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Literature summary extracted from

  • Lalsiamthara, J.; Lee, J.H.
    Immuno-profiles of BALB/c mice inoculated with Salmonella vector delivering B-cell mitogen hydroxyproline epimerase (2018), Mol. Immunol., 95, 114-121 .
    View publication on PubMed

Application

EC Number Application Comment Organism
5.1.1.8 medicine PrpA can be used as a protein adjuvant in a live Salmonella delivery system, in order to increase humoral responses effectively without major interference on the cell mediated immunity Brucella abortus
5.1.1.8 molecular biology PrpA can be used as a protein adjuvant in a live Salmonella delivery system, in order to increase humoral responses effectively without major interference on the cell mediated immunity Brucella abortus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.8 gene prpA, live attenuated Salmonella typhimurium (ST) vector constitutively expressing heterologous Brucella abortus PrpA protein (ST-PrpA) is inoculated in BALB/c mice, recombinant expression of His-tagged Brucella abortus PrpA in Escherichia coli strain BL21 Brucella abortus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.8 trans-4-hydroxy-L-proline Brucella abortus
-
 cis-4-hydroxy-D-proline
-
 r
5.1.1.8 trans-4-hydroxy-L-proline Brucella abortus 2308
-
 cis-4-hydroxy-D-proline
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.8 Brucella abortus Q2YLF3
-
-
5.1.1.8 Brucella abortus 2308 Q2YLF3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.8 recombinnat His-tagged Brucella abortus PrpA from Escherichia coli strain BL21 by nickel affinity chromatography Brucella abortus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.8 trans-4-hydroxy-L-proline
-
 Brucella abortus cis-4-hydroxy-D-proline
-
 r
5.1.1.8 trans-4-hydroxy-L-proline
-
 Brucella abortus 2308 cis-4-hydroxy-D-proline
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.1.1.8 4-hydroxyproline 2-epimerase
-
 Brucella abortus
5.1.1.8 BaHyPRE
-
 Brucella abortus
5.1.1.8 Hydroxyproline epimerase
-
 Brucella abortus
5.1.1.8 PrpA
-
 Brucella abortus

Expression

EC Number Organism Comment Expression
5.1.1.8 Brucella abortus live attenuated Salmonella typhimurium (ST) vector constitutively expressing heterologous Brucella abortus S19 PrpA protein (ST-PrpA) is inoculated in BALB/c mice. No aggravation of morbidity is observed upon mice inoculation of ST-PrpA. Immunized mice show significantly quicker anti-Salmonella IgG responses as compared to ST only immunization, and an increase interleukin-4 production evident in in vitro pulsed mice splenocytes, splenocytes proliferative profiles and lymphocyte population evaluation, overview up

General Information

EC Number General Information Comment Organism
5.1.1.8 evolution 4-hydroxyproline 2-epimerase is a member of proline racemase family Brucella abortus
5.1.1.8 physiological function the enzyme 4-hydroxyproline 2-epimerase reversibly catalyse the interconversion of trans-4-hydroxy-L-proline and cis-4-hydroxy-D-proline. The Brucella proline racemase (PrpA) or hydroxyproline epimerase (BaHyPRE) also exhibit B-cell mitogenic properties thereby causing B-lymphocytes polyclonal activation and increase secretion of interleukin (IL)-10. Importance of PrpA in brucellosis Brucella abortus