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Literature summary extracted from

  • Wang, T.; Dai, K.; Liu, H.; Jia, S.; Wang, R.
    Cloning and expression of a trehalose synthase from Pseudomonas putida KT2440 in Bacillus subtilis W800N for the production of trehalose (2014), J. Pure Appl. Microbiol., 8, 1687-1692 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.16 gene treSB, DNA and amino acid sequence determination and analysis, recombinant expression in Bacillus subtilis strain W800N, recombinant expression of His6-tagged enzyme in Escherichia coli strain DH5alpha Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.16 Ca2+
-
 Pseudomonas putida
5.4.99.16 Zn2+
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.99.16 K+ activates Pseudomonas putida
5.4.99.16 Mg2+ activates Pseudomonas putida
5.4.99.16 Na+ activates Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.16 maltose Pseudomonas putida
-
 alpha,alpha-trehalose
-
 r
5.4.99.16 maltose Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440
-
 alpha,alpha-trehalose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.16 Pseudomonas putida Q88FN0
-
-
5.4.99.16 Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 Q88FN0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.16 recombinant His6-tagged enzyme by nickel affinity chromatography Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.16 maltose
-
 Pseudomonas putida alpha,alpha-trehalose
-
 r
5.4.99.16 maltose
-
 Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 alpha,alpha-trehalose
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.4.99.16 Trehalose synthase
-
 Pseudomonas putida
5.4.99.16 TreS
-
 Pseudomonas putida
5.4.99.16 treSB
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.16 25
-
 recombinant His6-tagged enzyme Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.16 8
-
 recombinant His6-tagged enzyme Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.4.99.16 6 9 purified recombinant His6-tagged enzyme, stable at Pseudomonas putida