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Literature summary extracted from
- Contribution of a low-barrier hydrogen bond to catalysis is not significant in ketosteroid isomerase (2015), Mol. Cells, 38, 409-415 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.3.1 | expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.3.3.1 | mutant enzyme Y30F/Y55F/Y115F/D38N KSI complexed with equilenin, hanging drop vapor diffusion method, using 0.1 M sodium acetate, pH 4.5, 0.6 M ammonium acetate, and 30% PEG 4000 | Pseudomonas putida |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.3.3.1 | Y115F | the mutant shows about 68% activity compared to the wild type enzyme | Pseudomonas putida |
| 5.3.3.1 | Y14F | the mutant shows almost no activity compared to the wild type enzyme | Pseudomonas putida |
| 5.3.3.1 | Y30F | the mutant shows about 84% activity compared to the wild type enzyme | Pseudomonas putida |
| 5.3.3.1 | Y30F/Y55F | the mutant shows about 50% activity compared to the wild type enzyme | Pseudomonas putida |
| 5.3.3.1 | Y30F/Y55F/Y115F | the mutant shows about 57% activity compared to the wild type enzyme | Pseudomonas putida |
| 5.3.3.1 | Y55F | the mutant shows about 17% activity compared to the wild type enzyme | Pseudomonas putida |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.3.1 | androstenolone | - |
Pseudomonas putida |  |
| 5.3.3.1 | equilenin | - |
Pseudomonas putida | |
| 5.3.3.1 | estrone | - |
Pseudomonas putida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.3.1 | 0.0171 | - |
5-androstene-3,17-dione | mutant enzyme Y14F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
| 5.3.3.1 | 0.023 | - |
5-androstene-3,17-dione | mutant enzyme Y55F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
| 5.3.3.1 | 0.0417 | - |
5-androstene-3,17-dione | mutant enzyme Y115F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
| 5.3.3.1 | 0.0499 | - |
5-androstene-3,17-dione | wild type enzyme, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
| 5.3.3.1 | 0.0502 | - |
5-androstene-3,17-dione | mutant enzyme Y30F/Y55F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
| 5.3.3.1 | 0.0552 | - |
5-androstene-3,17-dione | mutant enzyme Y30F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
| 5.3.3.1 | 0.0773 | - |
5-androstene-3,17-dione | mutant enzyme Y30F/Y55F/Y115F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.3.1 | Pseudomonas putida | P07445 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.3.1 | 5-Androstene-3,17-dione | - |
Pseudomonas putida | 4-Androstene-3,17-dione | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.3.1 | DELTA5-3-ketosteroid isomerase | - |
Pseudomonas putida |
| 5.3.3.1 | KSI | - |
Pseudomonas putida |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.3.1 | 13.3 | - |
5-androstene-3,17-dione | mutant enzyme Y14F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
| 5.3.3.1 | 3510 | - |
5-androstene-3,17-dione | mutant enzyme Y55F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
| 5.3.3.1 | 10700 | - |
5-androstene-3,17-dione | mutant enzyme Y30F/Y55F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
| 5.3.3.1 | 12200 | - |
5-androstene-3,17-dione | mutant enzyme Y30F/Y55F/Y115F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
| 5.3.3.1 | 14500 | - |
5-androstene-3,17-dione | mutant enzyme Y115F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
| 5.3.3.1 | 17800 | - |
5-androstene-3,17-dione | mutant enzyme Y30F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
| 5.3.3.1 | 21000 | - |
5-androstene-3,17-dione | wild type enzyme, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.3.1 | 780 | - |
5-androstene-3,17-dione | mutant enzyme Y14F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
| 5.3.3.1 | 150000 | - |
5-androstene-3,17-dione | mutant enzyme Y55F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
| 5.3.3.1 | 160000 | - |
5-androstene-3,17-dione | mutant enzyme Y30F/Y55F/Y115F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
| 5.3.3.1 | 210000 | - |
5-androstene-3,17-dione | mutant enzyme Y30F/Y55F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
| 5.3.3.1 | 330000 | - |
5-androstene-3,17-dione | mutant enzyme Y30F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
| 5.3.3.1 | 350000 | - |
5-androstene-3,17-dione | mutant enzyme Y115F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
| 5.3.3.1 | 430000 | - |
5-androstene-3,17-dione | wild type enzyme, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
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