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Literature summary extracted from

  • Cech, D.; Wang, P.F.; Holler, T.P.; Woodard, R.W.
    Analysis of the arabinose-5-phosphate isomerase of Bacteroides fragilis provides insight into regulation of single-domain arabinose phosphate isomerases (2014), J. Bacteriol., 196, 2861-2868 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.13 cytidine 5'-monophospho-3-deoxy-D-manno-2-octulosonic acid final product of the 3-deoxy-D-manno-2-octulosonic acid biosynthesis pathway Bacteroides fragilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.13 0.485
-
 D-ribulose 5-phosphate pH 8.5, 37°C Bacteroides fragilis
5.3.1.13 34.5
-
 D-arabinose 5-phosphate pH 8.5, 37°C Bacteroides fragilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.13 additional information purified protein does not contain metal ions Bacteroides fragilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.13 85900
-
 gel fitration Bacteroides fragilis

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.13 Bacteroides fragilis Q5LIW1
-
-
5.3.1.13 Bacteroides fragilis DSM 2151 Q5LIW1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.13 D-Arabinose 5-phosphate
-
 Bacteroides fragilis D-Ribulose 5-phosphate
-
 r
5.3.1.13 D-Arabinose 5-phosphate
-
 Bacteroides fragilis DSM 2151 D-Ribulose 5-phosphate
-
 r
5.3.1.13 D-ribulose 5-phosphate
-
 Bacteroides fragilis D-arabinose 5-phosphate
-
 r
5.3.1.13 D-ribulose 5-phosphate
-
 Bacteroides fragilis DSM 2151 D-arabinose 5-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.1.13 tetramer 4 * 21000, SDS-PAGE, 4 * 21810, mass spectrometry Bacteroides fragilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.13 18.5
-
 D-ribulose 5-phosphate pH 8.5, 37°C Bacteroides fragilis
5.3.1.13 253
-
 D-arabinose 5-phosphate pH 8.5, 37°C Bacteroides fragilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.13 7.8 9 broad Bacteroides fragilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.1.13 0.0019
-
 cytidine 5'-monophospho-3-deoxy-D-manno-2-octulosonic acid pH 8.5, 37°C Bacteroides fragilis

General Information

EC Number General Information Comment Organism
5.3.1.13 physiological function expression in Escherichia coli complements an arabinose-5-phosphate isomerase deficient strain Bacteroides fragilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.13 7.3
-
 D-arabinose 5-phosphate pH 8.5, 37°C Bacteroides fragilis
5.3.1.13 38
-
 D-ribulose 5-phosphate pH 8.5, 37°C Bacteroides fragilis