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Literature summary extracted from

  • Sutter, J.M.; Taestensen, J.B.; Johnsen, U.; Soppa, J.; Schoenheit, P.
    Key enzymes of the semiphosphorylative Entner-Doudoroff pathway in the haloarchaeon Haloferax volcanii characterization of glucose dehydrogenase, gluconate dehydratase, and 2-keto-3-deoxy-6-phosphogluconate aldolase (2016), J. Bacteriol., 198, 2251-2262 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.14 expressed in Escherichia coli and Haloferax volcanii H1209 Haloferax volcanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.14 0.29
-
 2-dehydro-3-deoxy-6-phospho-D-gluconate at pH 7.0 and 42°C Haloferax volcanii
4.1.2.14 38
-
 D-glyceraldehyde 3-phosphate at pH 7.0 and 42°C Haloferax volcanii
4.2.1.39 3
-
 D-gluconate pH 7.5, 42°C Haloferax volcanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.14 K+ maximal activities at concentrations of between 1 and 1.5 M KCl Haloferax volcanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.14 267000
-
 gel filtration Haloferax volcanii
4.2.1.39 340000
-
 gel filtration Haloferax volcanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.14 2-dehydro-3-deoxy-6-phospho-D-gluconate Haloferax volcanii
-
 pyruvate + D-glyceraldehyde 3-phosphate
-
 r
4.1.2.14 2-dehydro-3-deoxy-6-phospho-D-gluconate Haloferax volcanii H26
-
 pyruvate + D-glyceraldehyde 3-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.14 Haloferax volcanii D4GV57
-
-
4.1.2.14 Haloferax volcanii H26 D4GV57
-
-
4.2.1.39 Haloferax volcanii D4GYD4
-
-
4.2.1.39 Haloferax volcanii DSM 3757 D4GYD4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.14 Ni-NTA column chromatography and Superdex 200 gel filtration Haloferax volcanii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.39 30
-
 pH 7.5, 42°C Haloferax volcanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.14 2-dehydro-3-deoxy-6-phospho-D-galactonate low activity Haloferax volcanii ?
-
 ?
4.1.2.14 2-dehydro-3-deoxy-6-phospho-D-galactonate low activity Haloferax volcanii H26 ?
-
 ?
4.1.2.14 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 Haloferax volcanii pyruvate + D-glyceraldehyde 3-phosphate
-
 r
4.1.2.14 2-dehydro-3-deoxy-6-phospho-D-gluconate 100% activity Haloferax volcanii pyruvate + D-glyceraldehyde 3-phosphate
-
 r
4.1.2.14 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 Haloferax volcanii H26 pyruvate + D-glyceraldehyde 3-phosphate
-
 r
4.1.2.14 2-dehydro-3-deoxy-6-phospho-D-gluconate 100% activity Haloferax volcanii H26 pyruvate + D-glyceraldehyde 3-phosphate
-
 r
4.1.2.14 2-dehydro-3-deoxy-D-gluconate about 1% activity compared to 2-dehydro-3-deoxy-6-phospho-D-gluconate Haloferax volcanii pyruvate + D-glyceraldehyde
-
 ?
4.1.2.14 2-dehydro-3-deoxy-D-gluconate about 1% activity compared to 2-dehydro-3-deoxy-6-phospho-D-gluconate Haloferax volcanii H26 pyruvate + D-glyceraldehyde
-
 ?
4.1.2.14 pyruvate + D-glyceraldehyde 3-phosphate
-
 Haloferax volcanii 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 r
4.1.2.14 pyruvate + D-glyceraldehyde 3-phosphate
-
 Haloferax volcanii H26 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 r
4.2.1.39 D-galactonate low activity Haloferax volcanii 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
4.2.1.39 D-galactonate low activity Haloferax volcanii DSM 3757 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
4.2.1.39 D-gluconate
-
 Haloferax volcanii 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?
4.2.1.39 D-gluconate
-
 Haloferax volcanii DSM 3757 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?
4.2.1.39 additional information the enzyme catalyzes the specific dehydration of gluconate and does not utilize galactonate at significant rates Haloferax volcanii ?
-
 ?
4.2.1.39 additional information the enzyme catalyzes the specific dehydration of gluconate and does not utilize galactonate at significant rates Haloferax volcanii DSM 3757 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.14 homododecamer 12 * 22400, calculated from amino acid sequence Haloferax volcanii
4.2.1.39 octamer 8 * 45800, calculated from sequence Haloferax volcanii

Synonyms

EC Number Synonyms Comment Organism
4.1.2.14 2-keto-3-deoxy-6-phosphogluconate aldolase
-
 Haloferax volcanii
4.1.2.14 KDPG aldolase
-
 Haloferax volcanii
4.1.2.14 KDPGA
-
 Haloferax volcanii
4.2.1.39 GAD
-
 Haloferax volcanii
4.2.1.39 HVO_1488
-
 Haloferax volcanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.14 7
-
-
 Haloferax volcanii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.14 5.5 9 50% activity between pH 5.5 and 9.0 Haloferax volcanii

General Information

EC Number General Information Comment Organism
4.1.2.14 metabolism the enzyme is essential for glucose degradation Haloferax volcanii
4.2.1.39 physiological function a knockout mutant of GAD loses the ability to grow on glucose. Following a prolonged incubation period, GAD is functionally replaced by xylonate dehydratase Haloferax volcanii