EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.1 | expression in Escherichia coli | Thermotoga maritima |
3.6.1.9 | expression in Escherichia coli | Thermotoga maritima |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.1.1 | E41Q/E42Q | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increases in the pyrophosphatase activity | Thermotoga maritima |
3.6.1.1 | E45Q | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increases in the pyrophosphatase activity | Thermotoga maritima |
3.6.1.1 | E61Q | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increases in the pyrophosphatase activity | Thermotoga maritima |
3.6.1.1 | K118A | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increases in the pyrophosphatase activity | Thermotoga maritima |
3.6.1.1 | R97A/R98A | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increases in the pyrophosphatase activity | Thermotoga maritima |
3.6.1.9 | E41Q/E42Q | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increase in the pyrophosphatase activity | Thermotoga maritima |
3.6.1.9 | E45Q | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increase in the pyrophosphatase activity | Thermotoga maritima |
3.6.1.9 | E61Q | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increase in the pyrophosphatase activity | Thermotoga maritima |
3.6.1.9 | K118A | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increase in the pyrophosphatase activity | Thermotoga maritima |
3.6.1.9 | R97A/R98A | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increase in the pyrophosphatase activity | Thermotoga maritima |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.1 | alpha,beta-methyleneadenosine triphosphate | cannot be hydrolyzed and blocks both NTPase and pyrophosphatase activities | Thermotoga maritima | |
3.6.1.9 | alpha,beta-methyleneadenosine triphosphate | cannot be hydrolyzed and blocks both NTPase and pyrophosphatase activities | Thermotoga maritima | |
3.6.1.9 | diphosphate | - |
Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.9 | 0.4 | - |
CTP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 1 | - |
ATP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 1 | - |
GTP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 1 | - |
dGTP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 1.1 | - |
UTP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 1.5 | - |
dTTP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 1.7 | - |
dCTP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 3.2 | - |
dATP | pH 8.0, 70°C | Thermotoga maritima |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.6.1.1 | 29728 | - |
mass spectrometry | Thermotoga maritima |
3.6.1.9 | 29728 | - |
mass spectrometry | Thermotoga maritima |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.1 | Thermotoga maritima | Q9X015 | - |
- |
3.6.1.1 | Thermotoga maritima DSM 3109 | Q9X015 | - |
- |
3.6.1.9 | Thermotoga maritima | Q9X015 | - |
- |
3.6.1.9 | Thermotoga maritima DSM 3109 | Q9X015 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.1 | - |
Thermotoga maritima |
3.6.1.9 | - |
Thermotoga maritima |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.1 | diphosphate + H2O | - |
Thermotoga maritima | 2 phosphate | - |
? | |
3.6.1.1 | diphosphate + H2O | - |
Thermotoga maritima DSM 3109 | 2 phosphate | - |
? | |
3.6.1.1 | additional information | the product of the TM0913 gene, has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities | Thermotoga maritima | ? | - |
? | |
3.6.1.1 | additional information | the product of the TM0913 gene, has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities | Thermotoga maritima DSM 3109 | ? | - |
? | |
3.6.1.9 | ATP + H2O | - |
Thermotoga maritima | AMP + diphosphate | - |
? | |
3.6.1.9 | CTP + H2O | - |
Thermotoga maritima | CMP + diphosphate | - |
? | |
3.6.1.9 | dATP + H2O | - |
Thermotoga maritima | dAMP + diphosphate | - |
? | |
3.6.1.9 | dCTP + H2O | - |
Thermotoga maritima | dCMP + diphosphate | - |
? | |
3.6.1.9 | dCTP + H2O | - |
Thermotoga maritima DSM 3109 | dCMP + diphosphate | - |
? | |
3.6.1.9 | dGTP + H2O | dGTP is the best substrate among the deoxyribonucleoside triphosphates | Thermotoga maritima | dGMP + diphosphate | - |
? | |
3.6.1.9 | dTTP + H2O | - |
Thermotoga maritima | dTMP + diphosphate | - |
? | |
3.6.1.9 | GTP + H2O | GTP is the best substrate among the ribonucleoside triphosphates | Thermotoga maritima | GMP + diphosphate | - |
? | |
3.6.1.9 | additional information | the product of the TM0913 gene, has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities | Thermotoga maritima | ? | - |
? | |
3.6.1.9 | additional information | the product of the TM0913 gene, has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities | Thermotoga maritima DSM 3109 | ? | - |
? | |
3.6.1.9 | UTP + H2O | - |
Thermotoga maritima | UMP + diphosphate | - |
? | |
3.6.1.9 | UTP + H2O | - |
Thermotoga maritima DSM 3109 | UMP + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.1 | MazG | - |
Thermotoga maritima |
3.6.1.1 | pyrophosphatase | - |
Thermotoga maritima |
3.6.1.1 | TM0913 | - |
Thermotoga maritima |
3.6.1.9 | MazG | - |
Thermotoga maritima |
3.6.1.9 | nucleoside triphosphate pyrophosphohydrolase | - |
Thermotoga maritima |
3.6.1.9 | TM0913 | - |
Thermotoga maritima |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.1 | 80 | - |
- |
Thermotoga maritima |
3.6.1.9 | 80 | - |
- |
Thermotoga maritima |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.1 | 40 | 90 | 40°C: about 50% of maximal activity, 90°C: about 95% of maximal activity | Thermotoga maritima |
3.6.1.9 | 50 | 90 | 50°C: about 45% of maximal activity, 90°C: about 95% of maximal activity | Thermotoga maritima |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.1 | 85 | - |
stable at | Thermotoga maritima |
3.6.1.9 | 85 | - |
stable at | Thermotoga maritima |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.9 | 0.11 | - |
CTP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 0.18 | - |
UTP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 0.197 | - |
ATP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 0.65 | - |
dTTP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 0.69 | - |
GTP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 0.94 | - |
dGTP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 0.99 | - |
dCTP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 1.12 | - |
dATP | pH 8.0, 70°C | Thermotoga maritima |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.9 | 0.16 | - |
UTP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 0.198 | - |
ATP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 0.298 | - |
CTP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 0.35 | - |
dATP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 0.44 | - |
dTTP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 0.57 | - |
dCTP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 0.68 | - |
GTP | pH 8.0, 70°C | Thermotoga maritima | |
3.6.1.9 | 0.94 | - |
dGTP | pH 8.0, 70°C | Thermotoga maritima |