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Literature summary extracted from

  • Kudou, D.; Yasuda, E.; Hirai, Y.; Tamura, T.; Inagaki, K.
    Molecular cloning and characterization of L-methionine gamma-lyase from Streptomyces avermitilis (2015), J. Biosci. Bioeng., 120, 380-383 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.11 expression in Escherichia coli Streptomyces avermitilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.11 0.2
-
L-cysteine pH 8.0, 37°C Streptomyces avermitilis
4.4.1.11 1.6
-
L-methionine pH 8.0, 37°C Streptomyces avermitilis
4.4.1.11 1.78
-
L-homocysteine pH 8.0, 37°C Streptomyces avermitilis

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.11 Streptomyces avermitilis Q826W3
-
-
4.4.1.11 Streptomyces avermitilis DSM 46492 Q826W3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.11 recombinant protein Streptomyces avermitilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.11 L-cysteine + H2O
-
Streptomyces avermitilis sulfide + NH3 + pyruvate
-
?
4.4.1.11 L-cysteine + H2O
-
Streptomyces avermitilis DSM 46492 sulfide + NH3 + pyruvate
-
?
4.4.1.11 L-homocysteine + H2O
-
Streptomyces avermitilis sulfide + NH3 + 2-oxobutanoate
-
?
4.4.1.11 L-homocysteine + H2O
-
Streptomyces avermitilis DSM 46492 sulfide + NH3 + 2-oxobutanoate
-
?
4.4.1.11 L-methionine + H2O
-
Streptomyces avermitilis methanethiol + NH3 + 2-oxobutanoate
-
?
4.4.1.11 L-methionine + H2O
-
Streptomyces avermitilis DSM 46492 methanethiol + NH3 + 2-oxobutanoate
-
?

Subunits

EC Number Subunits Comment Organism
4.4.1.11 ? x * 42994, calculated from sequence Streptomyces avermitilis

Synonyms

EC Number Synonyms Comment Organism
4.4.1.11 sav7062
-
Streptomyces avermitilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.11 0.03
-
L-cysteine pH 8.0, 37°C Streptomyces avermitilis
4.4.1.11 1.7
-
L-homocysteine pH 8.0, 37°C Streptomyces avermitilis
4.4.1.11 2.9
-
L-methionine pH 8.0, 37°C Streptomyces avermitilis

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.11 pyridoxal 5'-phosphate
-
Streptomyces avermitilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.4.1.11 0.15
-
L-cysteine pH 8.0, 37°C Streptomyces avermitilis
4.4.1.11 0.94
-
L-homocysteine pH 8.0, 37°C Streptomyces avermitilis
4.4.1.11 1.81
-
L-methionine pH 8.0, 37°C Streptomyces avermitilis