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Literature summary extracted from

  • Lutz, S.; Lichter, J.; Liu, L.
    Exploiting temperature-dependent substrate promiscuity for nucleoside analogue activation by thymidine kinase from Thermotoga maritima (2007), J. Am. Chem. Soc., 129, 8714-8715 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.21 heterologously overexpressed in Escherichia coli Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 0.0005
-
 thymidine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.00065
-
 2',3'-dideoxy-3'-azidothymidine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.001
-
 5-fluoro-2'-deoxyuridine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.001
-
 thymidine 82°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.0015
-
 2'-deoxyuridine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.0087
-
 2',3'-dideoxy-3'-azidothymidine 82°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.03
-
 2',3'-dideoxythymidine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.04
-
 ATP 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.075
-
 2',3'-dideoxy-2',3'-didehydrothymidine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.114
-
 dioxolane thymidine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.132
-
 2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.187
-
 ATP 82°C, pH not specified in the publication Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Thermotoga maritima Q9WYN2
-
-
2.7.1.21 Thermotoga maritima DSM 3109 Q9WYN2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + 2',3'-dideoxy-2',3'-didehydrothymidine
-
 Thermotoga maritima ADP + 2',3'-dideoxy-2',3'-didehydrothymidine 5'-phosphate
-
 ?
2.7.1.21 ATP + 2',3'-dideoxy-3'-azidothymidine
-
 Thermotoga maritima ADP + 2',3'-dideoxy-3'-azidothymidine 5'-phosphate
-
 ?
2.7.1.21 ATP + 2',3'-dideoxythymidine
-
 Thermotoga maritima ADP + 2',3'-dideoxythymidine 5'-phosphate
-
 ?
2.7.1.21 ATP + 2',3'-dideoxythymidine
-
 Thermotoga maritima DSM 3109 ADP + 2',3'-dideoxythymidine 5'-phosphate
-
 ?
2.7.1.21 ATP + 2'-deoxyuridine
-
 Thermotoga maritima ADP + 2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + 2'-deoxyuridine
-
 Thermotoga maritima DSM 3109 ADP + 2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + 2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil
-
 Thermotoga maritima ADP + 2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil 5'-phosphate
-
 ?
2.7.1.21 ATP + 5-fluoro-2'-deoxyuridine
-
 Thermotoga maritima ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + 5-fluoro-2'-deoxyuridine
-
 Thermotoga maritima DSM 3109 ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + dioxolane thymidine
-
 Thermotoga maritima ADP + dioxolane thymidine 5'-phosphate
-
 ?
2.7.1.21 ATP + thymidine
-
 Thermotoga maritima ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine
-
 Thermotoga maritima DSM 3109 ADP + dTMP
-
 ?
2.7.1.21 additional information no activity with uridine, L-thymidine or 2',3'-dideoxyuridine Thermotoga maritima ?
-
 ?
2.7.1.21 additional information no activity with uridine, L-thymidine or 2',3'-dideoxyuridine Thermotoga maritima DSM 3109 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.21 homotetramer
-
 Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.21 0.29
-
 2',3'-dideoxy-3'-azidothymidine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.29
-
 dioxolane thymidine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.3
-
 thymidine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.33
-
 2',3'-dideoxythymidine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.4
-
 ATP 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.41
-
 2',3'-dideoxy-2',3'-didehydrothymidine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.5
-
 2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.7
-
 2'-deoxyuridine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 0.74
-
 5-fluoro-2'-deoxyuridine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 1.6
-
 2',3'-dideoxy-3'-azidothymidine 82°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 3.5
-
 ATP 82°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 3.5
-
 thymidine 82°C, pH not specified in the publication Thermotoga maritima

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.21 2.5
-
 dioxolane thymidine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 3.8
-
 2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 5.4
-
 2',3'-dideoxy-2',3'-didehydrothymidine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 10
-
 ATP 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 11
-
 2',3'-dideoxythymidine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 18
-
 ATP 82°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 180
-
 2',3'-dideoxy-3'-azidothymidine 82°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 450
-
 2',3'-dideoxy-3'-azidothymidine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 480
-
 2'-deoxyuridine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 600
-
 thymidine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 740
-
 5-fluoro-2'-deoxyuridine 37°C, pH not specified in the publication Thermotoga maritima
2.7.1.21 3500
-
 thymidine 82°C, pH not specified in the publication Thermotoga maritima