Literature summary extracted from
Caulkins, B.; Young, R.; Kudla, R.; Yang, C.; Bittbauer, T.; Bastin, B.; Hilario, E.; Fan, L.; Marsella, M.; Dunn, M.; Mueller, L.
NMR crystallography of a carbanionic intermediate in tryptophan synthase chemical structure, tautomerization, and reaction specificity (2016), J. Am. Chem. Soc., 138, 15214-15226 .
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.2.1.20 |
in complex with inhibitor N-(4'-trifluoro-methoxybenzenesulfonyl)-2-amino-ethyl phosphate |
Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.2.1.20 |
N-(4'-trifluoro-methoxybenzenesulfonyl)-2-amino-ethyl phosphate |
tight binding analogue of 1-C-(indol-3-yl)glycerol 3-phosphate which binds to the alpha-subunit |
Salmonella enterica subsp. enterica serovar Typhimurium |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.2.1.20 |
Salmonella enterica subsp. enterica serovar Typhimurium |
P00929 |
- |
- |
4.2.1.20 |
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 |
P00929 |
- |
- |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.2.1.20 |
TrpA |
alpha-chain |
Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
4.2.1.20 |
pyridoxal 5'-phosphate |
residue Lys87 of the beta-subunit activates C4' of pyridoxal 5'-phosphate for substrate nucleophilic attack |
Salmonella enterica subsp. enterica serovar Typhimurium |
|
General Information
EC Number |
General Information |
Comment |
Organism |
---|
4.2.1.20 |
physiological function |
residue Lys87 of the beta subunit plays multiple catalytic roles, it bonds to the pyridoxal phosphate cofactor, activates C4' for nucleophilic attack via a protonated Schiff base nitrogen, and abstracts and returns protons to pyridoxal phosphate-bound substrates. The protonation state of the epsilon-amino group switches between protonated and neutral states as the beta-subunit undergoes conversion from one intermediate to the next during catalysis |
Salmonella enterica subsp. enterica serovar Typhimurium |