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Literature summary extracted from

  • Gas-Pascual, E.; Simonovik, B.; Heintz, D.; Bergdoll, M.; Schaller, H.; Bach, T.J.
    Inhibition of cycloartenol synthase (CAS) function in tobacco BY-2 cell suspensions a proteomic analysis (2015), Lipids, 50, 773-784 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.8 gene CAS, recombinant expression in TBY-2 cells, usage of Agrobacterium tumefaciens strain LBA4404 hyper-virulent strain and transformation into BY-2 cell suspensions via co-culture, quantitative reverse transcriptase-mediated real-time PCR enzyme expression analysis. After 6 days of treatment, the expression of NtCAS1 increases 2.5fold in the BY-2 cells Nicotiana tabacum

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.8 Ro 48-8071 a potent inhibitor of oxidosqualene cyclization, the site of RO 48-8071 inhibition is CAS Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.8 (3S)-2,3-epoxy-2,3-dihydrosqualene Nicotiana tabacum
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cycloartenol
-
r

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.8 Nicotiana tabacum A0A0A1ER86 cv. BY-2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.99.8 cell suspension culture
-
Nicotiana tabacum
-
5.4.99.8 leaf
-
Nicotiana tabacum
-
5.4.99.8 TBY-2 cell
-
Nicotiana tabacum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.8 (3S)-2,3-epoxy-2,3-dihydrosqualene
-
Nicotiana tabacum cycloartenol
-
r

Synonyms

EC Number Synonyms Comment Organism
5.4.99.8 NtCAS
-
Nicotiana tabacum

Expression

EC Number Organism Comment Expression
5.4.99.8 Nicotiana tabacum inhibitor RO 48-8071 leads to downregulation of gene CAS in cells. CAS gene expression is partially blocked by using an inducible artificial microRNA with limited effectiveness down

General Information

EC Number General Information Comment Organism
5.4.99.8 malfunction in vivo application of inhibitor RO 48-8071 TBY-2 cells in short-term treatments (24 h) results in accumulation of oxidosqualene with no changes in the final sterol products. Long-term treatments (6 days) induces downregulation in gene expression not only of CAS but also of the SMT2 gene coding sterol methyltransferase 2 (EC 2.1.1.41) explaining some of the increase in 24-methyl sterols at the expense of the 24-ethyl sterols stigmasterol and sitosterol Nicotiana tabacum
5.4.99.8 metabolism building blocks for plant sterol biosynthesis, involving enzyme CAS, are provided by the mevalonate (MVA) pathway, overview Nicotiana tabacum