Any feedback?
Literature summary extracted from
- Production of functionalized oligo-isoprenoids by enzymatic cleavage of rubber (2017), Microb. Biotechnol., 10, 1426-1433 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.13.11.87 | synthesis | production of functionalized oligo-isoprenoids by enzymatic cleavage of rubber. A method for the preparation of reactive oligo-isoprenoids that can be used to convert polyisoprene latex or rubber waste materials into value-added molecules, biofuels, polyurethanes or other polymers. Emulsions of polyisoprene (latex) are treated with RoxAXsp, RoxBXsp, LcpK30 or with combinations of the three proteins. The cleavage products are purified by solvent extraction and FPLC separation. All products have the same general structure with terminal functions (CHO-CH2- and -CH2-COCH3) but differ in the number of intact isoprene units in between | Streptomyces sp. K30 |
| 1.13.11.87 | synthesis | production of functionalized oligo-isoprenoids by enzymatic cleavage of rubber. A method for the preparation of reactive oligo-isoprenoids that can be used to convert polyisoprene latex or rubber waste materials into value-added molecules, biofuels, polyurethanes or other polymers. Emulsions of polyisoprene (latex) are treated with RoxAXsp, RoxBXsp, LcpK30 or with combinations of the three proteins. The cleavage products are purified by solvent extraction and FPLC separation. All products have the same general structure with terminal functions (CHO-CH2- and -CH2-COCH3) but differ in the number of intact isoprene units in between | Xanthomonas sp. 35Y |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.13.11.87 | expression in Escherichia coli | Streptomyces sp. K30 |
| 1.13.11.87 | expression in Escherichia coli | Xanthomonas sp. 35Y |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.85 | 70000 | - |
gel filtration | Steroidobacter cummioxidans |
| 1.13.11.87 | 40000 | - |
gel filtration | Xanthomonas sp. 35Y |
| 1.13.11.87 | 70000 | - |
gel filtration | Streptomyces sp. K30 |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.85 | cis-1,4-polyisoprene + n O2 | Steroidobacter cummioxidans | - |
n (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? |  |
| 1.13.11.85 | cis-1,4-polyisoprene + n O2 | Steroidobacter cummioxidans 35Y | - |
n (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? | |
| 1.13.11.87 | cis-1,4-polyisoprene + n O2 | Streptomyces sp. K30 | the enzyme produces a mixture of oligo-isoprenoids (C20, C25, C30 and higher oligo-isoprenoids) | ? | - |
? | |
| 1.13.11.87 | cis-1,4-polyisoprene + n O2 | Xanthomonas sp. 35Y | the enzyme produces a mixture of oligo-isoprenoids (C20, C25, C30 and higher oligo-isoprenoids) | ? | - |
? | |
| 1.13.11.87 | cis-1,4-polyisoprene + O2 | Streptomyces sp. K30 | - |
? | - |
? | |
| 1.13.11.87 | cis-1,4-polyisoprene + O2 | Xanthomonas sp. 35Y | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.85 | Steroidobacter cummioxidans | - |
- |
- |
| 1.13.11.85 | Steroidobacter cummioxidans 35Y | - |
- |
- |
| 1.13.11.87 | Streptomyces sp. K30 | - |
- |
- |
| 1.13.11.87 | Streptomyces sp. K30 | Q3L8N0 | - |
- |
| 1.13.11.87 | Xanthomonas sp. 35Y | - |
- |
- |
| 1.13.11.87 | Xanthomonas sp. 35Y | A0A1S6Q8F9 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.87 | - |
Streptomyces sp. K30 |
| 1.13.11.87 | - |
Xanthomonas sp. 35Y |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.85 | cis-1,4-polyisoprene + n O2 | - |
Steroidobacter cummioxidans | n (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? | |
| 1.13.11.85 | cis-1,4-polyisoprene + n O2 | - |
Steroidobacter cummioxidans 35Y | n (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? | |
| 1.13.11.87 | cis-1,4-polyisoprene + n O2 | the enzyme produces a mixture of oligo-isoprenoids (C20, C25, C30 and higher oligo-isoprenoids) | Streptomyces sp. K30 | ? | - |
? | |
| 1.13.11.87 | cis-1,4-polyisoprene + n O2 | the enzyme produces a mixture of oligo-isoprenoids (C20, C25, C30 and higher oligo-isoprenoids) | Xanthomonas sp. 35Y | ? | - |
? | |
| 1.13.11.87 | cis-1,4-polyisoprene + O2 | - |
Streptomyces sp. K30 | ? | - |
? | |
| 1.13.11.87 | cis-1,4-polyisoprene + O2 | - |
Xanthomonas sp. 35Y | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.11.85 | roxA | - |
Steroidobacter cummioxidans |
| 1.13.11.85 | rubber oxygenase | - |
Steroidobacter cummioxidans |
| 1.13.11.87 | latex-clearing protein | - |
Streptomyces sp. K30 |
| 1.13.11.87 | Lcp | - |
Streptomyces sp. K30 |
| 1.13.11.87 | LcpK30 | - |
Streptomyces sp. K30 |
| 1.13.11.87 | RoxB | - |
Xanthomonas sp. 35Y |
| 1.13.11.87 | rubber oxygenase | - |
Xanthomonas sp. 35Y |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.85 | heme | c-type diheme dioxygenase | Steroidobacter cummioxidans | |
| 1.13.11.87 | heme | - |
Xanthomonas sp. 35Y | |
| 1.13.11.87 | heme | c-type diheme dioxygenase | Streptomyces sp. K30 | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
