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Literature summary extracted from

  • Yoshitomi, K.; Taniguchi, S.; Tanaka, K.; Uji, Y.; Akimitsu, K.; Gomi, K.
    Rice terpene synthase 24 (OsTPS24) encodes a jasmonate-responsive monoterpene synthase that produces an antibacterial gamma-terpinene against rice pathogen (2016), J. Plant Physiol., 191, 120-126 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.114 gene OsTPS24, DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis, functional recombinant expression of His-tagged recombinant TPS24 lacking the putative chloroplast transit sequence in Escherichia coli, expression of GFP-tagged enzyme in Oryza sativa protoplasts chloroplasts Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.114 chloroplast the enzyme has a chloroplast transit sequence Oryza sativa 9507
-
4.2.3.114 additional information in general, monoterpene synthases contain a transit peptide for translocation to plastids and sesquiterpene synthases are localized in the cytoplasm Oryza sativa
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.114 geranyl diphosphate Oryza sativa
-
 gamma-terpinene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.114 Oryza sativa
-
 cv. Nipponbare
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.114 recombinant His-tagged recombinant TPS24 lacking the putative chloroplast transit sequence from Escherichia coli by nickel affinity chromatography and dialysis Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.114 leaf
-
 Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.114 geranyl diphosphate
-
 Oryza sativa gamma-terpinene + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.114 ? x * 67700, about, sequence calculation Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
4.2.3.114 Os04g27790 locus name Oryza sativa
4.2.3.114 OsTPS24
-
 Oryza sativa
4.2.3.114 terpene synthase 24
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.114 30
-
 assay at Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.114 7.5
-
 assay at Oryza sativa

Expression

EC Number Organism Comment Expression
4.2.3.114 Oryza sativa the enzyme encoded by gene OsTPS24 is highly induced about 20fold by jasmonate, expression pattern, overview up

General Information

EC Number General Information Comment Organism
4.2.3.114 physiological function enzyme OsTPS24 is localized in chloroplasts and produced the monoterpene, gamma-terpinene. The amount of gamma-terpinene increased after jasmonate treatment. gamma-Terpinene has significant antibacterial activity against Xanthomonas oryzae pv. oryzae (Xoo), but it does not show significant antifungal activity against Magnaporthe oryzae. The antibacterial activity of gamma-terpinene against Xoo is caused by damage to bacterial cell membranes. These results suggest that gamma-terpinene plays an important role in jasmonate-induced resistance against Xoo, and that it functions as an antibacterial compound in rice plants Oryza sativa