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Literature summary extracted from

  • Sim, G.; Yang, S.; Kim, B.; Ahn, J.
    Bacterial synthesis of N-hydroxycinnamoyl phenethylamines and tyramines (2015), Microb. Cell Fact., 14, 162 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.53 gene PDC, recombinant expression in Escherichia coli strain HP-2, coexpression with genes TDC, TAL, SHT, and THT Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.53 additional information genes PDC, TDC, TAL, SHT, and THT are introduced into engineered Escherichia coli strain HP-2 to synthesize N-(p-coumaroyl) phenethylamine and N-(p-coumaroyl) tyramine, method evaluation and optimization, overview. p-Coumaric acid is synthesized from tyrosine by TAL and phenethylamine is synthesized from phenylalanine by PDC. Therefore, the intracellular concentrations of both tyrosine and phenylalanine are critical for the final yield of N-(p-coumaroyl) phenethylamine Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.53 L-phenylalanine Pseudomonas putida
-
phenylethylamine + CO2
-
?
4.1.1.53 L-phenylalanine Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440
-
phenylethylamine + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.53 Pseudomonas putida Q88JU5
-
-
4.1.1.53 Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 Q88JU5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.53 L-phenylalanine
-
Pseudomonas putida phenylethylamine + CO2
-
?
4.1.1.53 L-phenylalanine
-
Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 phenylethylamine + CO2
-
?
4.1.1.53 additional information enzyme PDC from Pseudomonas putida is active on L-amino acids and shows higher substrate specificity for phenylalanine than for tyrosine Pseudomonas putida ?
-
?
4.1.1.53 additional information enzyme PDC from Pseudomonas putida is active on L-amino acids and shows higher substrate specificity for phenylalanine than for tyrosine Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.53 More cf. EC 4.1.1.28 Pseudomonas putida
4.1.1.53 PDC
-
Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.53 pyridoxal 5'-phosphate
-
Pseudomonas putida