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Literature summary extracted from

  • Liu, S.; Yu, H.; Liu, Y.; Liu, X.; Zhang, Y.; Bu, C.; Yuan, S.; Chen, Z.; Xie, G.; Li, W.; Xu, B.; Yang, J.; He, L.; Jin, T.; Xiong, Y.; Sun, L.; Liu, X.; Han, C.; Cheng, Z.; Liang, J.; Shang, Y.
    Chromodomain protein CDYL acts as a crotonyl-CoA hydratase to regulate histone crotonylation and spermatogenesis (2017), Mol. Cell, 67, 853-866.e5 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.150 additional information generation of Cdyl transgenic mice by microinjection of a Cdyl construct into the pro-nuclei of fertilized oocytes, derived from intercross of C57BL/6 3 CBA F1 mice Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.150 additional information
-
 additional information steady-state kinetics of the CDYL-catalyzed hydratation reaction compared to mitochondrial metabolic enzyme enoyl-CoA hydratase (ECH, EC 4.2.1.17) as a positive control Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.150 mitochondrion
-
 Homo sapiens 5739
-
4.2.1.150 mitochondrion
-
 Mus musculus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.150 crotonyl-CoA + H2O Homo sapiens
-
 3-hydroxybutyryl-CoA
-
 r
4.2.1.150 crotonyl-CoA + H2O Mus musculus
-
 3-hydroxybutyryl-CoA
-
 r
4.2.1.150 crotonyl-CoA + H2O Mus musculus C57BL/6
-
 3-hydroxybutyryl-CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.150 Homo sapiens Q9Y232
-
-
4.2.1.150 Mus musculus Q9WTK2
-
-
4.2.1.150 Mus musculus C57BL/6 Q9WTK2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.150 HeLa cell
-
 Homo sapiens
-
4.2.1.150 spermatid
-
 Homo sapiens
-
4.2.1.150 spermatid
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.150 crotonyl-CoA + H2O
-
 Homo sapiens 3-hydroxybutyryl-CoA
-
 r
4.2.1.150 crotonyl-CoA + H2O
-
 Mus musculus 3-hydroxybutyryl-CoA
-
 r
4.2.1.150 crotonyl-CoA + H2O
-
 Mus musculus C57BL/6 3-hydroxybutyryl-CoA
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.150 CDYL
-
 Homo sapiens
4.2.1.150 CDYL
-
 Mus musculus
4.2.1.150 chromodomain Y-like protein
-
 Homo sapiens
4.2.1.150 chromodomain Y-like protein
-
 Mus musculus
4.2.1.150 crotonyl-CoA hydratase
-
 Homo sapiens
4.2.1.150 crotonyl-CoA hydratase
-
 Mus musculus
4.2.1.150 More cf. EC 4.2.1.17 Homo sapiens
4.2.1.150 More cf. EC 4.2.1.17 Mus musculus

General Information

EC Number General Information Comment Organism
4.2.1.150 evolution the C-terminal CoAP domain of the CDY family proteins including CDYL has a three-dimensional structure closely resembling enoyl-CoA hydratase, which catalyzes the hydratation of 2-trans-enoyl-CoA into beta-hydroxyacyl-CoA in mitochondria during beta-oxidation of fatty acids Homo sapiens
4.2.1.150 evolution the C-terminal CoAP domain of the CDY family proteins including CDYL has a three-dimensional structure closely resembling enoyl-CoA hydratase, which catalyzes the hydratation of 2-trans-enoyl-CoA into beta-hydroxyacyl-CoA in mitochondria during beta-oxidation of fatty acids Mus musculus
4.2.1.150 malfunction Cdyl knockout mice are embryonically lethal or died shortly after birth with significantly reduced levels of histone Kcr in elongating spermatids from Cdyl transgenic mice compared to that from wild-type mice, although the level of histone acetylation is comparable Mus musculus
4.2.1.150 malfunction the levels of total histone Kcr and H2BK12cr on the promoter of known CDYL target genes BDNF, NEUROD1, SCG10, and MYT1 increase significantly in CDYL-KO HeLa cells, whereas the regional level of H3K27me3 in these cells decreases, expression patterns, overview Homo sapiens
4.2.1.150 additional information both the chromodomain and CoAP domain of CDYL are required for its negative regulation of histone Kcr Homo sapiens
4.2.1.150 additional information both the chromodomain and CoAP domain of CDYL are required for its negative regulation of histone Kcr Mus musculus
4.2.1.150 physiological function the chromodomain Y-like protein CDYL acts as a crotonyl-CoA hydratase to negatively regulate histone crotonylation. The chromodomain Y-like transcription corepressor CDYL negatively regulates histone Kcr by acting as a crotonyl-CoA hydratase to convert crotonyl-CoA to beta-hydroxybutyryl-CoA. This activity is intrinsically linked to the transcription repression function of CDYL and is implemented in reactivation of sex chromosome-linked genes and histone replacement during spermatogenesis. The negative regulation of histone Kcr by CDYL is intrinsically linked to its transcription repression activity and functionally implemented in the reactivation of sex chromosome-linked genes in round spermatids and genome-wide histone replacement in elongating spermatids Mus musculus
4.2.1.150 physiological function the chromodomain Y-like protein CDYL acts as a crotonyl-CoA hydratase to negatively regulate histone crotonylation. The chromodomain Y-like transcription corepressor CDYL negatively regulates histone Kcr by acting as a crotonyl-CoA hydratase to convert crotonyl-CoA to beta-hydroxybutyryl-CoA. This activity is intrinsically linked to the transcription repression function of CDYL and is implemented in reactivation of sex chromosome-linked genes and histone replacement during spermatogenesis. The negative regulation of histone Kcr by CDYL is intrinsically linked to its transcription repression activity and functionally implemented in the reactivation of sex chromosome-linked genes in round spermatids and genome-wide histone replacement in elongating spermatids. Cdyl regulates the expression of sex chromosome-linked escaped genes in postmeiotic spermatogenic cells by mainly influencing histone Kcr on the gene promoters. The enzyme is important in the physiology of male reproduction and the mechanism of the spermatogenic failure in AZFc (azoospermia factor c)-deleted infertile men Homo sapiens