-amyrin and lupeol, in the proportion 82:14:4
748772
Malus domestica
?
-
-
-
-
5.4.99.39
(S)-2,3-oxidosqualene
via dammarenyl cation
748772
Malus domestica
beta-amyrin
-
-
-
r
5.4.99.39
additional information
MdOSC1 is a mixed amyrin synthase, with a 5:1 ratio of alpha-amyrin to beta-amyrin. The alpha-amyrin to beta-amyrin to lupeol production ratio for MdOSC1 from (S)-2,3-oxidosqualene is 85:13:2
748772
Malus domestica
?
-
-
-
-
5.4.99.41
(S)-2,3-oxidosqualene
via dammarenyl cation, low activity
748772
Malus domestica
beta-amyrin
-
-
-
r
5.4.99.41
(S)-2,3-oxidosqualene
via dammarenyl cation
748772
Malus domestica
lupeol
-
-
-
r
5.4.99.41
additional information
MdOSC5 cyclizes 2,3-oxidosqualene into lupeol and beta-amyrin at a ratio of 95:5
748772
Malus domestica
?
-
-
-
-
General Information
EC Number
General Information
Commentary
Organism
1.14.14.126
evolution
CYP716A175 is clearly grouped with the CYP716A subfamily. Expression patterns of triterpene-related genes in different apple cultivars, overview
Malus domestica
5.4.99.B2
metabolism
analysis of biosynthetic pathway of triterpenic acids in apple fruits, overview; MdOSC1 and MdOSC5 are key genes in apple fruit triterpene biosynthesis, analysis of biosynthetic pathway of triterpenic acids in apple, overview. The gene expression of MdOSC1 is linked to the concentrations of ursolic and oleanolic acid
Malus domestica
5.4.99.B2
additional information
analysis of triterpenes from apple fruits in a collection of 20 contrasting apple cultivars, expression patterns of triterpene-related genes in different apple cultivars, overview; analysis of triterpenes from apple fruits in a collection of 20 contrasting apple cultivars, expression patterns of triterpene-related genes in different apple cultivars, overview
Malus domestica
5.4.99.B2
physiological function
the enzyme is involved in the biosynthesis of pentacyclic triterpenes, which, in apple, account for 32-70% of the epicuticular waxes, depending on the cultivar; the enzyme is involved in the biosynthesis of pentacyclic triterpenes, which, in apple, account for 32-70% of the epicuticular waxes, depending on the cultivar
Malus domestica
5.4.99.34
evolution
MdOSC4 contains the highly conserved DCTAE motif, which is implicated in substrate binding, and six repeats of the QW motifs, typical of the triterpene synthase superfamily, sequence comparisons and phylogenetic analysis
Malus domestica
5.4.99.34
metabolism
analysis of biosynthetic pathway of triterpenic acids in apple fruits, overview
Malus domestica
5.4.99.34
additional information
analysis of triterpenes from apple fruits in a collection of 20 contrasting apple cultivars, expression patterns of triterpene-related genes in different apple cultivars, overview
Malus domestica
5.4.99.34
physiological function
the enzyme is involved in the biosynthesis of pentacyclic triterpenes, which, in apple, account for 32-70% of the epicuticular waxes, depending on the cultivar
Malus domestica
5.4.99.39
metabolism
MdOSC1 and MdOSC5 are key genes in apple fruit triterpene biosynthesis, analysis of biosynthetic pathway of triterpenic acids in apple, overview. The gene expression of MdOSC1 is linked to the concentrations of ursolic and oleanolic acid
Malus domestica
5.4.99.39
additional information
analysis of triterpenes from apple fruits in a collection of 20 contrasting apple cultivars, expression patterns of triterpene-related genes in different apple cultivars, overview
Malus domestica
5.4.99.39
physiological function
the enzyme is involved in the biosynthesis of pentacyclic triterpenes, which, in apple, account for 32-70% of the epicuticular waxes, depending on the cultivar
Malus domestica
5.4.99.41
evolution
enzyme MdOSC5 contain the highly conserved DCTAE motif, which is implicated in substrate binding, and six repeats of the QW motifs, typical of the triterpene synthase superfamily. MdOSC5 is located in linkage group LG17, sequence comparisons and phylogenetic analysis
Malus domestica
5.4.99.41
metabolism
MdOSC1 and MdOSC5 are key genes in apple fruit triterpene biosynthesis, analysis of biosynthetic pathway of triterpenic acids in apple, overview. The gene expression of MdOSC1 is linked to the concentrations of ursolic and oleanolic acid
Malus domestica
5.4.99.41
additional information
analysis of triterpenes from apple fruits in a collection of 20 contrasting apple cultivars, expression patterns of triterpene-related genes in different apple cultivars, overview
Malus domestica
5.4.99.41
physiological function
the enzyme is involved in the biosynthesis of pentacyclic triterpenes, which, in apple, account for 32-70% of the epicuticular waxes, depending on the cultivar
Malus domestica
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.14.126
evolution
CYP716A175 is clearly grouped with the CYP716A subfamily. Expression patterns of triterpene-related genes in different apple cultivars, overview
Malus domestica
5.4.99.B2
metabolism
MdOSC1 and MdOSC5 are key genes in apple fruit triterpene biosynthesis, analysis of biosynthetic pathway of triterpenic acids in apple, overview. The gene expression of MdOSC1 is linked to the concentrations of ursolic and oleanolic acid
Malus domestica
5.4.99.B2
metabolism
analysis of biosynthetic pathway of triterpenic acids in apple fruits, overview
Malus domestica
5.4.99.B2
additional information
analysis of triterpenes from apple fruits in a collection of 20 contrasting apple cultivars, expression patterns of triterpene-related genes in different apple cultivars, overview
Malus domestica
5.4.99.B2
physiological function
the enzyme is involved in the biosynthesis of pentacyclic triterpenes, which, in apple, account for 32-70% of the epicuticular waxes, depending on the cultivar
Malus domestica
5.4.99.34
evolution
MdOSC4 contains the highly conserved DCTAE motif, which is implicated in substrate binding, and six repeats of the QW motifs, typical of the triterpene synthase superfamily, sequence comparisons and phylogenetic analysis
Malus domestica
5.4.99.34
metabolism
analysis of biosynthetic pathway of triterpenic acids in apple fruits, overview
Malus domestica
5.4.99.34
additional information
analysis of triterpenes from apple fruits in a collection of 20 contrasting apple cultivars, expression patterns of triterpene-related genes in different apple cultivars, overview
Malus domestica
5.4.99.34
physiological function
the enzyme is involved in the biosynthesis of pentacyclic triterpenes, which, in apple, account for 32-70% of the epicuticular waxes, depending on the cultivar
Malus domestica
5.4.99.39
metabolism
MdOSC1 and MdOSC5 are key genes in apple fruit triterpene biosynthesis, analysis of biosynthetic pathway of triterpenic acids in apple, overview. The gene expression of MdOSC1 is linked to the concentrations of ursolic and oleanolic acid
Malus domestica
5.4.99.39
additional information
analysis of triterpenes from apple fruits in a collection of 20 contrasting apple cultivars, expression patterns of triterpene-related genes in different apple cultivars, overview
Malus domestica
5.4.99.39
physiological function
the enzyme is involved in the biosynthesis of pentacyclic triterpenes, which, in apple, account for 32-70% of the epicuticular waxes, depending on the cultivar
Malus domestica
5.4.99.41
evolution
enzyme MdOSC5 contain the highly conserved DCTAE motif, which is implicated in substrate binding, and six repeats of the QW motifs, typical of the triterpene synthase superfamily. MdOSC5 is located in linkage group LG17, sequence comparisons and phylogenetic analysis
Malus domestica
5.4.99.41
metabolism
MdOSC1 and MdOSC5 are key genes in apple fruit triterpene biosynthesis, analysis of biosynthetic pathway of triterpenic acids in apple, overview. The gene expression of MdOSC1 is linked to the concentrations of ursolic and oleanolic acid
Malus domestica
5.4.99.41
additional information
analysis of triterpenes from apple fruits in a collection of 20 contrasting apple cultivars, expression patterns of triterpene-related genes in different apple cultivars, overview
Malus domestica
5.4.99.41
physiological function
the enzyme is involved in the biosynthesis of pentacyclic triterpenes, which, in apple, account for 32-70% of the epicuticular waxes, depending on the cultivar
Malus domestica