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Literature summary extracted from

  • Peek, J.; Roman, J.; Moran, G.R.; Christendat, D.
    Structurally diverse dehydroshikimate dehydratase variants participate in microbial quinate catabolism (2017), Mol. Microbiol., 103, 39-54 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.118 gene asbF, sequence comparisons and phylogenetic analysis Bacillus anthracis
4.2.1.118 gene quiC1, sequence comparisons and phylogenetic analysis Pseudomonas aeruginosa
4.2.1.118 gene quiC1, sequence comparisons and phylogenetic analysis, plasmid-based expression of Pseudomonas putida QuiC1 in the Pseudomonas aeruginosa quiC1 knockout strain, recombinant expression of wild-type and mutant enzymes Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.118 E356A site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme Pseudomonas putida
4.2.1.118 H168A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Pseudomonas putida
4.2.1.118 additional information quiC1 transposon quiC1 gene knockout in strain PAO1, recombinant plasmid-based expression of gene quiC1 from Pseudomonas putida QuiC1 in the Pseudomonas aeruginosa quiC1 knockout strain Pseudomonas aeruginosa
4.2.1.118 additional information the isolated N-terminal domain shows about 1/3 of the wild-type enzyme activity Pseudomonas putida
4.2.1.118 R207A site-directed mutagenesis, the mutant shows moderately reduced activity compared to the wild-type enzyme Pseudomonas putida
4.2.1.118 R210A site-directed mutagenesis, inactive mutant Pseudomonas putida
4.2.1.118 S206A site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.118 0.134
-
3-dehydro-shikimate pH 7.5, temperature not specified in the publication, recombinant mutant H168A Pseudomonas putida
4.2.1.118 0.205
-
3-dehydro-shikimate pH 7.5, temperature not specified in the publication, recombinant isolated N-terminal domain of the enzyme Pseudomonas putida
4.2.1.118 0.331
-
3-dehydro-shikimate pH 7.5, temperature not specified in the publication, recombinant wild-type enzyme Pseudomonas putida
4.2.1.118 0.429
-
3-dehydro-shikimate pH 7.5, temperature not specified in the publication, recombinant mutant E356A Pseudomonas putida
4.2.1.118 2.745
-
3-dehydro-shikimate pH 7.5, temperature not specified in the publication, recombinant mutant S206A Pseudomonas putida
4.2.1.118 5.311
-
3-dehydro-shikimate pH 7.5, temperature not specified in the publication, recombinant mutant R207A Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.118 Co2+ required Pseudomonas putida
4.2.1.118 Mg2+ enzyme bound Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.118 3-dehydro-shikimate Pseudomonas aeruginosa
-
protocatechuate + H2O
-
?
4.2.1.118 3-dehydro-shikimate Pseudomonas putida
-
protocatechuate + H2O
-
?
4.2.1.118 3-dehydro-shikimate Bacillus anthracis
-
protocatechuate + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.118 Bacillus anthracis Q81RQ4
-
-
4.2.1.118 Pseudomonas aeruginosa
-
-
-
4.2.1.118 Pseudomonas putida A0A379KX44
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.118 3-dehydro-shikimate
-
Pseudomonas aeruginosa protocatechuate + H2O
-
?
4.2.1.118 3-dehydro-shikimate
-
Pseudomonas putida protocatechuate + H2O
-
?
4.2.1.118 3-dehydro-shikimate
-
Bacillus anthracis protocatechuate + H2O
-
?

Subunits

EC Number Subunits Comment Organism
4.2.1.118 More three-dimensional structure comparisons, superimposition of the QuiC1 N-terminal (DSD) domain with Bacillus anthracis AsbF and structure comparisons with Pseudomonas fluorescens 4-hydroxyphenylpyruvate dioxygenase (HPPD), overview Pseudomonas putida
4.2.1.118 More three-dimensional structure comparisons, superimposition of the QuiC1 N-terminal (DSD) domain with Bacillus anthracis AsbF, and structure comparisons with Pseudomonas fluorescens 4-hydroxyphenylpyruvate dioxygenase (HPPD), overview Pseudomonas aeruginosa
4.2.1.118 More three-dimensional structure comparisons, superimposition of the QuiC1 N-terminal (DSD) domain with Bacillus anthracis AsbF, and structure comparisons with Pseudomonas fluorescens 4-hydroxyphenylpyruvate dioxygenase (HPPD), overview Bacillus anthracis

Synonyms

EC Number Synonyms Comment Organism
4.2.1.118 AsbF
-
Bacillus anthracis
4.2.1.118 dehydroshikimate dehydratase
-
Pseudomonas aeruginosa
4.2.1.118 dehydroshikimate dehydratase
-
Pseudomonas putida
4.2.1.118 dehydroshikimate dehydratase
-
Bacillus anthracis
4.2.1.118 DSD
-
Pseudomonas aeruginosa
4.2.1.118 DSD
-
Pseudomonas putida
4.2.1.118 DSD
-
Bacillus anthracis
4.2.1.118 Qui1
-
Pseudomonas aeruginosa
4.2.1.118 Qui1
-
Pseudomonas putida
4.2.1.118 quiC1
-
Pseudomonas aeruginosa
4.2.1.118 quiC1
-
Pseudomonas putida
4.2.1.118 quiC_1
-
Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.118 7.7
-
3-dehydro-shikimate pH 7.5, temperature not specified in the publication, recombinant mutant H168A Pseudomonas putida
4.2.1.118 61.2
-
3-dehydro-shikimate pH 7.5, temperature not specified in the publication, recombinant isolated N-terminal domain of the enzyme Pseudomonas putida
4.2.1.118 99.1
-
3-dehydro-shikimate pH 7.5, temperature not specified in the publication, recombinant mutant R207A Pseudomonas putida
4.2.1.118 138.2
-
3-dehydro-shikimate pH 7.5, temperature not specified in the publication, recombinant mutant E356A Pseudomonas putida
4.2.1.118 148.1
-
3-dehydro-shikimate pH 7.5, temperature not specified in the publication, recombinant mutant S206A Pseudomonas putida
4.2.1.118 163.6
-
3-dehydro-shikimate pH 7.5, temperature not specified in the publication, recombinant wild-type enzyme Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.118 7.5
-
assay at Pseudomonas putida

General Information

EC Number General Information Comment Organism
4.2.1.118 evolution the QuiC1 protein is not related by sequence with previously identified DSDs from the Gram-negative genus, Acinetobacter, but instead shows limited sequence identity in its N-terminal half with fungal DSDs Pseudomonas aeruginosa
4.2.1.118 evolution the QuiC1 protein is not related by sequence with previously identified DSDs from the Gram-negative genus, Acinetobacter, but instead shows limited sequence identity in its N-terminal half with fungal DSDs Pseudomonas putida
4.2.1.118 additional information enzyme structure-function analysis, overview Pseudomonas aeruginosa
4.2.1.118 additional information superimposition of the active sites of the Pseudomonas putida QuiC1 N-terminal domain and Bacillus anthracis AsbF in complex with protocatechuate. AsbF residues occupying the same position as QuiC1 Arg207 and Arg210 are not observed. Enzyme structure-function analysis, overview Pseudomonas putida
4.2.1.118 additional information superimposition of the active sites of the Pseudomonas putida QuiC1 N-terminal domain and Bacillus anthracis AsbF in complex with protocatechuate. AsbF residues occupying the same position as QuiC1 Arg207 and Arg210 are not observed. Enzyme structure-function analysis, overview Bacillus anthracis
4.2.1.118 physiological function enzyme Qui1 is important for growth on either quinate or shikimate Pseudomonas aeruginosa
4.2.1.118 physiological function enzyme Qui1 is important for growth on either quinate or shikimate Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.118 18.7
-
3-dehydro-shikimate pH 7.5, temperature not specified in the publication, recombinant mutant R207A Pseudomonas putida
4.2.1.118 53.9
-
3-dehydro-shikimate pH 7.5, temperature not specified in the publication, recombinant mutant S206A Pseudomonas putida
4.2.1.118 57.5
-
3-dehydro-shikimate pH 7.5, temperature not specified in the publication, recombinant mutant H168A Pseudomonas putida
4.2.1.118 298.5
-
3-dehydro-shikimate pH 7.5, temperature not specified in the publication, recombinant isolated N-terminal domain of the enzyme Pseudomonas putida
4.2.1.118 322.2
-
3-dehydro-shikimate pH 7.5, temperature not specified in the publication, recombinant mutant E356A Pseudomonas putida
4.2.1.118 494.3
-
3-dehydro-shikimate pH 7.5, temperature not specified in the publication, recombinant wild-type enzyme Pseudomonas putida