EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.17 | expressed in Escherichia coli BL21 Star (DE3) | Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.17 | 0.14 | - |
ATP | 37°C, pH 7.5 | Thermotoga maritima | |
2.7.1.17 | 0.22 | - |
D-xylulose | 37°C, pH 7.5 | Thermotoga maritima | |
2.7.1.17 | 0.7 | - |
ATP | 60°C, pH 7.5 | Thermotoga maritima | |
2.7.1.17 | 1.24 | - |
D-xylulose | 60°C, pH 7.5 | Thermotoga maritima | |
2.7.1.17 | 17 | - |
(Polyphosphate)n | 37°C, pH 7.5 | Thermotoga maritima |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.17 | 60000 | - |
SDS-PAGE | Thermotoga maritima |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.17 | Thermotoga maritima | Q9WXX1 | - |
- |
2.7.1.17 | Thermotoga maritima DSM 3109 | Q9WXX1 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.17 | (polyphosphate)n + D-xylulose | activity with polyphosphate is 41 % of its specific activity on ATP | Thermotoga maritima | (polyphosphate)n-1 + D-xylulose 5-phosphate | - |
? | |
2.7.1.17 | (polyphosphate)n + D-xylulose | activity with polyphosphate is 41 % of its specific activity on ATP | Thermotoga maritima DSM 3109 | (polyphosphate)n-1 + D-xylulose 5-phosphate | - |
? | |
2.7.1.17 | ATP + D-xylulose | - |
Thermotoga maritima | ADP + D-xylulose 5-phosphate | - |
? | |
2.7.1.17 | ATP + D-xylulose | - |
Thermotoga maritima DSM 3109 | ADP + D-xylulose 5-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.17 | ? | x * 60000, SDS-PAGE | Thermotoga maritima |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.17 | ATP/polyphosphate xylulokinase | - |
Thermotoga maritima |
2.7.1.17 | TM0116 | - |
Thermotoga maritima |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.17 | 85 | - |
- |
Thermotoga maritima |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.17 | 65 | 95 | 65°C: about 60% of maximal activity, 95°: about 60% of maximal activity | Thermotoga maritima |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.17 | 60 | - |
degradation constants at 0.1 and 0.01 g/l xylulokinase at 60°C are 0.20/h and 0.35/h, respectively. The presence of 2 mM xylulose enhances the thermostability of 0.01 g/l xylulokinase, increasing degradation constants to 0.29/h | Thermotoga maritima |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.17 | 8.77 | - |
(Polyphosphate)n | 37°C, pH 7.5 | Thermotoga maritima | |
2.7.1.17 | 24 | - |
ATP | 37°C, pH 7.5 | Thermotoga maritima | |
2.7.1.17 | 26 | - |
D-xylulose | 37°C, pH 7.5 | Thermotoga maritima | |
2.7.1.17 | 78 | - |
D-xylulose | 60°C, pH 7.5 | Thermotoga maritima | |
2.7.1.17 | 83 | - |
ATP | 60°C, pH 7.5 | Thermotoga maritima |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.17 | 62 | - |
D-xylulose | 60°C, pH 7.5 | Thermotoga maritima | |
2.7.1.17 | 116 | - |
D-xylulose | 37°C, pH 7.5 | Thermotoga maritima | |
2.7.1.17 | 119 | - |
ATP | 60°C, pH 7.5 | Thermotoga maritima | |
2.7.1.17 | 171 | - |
ATP | 37°C, pH 7.5 | Thermotoga maritima | |
2.7.1.17 | 516 | - |
(Polyphosphate)n | 37°C, pH 7.5 | Thermotoga maritima |