Literature summary extracted from
Roth, H.; Tessmer, I.; Van Houten, B.; Kisker, C.
Bax1 is a novel endonuclease Implications for archaeal nucleotide excision repair (2009), J. Biol. Chem., 284, 32272-32278 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.99.B6 |
Bax1 wild-type as well as Bax1 mutant proteins are expressed in Escherichia coli BL21 |
Thermoplasma acidophilum |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.1.99.B6 |
D130A |
impaired incision activity, 25-35% of wild-type activity |
Thermoplasma acidophilum |
3.1.99.B6 |
D132A |
activity is reduced to less than 20% of the wild-type activity |
Thermoplasma acidophilum |
3.1.99.B6 |
E124A |
activity is reduced to less than 20% of the wild-type activity |
Thermoplasma acidophilum |
3.1.99.B6 |
E124A/E125A |
impaired incision activity, 25-35% of wild-type activity |
Thermoplasma acidophilum |
3.1.99.B6 |
E125A |
slightly impaired in the incision assay, exerts more than 70% of activity |
Thermoplasma acidophilum |
3.1.99.B6 |
E134A |
impaired incision activity, 25-35% of wild-type activity |
Thermoplasma acidophilum |
3.1.99.B6 |
F116A |
slightly impaired in the incision assay, exerts more than 70% of activity |
Thermoplasma acidophilum |
3.1.99.B6 |
L137E |
impaired incision activity, 25-35% of wild-type activity |
Thermoplasma acidophilum |
3.1.99.B6 |
N153A |
slightly impaired in the incision assay, exerts more than 70% of activity |
Thermoplasma acidophilum |
3.1.99.B6 |
N153A/E155A |
activity is reduced to less than 20% of the wild-type activity |
Thermoplasma acidophilum |
3.1.99.B6 |
Y128A |
impaired incision activity, 25-35% of wild-type activity |
Thermoplasma acidophilum |
3.1.99.B6 |
Y152A |
activity is reduced to less than 20% of the wild-type activity |
Thermoplasma acidophilum |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.99.B6 |
Ca2+ |
- |
Thermoplasma acidophilum |
|
3.1.99.B6 |
EDTA |
- |
Thermoplasma acidophilum |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.99.B6 |
Mg2+ |
dependent on Mg2+ |
Thermoplasma acidophilum |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.99.B6 |
Thermoplasma acidophilum |
Q9HJF2 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.99.B6 |
- |
Thermoplasma acidophilum |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.99.B6 |
DNA + H2O |
structure-specific endonuclease recognizing DNA containing a 3' overhang. The enzyme specifically incises DNA in the single-stranded region of the 3' overhang 4-6 nucleotides to the single-stranded DNA/double stranded DNA junction and thus is a structure-specific and not a sequence-specific endonuclease |
Thermoplasma acidophilum |
? |
- |
? |
|
3.1.99.B6 |
additional information |
no incision is detected in the presence of a 5x02 overhang, double-stranded DNA, or DNA containing few unpaired nucleotides forming a bubble |
Thermoplasma acidophilum |
? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.99.B6 |
Ta1017 |
- |
Thermoplasma acidophilum |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.1.99.B6 |
45 |
- |
assay at |
Thermoplasma acidophilum |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.1.99.B6 |
6.5 |
- |
assay at |
Thermoplasma acidophilum |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
3.1.99.B6 |
metabolism |
the enzyme is involved in nucleotide excision repair, a DNA repair mechanism that is responsible for the removal of a vast diversity of bulky DNA damages |
Thermoplasma acidophilum |