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Literature summary extracted from

  • Roth, H.; Tessmer, I.; Van Houten, B.; Kisker, C.
    Bax1 is a novel endonuclease Implications for archaeal nucleotide excision repair (2009), J. Biol. Chem., 284, 32272-32278 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.99.B6 Bax1 wild-type as well as Bax1 mutant proteins are expressed in Escherichia coli BL21 Thermoplasma acidophilum

Protein Variants

EC Number Protein Variants Comment Organism
3.1.99.B6 D130A impaired incision activity, 25-35% of wild-type activity Thermoplasma acidophilum
3.1.99.B6 D132A activity is reduced to less than 20% of the wild-type activity Thermoplasma acidophilum
3.1.99.B6 E124A activity is reduced to less than 20% of the wild-type activity Thermoplasma acidophilum
3.1.99.B6 E124A/E125A impaired incision activity, 25-35% of wild-type activity Thermoplasma acidophilum
3.1.99.B6 E125A slightly impaired in the incision assay, exerts more than 70% of activity Thermoplasma acidophilum
3.1.99.B6 E134A impaired incision activity, 25-35% of wild-type activity Thermoplasma acidophilum
3.1.99.B6 F116A slightly impaired in the incision assay, exerts more than 70% of activity Thermoplasma acidophilum
3.1.99.B6 L137E impaired incision activity, 25-35% of wild-type activity Thermoplasma acidophilum
3.1.99.B6 N153A slightly impaired in the incision assay, exerts more than 70% of activity Thermoplasma acidophilum
3.1.99.B6 N153A/E155A activity is reduced to less than 20% of the wild-type activity Thermoplasma acidophilum
3.1.99.B6 Y128A impaired incision activity, 25-35% of wild-type activity Thermoplasma acidophilum
3.1.99.B6 Y152A activity is reduced to less than 20% of the wild-type activity Thermoplasma acidophilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.99.B6 Ca2+
-
Thermoplasma acidophilum
3.1.99.B6 EDTA
-
Thermoplasma acidophilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.99.B6 Mg2+ dependent on Mg2+ Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
3.1.99.B6 Thermoplasma acidophilum Q9HJF2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.99.B6
-
Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.99.B6 DNA + H2O structure-specific endonuclease recognizing DNA containing a 3' overhang. The enzyme specifically incises DNA in the single-stranded region of the 3' overhang 4-6 nucleotides to the single-stranded DNA/double stranded DNA junction and thus is a structure-specific and not a sequence-specific endonuclease Thermoplasma acidophilum ?
-
?
3.1.99.B6 additional information no incision is detected in the presence of a 5x02 overhang, double-stranded DNA, or DNA containing few unpaired nucleotides forming a bubble Thermoplasma acidophilum ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.99.B6 Ta1017
-
Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.99.B6 45
-
assay at Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.99.B6 6.5
-
assay at Thermoplasma acidophilum

General Information

EC Number General Information Comment Organism
3.1.99.B6 metabolism the enzyme is involved in nucleotide excision repair, a DNA repair mechanism that is responsible for the removal of a vast diversity of bulky DNA damages Thermoplasma acidophilum