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Literature summary extracted from

  • Zhang, J.; Zhang, Y.; Inouye, M.
    Thermotoga maritima MazG protein has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities (2003), J. Biol. Chem., 278, 21408-21414 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.1 expression in Escherichia coli Thermotoga maritima
3.6.1.9 expression in Escherichia coli Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.1 E41Q/E42Q significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increases in the pyrophosphatase activity Thermotoga maritima
3.6.1.1 E45Q significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increases in the pyrophosphatase activity Thermotoga maritima
3.6.1.1 E61Q significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increases in the pyrophosphatase activity Thermotoga maritima
3.6.1.1 K118A significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increases in the pyrophosphatase activity Thermotoga maritima
3.6.1.1 R97A/R98A significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increases in the pyrophosphatase activity Thermotoga maritima
3.6.1.9 E41Q/E42Q significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increase in the pyrophosphatase activity Thermotoga maritima
3.6.1.9 E45Q significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increase in the pyrophosphatase activity Thermotoga maritima
3.6.1.9 E61Q significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increase in the pyrophosphatase activity Thermotoga maritima
3.6.1.9 K118A significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increase in the pyrophosphatase activity Thermotoga maritima
3.6.1.9 R97A/R98A significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increase in the pyrophosphatase activity Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.1 alpha,beta-methyleneadenosine triphosphate cannot be hydrolyzed and blocks both NTPase and pyrophosphatase activities Thermotoga maritima
3.6.1.9 alpha,beta-methyleneadenosine triphosphate cannot be hydrolyzed and blocks both NTPase and pyrophosphatase activities Thermotoga maritima
3.6.1.9 diphosphate
-
Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.9 0.4
-
CTP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 1
-
ATP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 1
-
GTP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 1
-
dGTP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 1.1
-
UTP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 1.5
-
dTTP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 1.7
-
dCTP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 3.2
-
dATP pH 8.0, 70°C Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.1 29728
-
mass spectrometry Thermotoga maritima
3.6.1.9 29728
-
mass spectrometry Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Thermotoga maritima Q9X015
-
-
3.6.1.1 Thermotoga maritima DSM 3109 Q9X015
-
-
3.6.1.9 Thermotoga maritima Q9X015
-
-
3.6.1.9 Thermotoga maritima DSM 3109 Q9X015
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.1
-
Thermotoga maritima
3.6.1.9
-
Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 diphosphate + H2O
-
Thermotoga maritima 2 phosphate
-
?
3.6.1.1 diphosphate + H2O
-
Thermotoga maritima DSM 3109 2 phosphate
-
?
3.6.1.1 additional information the product of the TM0913 gene, has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities Thermotoga maritima ?
-
?
3.6.1.1 additional information the product of the TM0913 gene, has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities Thermotoga maritima DSM 3109 ?
-
?
3.6.1.9 ATP + H2O
-
Thermotoga maritima AMP + diphosphate
-
?
3.6.1.9 CTP + H2O
-
Thermotoga maritima CMP + diphosphate
-
?
3.6.1.9 dATP + H2O
-
Thermotoga maritima dAMP + diphosphate
-
?
3.6.1.9 dCTP + H2O
-
Thermotoga maritima dCMP + diphosphate
-
?
3.6.1.9 dCTP + H2O
-
Thermotoga maritima DSM 3109 dCMP + diphosphate
-
?
3.6.1.9 dGTP + H2O dGTP is the best substrate among the deoxyribonucleoside triphosphates Thermotoga maritima dGMP + diphosphate
-
?
3.6.1.9 dTTP + H2O
-
Thermotoga maritima dTMP + diphosphate
-
?
3.6.1.9 GTP + H2O GTP is the best substrate among the ribonucleoside triphosphates Thermotoga maritima GMP + diphosphate
-
?
3.6.1.9 additional information the product of the TM0913 gene, has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities Thermotoga maritima ?
-
?
3.6.1.9 additional information the product of the TM0913 gene, has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities Thermotoga maritima DSM 3109 ?
-
?
3.6.1.9 UTP + H2O
-
Thermotoga maritima UMP + diphosphate
-
?
3.6.1.9 UTP + H2O
-
Thermotoga maritima DSM 3109 UMP + diphosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.1 MazG
-
Thermotoga maritima
3.6.1.1 pyrophosphatase
-
Thermotoga maritima
3.6.1.1 TM0913
-
Thermotoga maritima
3.6.1.9 MazG
-
Thermotoga maritima
3.6.1.9 nucleoside triphosphate pyrophosphohydrolase
-
Thermotoga maritima
3.6.1.9 TM0913
-
Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.1 80
-
-
Thermotoga maritima
3.6.1.9 80
-
-
Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.6.1.1 40 90 40°C: about 50% of maximal activity, 90°C: about 95% of maximal activity Thermotoga maritima
3.6.1.9 50 90 50°C: about 45% of maximal activity, 90°C: about 95% of maximal activity Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.1 85
-
stable at Thermotoga maritima
3.6.1.9 85
-
stable at Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.9 0.11
-
CTP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 0.18
-
UTP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 0.197
-
ATP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 0.65
-
dTTP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 0.69
-
GTP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 0.94
-
dGTP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 0.99
-
dCTP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 1.12
-
dATP pH 8.0, 70°C Thermotoga maritima

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.9 0.16
-
UTP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 0.198
-
ATP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 0.298
-
CTP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 0.35
-
dATP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 0.44
-
dTTP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 0.57
-
dCTP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 0.68
-
GTP pH 8.0, 70°C Thermotoga maritima
3.6.1.9 0.94
-
dGTP pH 8.0, 70°C Thermotoga maritima