Literature summary extracted from
Li, Y.; Mortuza, R.; Milligan, D.L.; Tran, S.L.; Strych, U.; Cook, G.M.; Krause, K.L.
Investigation of the essentiality of glutamate racemase in Mycobacterium smegmatis (2014), J. Bacteriol., 196, 4239-4244 .
Application
EC Number |
Application |
Comment |
Organism |
---|
5.1.1.3 |
drug development |
enzyme MurI is essential and can serve as a target for structure-aided drug design |
Mycolicibacterium smegmatis |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.1.1.3 |
gene murI, the murI gene lies in an operon and overlaps MSMEG_4904 at its 5' end by 4 bp. It is closely followed by MSMEG_4902 at its 3' end |
Mycolicibacterium smegmatis |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
5.1.1.3 |
additional information |
generation of a DELTAmurI strain of Mycobacterium smegmatis, the deletion of the murI gene can be achieved only in minimal medium supplemented with D-glutamate. Gene murI deletion via the gene replacement vector, pKKYL02, temperature-sensitive vector propagation and allelic exchange mutagenesis. Phenotype of DELTAmurI mutant strains on solid minimal medium, DELTAmurI mutant cells display a morphology different from the wild-type cells with pear-shaped swellings at their polar ends, overview |
Mycolicibacterium smegmatis |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.1.1.3 |
Mycolicibacterium smegmatis |
A0R1X0 |
- |
- |
5.1.1.3 |
Mycolicibacterium smegmatis ATCC 700084 / mc(2)155 |
A0R1X0 |
- |
- |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
5.1.1.3 |
physiological function |
MurI is essential for growth, and glutamate racemase is the only source of D-glutamate for peptidoglycan synthesis in Mycobacterium smegmatis |
Mycolicibacterium smegmatis |