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Literature summary extracted from
- Two cycloartenol synthases for phytosterol biosynthesis in Polygala tenuifolia willd (2017), Int. J. Mol. Sci., 18, 2426-2437 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.8 | isozyme CAS1, RNA-sequencing analysis from seedling genome, cloning from cDNA library, DNA and amino acid sequence determination and analysis, isozyme sequence comparison, phylogenetic tree of OSCs, quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Saccharomyces cerevisiae, GC-MS analysis of cycloartenol in yeast | Polygala tenuifolia |
| 5.4.99.8 | isozyme CAS2, RNA-sequencing analysis from seedling genome, cloning from cDNA library, DNA and amino acid sequence determination and analysis, isozyme sequence comparison, phylogenetic tree of OSCs, quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Saccharomyces cerevisiae, GC-MS analysis of cycloartenol in yeast | Polygala tenuifolia |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.8 | (3S)-2,3-epoxy-2,3-dihydrosqualene | Polygala tenuifolia | - |
cycloartenol | - |
r |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.8 | Polygala tenuifolia | A9QW75 | - |
- |
| 5.4.99.8 | Polygala tenuifolia | A9QW77 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.4.99.8 | flower | - |
Polygala tenuifolia | - |
| 5.4.99.8 | additional information | the expression of PtCAS1 is highest in flowers | Polygala tenuifolia | - |
| 5.4.99.8 | additional information | the expression of PtCAS2 is highest in roots | Polygala tenuifolia | - |
| 5.4.99.8 | root | - |
Polygala tenuifolia | - |
| 5.4.99.8 | seedling | - |
Polygala tenuifolia | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.8 | (3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Polygala tenuifolia | cycloartenol | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.8 | OSC | - |
Polygala tenuifolia |
| 5.4.99.8 | PtCAS1 | - |
Polygala tenuifolia |
| 5.4.99.8 | PtCAS2 | - |
Polygala tenuifolia |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 5.4.99.8 | Polygala tenuifolia | the isozyme PtCAS1 is induced 1.5fold by methyl jasmonate. The transcript patterns of the two PtCAS isozyme seedling genes after methyl jasmonate treatment are similar | up |
| 5.4.99.8 | Polygala tenuifolia | the isozyme PtCAS2 is induced 2.0fold by methyl jasmonate. The transcript patterns of the two PtCAS isozyme seedling genes after methyl jasmonate treatment are similar | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.4.99.8 | evolution | the enzyme belongs to the oxidosqualene cyclases (OSCs), enzymes that play a key role in control of the biosynthesis of phytosterols and triterpene saponins | Polygala tenuifolia |
| 5.4.99.8 | physiological function | CAS s play a vital role in sterol biosynthesis, which is essential for plant cell viability | Polygala tenuifolia |
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Release 2023.1 (January 2023)
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