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Literature summary extracted from

  • Oelschlaegel, M.; Richter, L.; Stuhr, A.; Hofmann, S.; Schloemann, M.
    Heterologous production of different styrene oxide isomerases for the highly efficient synthesis of phenylacetaldehyde (2017), J. Biotechnol., 252, 43-49 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.99.7 expressed in Escherichia coli BL21(DE3) cells Pseudomonas fluorescens
5.3.99.7 expressed in Escherichia coli BL21(DE3) cells Rhodococcus opacus
5.3.99.7 expressed in Escherichia coli BL21(DE3) cells Sphingopyxis fribergensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.99.7 styrene oxide Pseudomonas fluorescens
-
 phenylacetaldehyde
-
 ?
5.3.99.7 styrene oxide Rhodococcus opacus
-
 phenylacetaldehyde
-
 ?
5.3.99.7 styrene oxide Sphingopyxis fribergensis
-
 phenylacetaldehyde
-
 ?
5.3.99.7 styrene oxide Rhodococcus opacus 1CP
-
 phenylacetaldehyde
-
 ?
5.3.99.7 styrene oxide Pseudomonas fluorescens ST
-
 phenylacetaldehyde
-
 ?
5.3.99.7 styrene oxide Sphingopyxis fribergensis Kp5.2
-
 phenylacetaldehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.7 Pseudomonas fluorescens O06836
-
-
5.3.99.7 Pseudomonas fluorescens ST O06836
-
-
5.3.99.7 Rhodococcus opacus A0A076JZU4
-
-
5.3.99.7 Rhodococcus opacus 1CP A0A076JZU4
-
-
5.3.99.7 Sphingopyxis fribergensis A0A076K0M9
-
-
5.3.99.7 Sphingopyxis fribergensis Kp5.2 A0A076K0M9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.99.7 partial purification Pseudomonas fluorescens
5.3.99.7 partial purification Rhodococcus opacus
5.3.99.7 partial purification Sphingopyxis fribergensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.99.7 4.1
-
 crude cell extract, at pH 7.2 and 37°C Pseudomonas fluorescens
5.3.99.7 9.79
-
 after 2.4fold purification, at pH 7.2 and 37°C Pseudomonas fluorescens
5.3.99.7 32.2
-
 crude cell extract, at pH 7.2 and 37°C Sphingopyxis fribergensis
5.3.99.7 75.9
-
 crude cell extract, at pH 7.2 and 37°C Rhodococcus opacus
5.3.99.7 89.7
-
 after 2.8fold purification, at pH 7.2 and 37°C Sphingopyxis fribergensis
5.3.99.7 150
-
 after 2fold purification, at pH 7.2 and 37°C Rhodococcus opacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.7 styrene oxide
-
 Pseudomonas fluorescens phenylacetaldehyde
-
 ?
5.3.99.7 styrene oxide
-
 Rhodococcus opacus phenylacetaldehyde
-
 ?
5.3.99.7 styrene oxide
-
 Sphingopyxis fribergensis phenylacetaldehyde
-
 ?
5.3.99.7 styrene oxide
-
 Rhodococcus opacus 1CP phenylacetaldehyde
-
 ?
5.3.99.7 styrene oxide
-
 Pseudomonas fluorescens ST phenylacetaldehyde
-
 ?
5.3.99.7 styrene oxide
-
 Sphingopyxis fribergensis Kp5.2 phenylacetaldehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.99.7 SOI
-
 Pseudomonas fluorescens
5.3.99.7 SOI
-
 Rhodococcus opacus
5.3.99.7 SOI
-
 Sphingopyxis fribergensis
5.3.99.7 StyC
-
 Pseudomonas fluorescens
5.3.99.7 StyC
-
 Rhodococcus opacus
5.3.99.7 StyC
-
 Sphingopyxis fribergensis
5.3.99.7 styrene oxide isomerase
-
 Pseudomonas fluorescens
5.3.99.7 styrene oxide isomerase
-
 Rhodococcus opacus
5.3.99.7 styrene oxide isomerase
-
 Sphingopyxis fribergensis