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Literature summary extracted from
- Structure and function of benzylsuccinate synthase and related fumarate-adding glycyl radical enzymes (2016), J. Mol. Microbiol. Biotechnol., 26, 29-44 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.99.11 | gene order of all known bss operons is bssDCAB, starting with the gene for the activating enzyme and followed by those for the gamma-, alpha- and beta-subunits, phylogenetic analysis | Desulfosarcina cetonica |
| 4.1.99.11 | gene order of all known bss operons is bssDCAB, starting with the gene for the activating enzyme and followed by those for the gamma-, alpha- and beta-subunits, phylogenetic analysis | Thauera aromatica |
| 4.1.99.11 | gene order of all known bss operons is bssDCAB, starting with the gene for the activating enzyme and followed by those for the gamma-, alpha- and beta-subunits, phylogenetic analysis | Azoarcus sp. CIB |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.99.11 | toluene + fumarate | Desulfosarcina cetonica | - |
benzylsuccinate | - |
? |  |
| 4.1.99.11 | toluene + fumarate | Thauera aromatica | - |
benzylsuccinate | - |
? | |
| 4.1.99.11 | toluene + fumarate | Azoarcus sp. CIB | - |
benzylsuccinate | - |
? | |
| 4.1.99.11 | toluene + fumarate | Thauera aromatica K172 | - |
benzylsuccinate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.99.11 | Azoarcus sp. CIB | I6LHT8 | alpha-subunit | - |
| 4.1.99.11 | Desulfosarcina cetonica | - |
- |
- |
| 4.1.99.11 | Thauera aromatica | O87943 AND O87944 AND O87942 | alpha-, beta-, and gamma-subunit | - |
| 4.1.99.11 | Thauera aromatica K172 | O87943 AND O87944 AND O87942 | alpha-, beta-, and gamma-subunit | - |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 4.1.99.11 | benzylsuccinate = toluene + fumarate | DFT model of the reaction mechanism of BSS, mechanistic modeling | Desulfosarcina cetonica | |
| 4.1.99.11 | benzylsuccinate = toluene + fumarate | DFT model of the reaction mechanism of BSS, mechanistic modeling | Thauera aromatica | |
| 4.1.99.11 | benzylsuccinate = toluene + fumarate | DFT model of the reaction mechanism of BSS, mechanistic modeling | Azoarcus sp. CIB |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.99.11 | toluene + fumarate | - |
Desulfosarcina cetonica | benzylsuccinate | - |
? | |
| 4.1.99.11 | toluene + fumarate | - |
Thauera aromatica | benzylsuccinate | - |
? | |
| 4.1.99.11 | toluene + fumarate | - |
Azoarcus sp. CIB | benzylsuccinate | - |
? | |
| 4.1.99.11 | toluene + fumarate | - |
Thauera aromatica K172 | benzylsuccinate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.99.11 | More | the enzyme has a trimeric structure of alpha-, beta- and gamma subunits. Structure comparisons of BSS enzymes and BSS-like fumarate-adding enzymes (FAEs) | Desulfosarcina cetonica |
| 4.1.99.11 | More | the enzyme has a trimeric structure of alpha-, beta- and gamma subunits. Structure comparisons of BSS enzymes and BSS-like fumarate-adding enzymes (FAEs) | Thauera aromatica |
| 4.1.99.11 | More | the enzyme has a trimeric structure of alpha-, beta- and gamma subunits. Structure comparisons of BSS enzymes and BSS-like fumarate-adding enzymes (FAEs) | Azoarcus sp. CIB |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.99.11 | BSS | - |
Desulfosarcina cetonica |
| 4.1.99.11 | BSS | - |
Thauera aromatica |
| 4.1.99.11 | BSS | - |
Azoarcus sp. CIB |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.1.99.11 | evolution | enzyme BSS is a member of the glycyl radical enzyme (GRE) family. Structure and function of benzylsuccinate synthase and related fumarate-adding glycyl radical enzymes, phylogenetic analysis, overview | Desulfosarcina cetonica |
| 4.1.99.11 | evolution | enzyme BSS is a member of the glycyl radical enzyme (GRE) family. Structure and function of benzylsuccinate synthase and related fumarate-adding glycyl radical enzymes, phylogenetic analysis, overview | Thauera aromatica |
| 4.1.99.11 | evolution | enzyme BSS is a member of the glycyl radical enzyme (GRE) family. Structure and function of benzylsuccinate synthase and related fumarate-adding glycyl radical enzymes, phylogenetic analysis, overview | Azoarcus sp. CIB |
| 4.1.99.11 | additional information | enzyme structure and structure-based mechanistic models, overview. The QM cluster model contains whole or fragmented side chains of nine active site amino acids surrounding the bound substrates, Glu189, Tyr197, Ser199, Ile384, Leu391, Leu492, Cys493, Arg508, and Val709 | Desulfosarcina cetonica |
| 4.1.99.11 | additional information | enzyme structure and structure-based mechanistic models, overview. The QM cluster model contains whole or fragmented side chains of nine active site amino acids surrounding the bound substrates, Glu189, Tyr197, Ser199, Ile384, Leu391, Leu492, Cys493, Arg508, and Val709 | Thauera aromatica |
| 4.1.99.11 | additional information | enzyme structure and structure-based mechanistic models, overview. The QM cluster model contains whole or fragmented side chains of nine active site amino acids surrounding the bound substrates, Glu189, Tyr197, Ser199, Ile384, Leu391, Leu492, Cys493, Arg508, and Val709 | Azoarcus sp. CIB |
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