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Literature summary extracted from

  • Xu, S.X.; Qin, X.; Liu, B.; Zhang, D.Q.; Zhang, W.; Wu, K.; Zhang, Y.H.
    An acidic pectin lyase from Aspergillus niger with favourable efficiency in fruit juice clarification (2015), Lett. Appl. Microbiol., 60, 181-187 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.2.10 food industry pectin lyase PNL-ZJ5A can effectively decrease the viscosity and improve the yield of apple juice and light transmittance Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.10 expression in Pichia pastoris Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.10 EDTA 10 mM, more than 50% inhibition Aspergillus niger
4.2.2.10 Li+ 10 mM, 40% inhibition Aspergillus niger
4.2.2.10 Ni2+ 10 mM, more than 50% inhibition Aspergillus niger
4.2.2.10 SDS 10 mM, more than 50% inhibition Aspergillus niger
4.2.2.10 Zn2+ 10 mM, more than 50% inhibition Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.10 additional information
-
 citrus pectin Km value 0.66 mg/ml, pH 5.0, 40°C Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.10 Aspergillus niger
-
-
-
4.2.2.10 Aspergillus niger ZJ5
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.10 26.85
-
 pH 5.0, 43°C Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.10 citrus pectin
-
 Aspergillus niger ?
-
 ?
4.2.2.10 citrus pectin
-
 Aspergillus niger ZJ5 ?
-
 ?
4.2.2.10 pectin with 70% methyl esterification 44% of the activity with citrus pectin Aspergillus niger ?
-
 ?
4.2.2.10 pectin with 70% methyl esterification 44% of the activity with citrus pectin Aspergillus niger ZJ5 ?
-
 ?
4.2.2.10 pectin with 85% methyl esterification 118% of the activity with citrus pectin Aspergillus niger ?
-
 ?
4.2.2.10 pectin with 85% methyl esterification 118% of the activity with citrus pectin Aspergillus niger ZJ5 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.10 pnl-zj5a
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.10 43
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.10 43
-
 1 h, pH 5.0, 77% residual activity Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.10 5
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.10 4.3 5.5
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.10 3 6 1 h, 37°C, more than 70% residual activity Aspergillus niger