EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.99.8 | on the base of callus cells, enzyme CAS recombinant expression in Panax notoginseng cells using Agrobacterium tumefaciens strain EHA105 for transfection, coexpression with farnesyl diphosphate synthase (FPS). Plasmid pHellsgate-CAS is transformed into the wild-type cells, and pCAMBIA1300S-FPS is then introduced into the pHellsgate-CAS transformed cells. Quantitative real-time RT-PCR enzyme expression analysis | Panax notoginseng |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.4.99.8 | additional information | RNA interference (RNAi) of the cycloartenol synthase (CAS) gene and simultanous recombinant expression of farnesyl diphosphate synthase (FPS) gene in Panax notoginseng cells leads to both higher expression levels of FPS and lower expression levels of CAS compared to the wild-type cells. Transgenic cell lines provide a higher accumulation of total triterpene saponins, and a lower amount of phytosterols compared to wild-type cells. Overexpression of FPS can break the rate-limiting reaction catalyzed by FPS in the triterpene saponins biosynthetic pathway, and inhibition of CAS expression can decrease the synthesis metabolic flux of the phytosterol branch. Thus, more precursors flow in the direction of triterpene synthesis, and ultimately promote the accumulation of Panax notoginseng saponins. Silencing and overexpression of key enzyme genes simultaneously is more effective than just manipulating one gene in the regulation of saponin biosynthesis | Panax notoginseng |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.8 | (3S)-2,3-epoxy-2,3-dihydrosqualene | Panax notoginseng | - |
cycloartenol | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.8 | Panax notoginseng | A8UHA2 | collected from Wenshan, Yunnan Province, China | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
5.4.99.8 | callus culture | - |
Panax notoginseng | - |
5.4.99.8 | cell culture | - |
Panax notoginseng | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.8 | (3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Panax notoginseng | cycloartenol | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.8 | CAS | - |
Panax notoginseng |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.4.99.8 | malfunction | inhibition of CAS expression, e.g. by RNAi, can decrease the synthesis metabolic flux of the phytosterol branch | Panax notoginseng |
5.4.99.8 | metabolism | enzyme CAS catalyzes the conversion of 2,3-oxidosqualene to cycloartenol which is ultimately used to synthesize phytosterols. Although CAS does not participate in the biosynthesis of triterpene saponins, it competes with dammarenedion-II synthase (DS) for the same precursor (2,3-oxidosqualene). The 2,3-oxidosqualene is the common precursor of triterpene saponins and phytosterols | Panax notoginseng |