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Literature summary extracted from

  • Yao, L.; Li, H.; Yang, J.; Li, C.; Shen, Y.
    Purification and characterization of a hydroxynitrile lyase from Amygdalus pedunculata Pall (2018), Int. J. Biol. Macromol., 118, 189-194 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.2.10 synthesis Amygdalus pedunculata hydroxynitrile lyase is an excellent biocatalyst and has very high potential for synthesis of enantiopure cyanohydrins Prunus pedunculata

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.10 Ag+ 50 mM, 43% loss of activity Prunus pedunculata
4.1.2.10 Al3+ 50 mM, 26% loss of activity Prunus pedunculata
4.1.2.10 Ba2+ 50 mM, 6% loss of activity Prunus pedunculata
4.1.2.10 Cd2+ 50 mM, 27% loss of activity Prunus pedunculata
4.1.2.10 Cu2+ 50 mM, 23% loss of activity Prunus pedunculata
4.1.2.10 Fe2+ 50 mM, 6% loss of activity Prunus pedunculata
4.1.2.10 Fe3+ 50 mM, 4% loss of activity Prunus pedunculata
4.1.2.10 Hg2+ 50 mM, 54% loss of activity Prunus pedunculata
4.1.2.10 K+ 50 mM, 11% loss of activity Prunus pedunculata
4.1.2.10 Mg2+ 50 mM, 32% loss of activity Prunus pedunculata
4.1.2.10 Mn4+ 50 mM, 39% loss of activity Prunus pedunculata
4.1.2.10 Pb2+ 50 mM, 16% loss of activity Prunus pedunculata
4.1.2.10 Sn2+ 50 mM, 14% loss of activity Prunus pedunculata
4.1.2.10 Sr2+ 50 mM, 13% loss of activity Prunus pedunculata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.10 0.5
-
 (R)-mandelonitrile pH 5.5, 50°C Prunus pedunculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.10 61000
-
 MALDI-TOF mass spectrometry Prunus pedunculata

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Prunus pedunculata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.10
-
 Prunus pedunculata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.10 661
-
 pH 5.5, 50°C Prunus pedunculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 (R)-mandelonitrile
-
 Prunus pedunculata cyanide + benzaldehyde
-
 r
4.1.2.10 cyanide + 3-phenoxybenzaldehyde synthesis of (R)-2-hydroxy-2-(3-phenoxyphenyl)-acetonitrile with 93% enantiomeric excess Prunus pedunculata (R)-2-hydroxy-2-(3-phenoxy-phenyl)-acetonitrile
-
 ?
4.1.2.10 cyanide + benzaldehyde synthesis of (R)-mandelonitrile with 94% enantiomeric excess Prunus pedunculata (R)-mandelonitrile
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.2.10 monomer
-
 Prunus pedunculata

Synonyms

EC Number Synonyms Comment Organism
4.1.2.10 APHNL
-
 Prunus pedunculata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.10 50
-
-
 Prunus pedunculata

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.2.10 10 70 10°C: about 70% of maximal activity, 70°C: about 60% of maximal activity Prunus pedunculata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.10 60
-
 1 h, stable up to Prunus pedunculata
4.1.2.10 70
-
 1 h, 30% loss of activity Prunus pedunculata
4.1.2.10 80
-
 1 h, 70% loss of activity Prunus pedunculata
4.1.2.10 90
-
 1 h, 85% loss of activity Prunus pedunculata
4.1.2.10 100
-
 1 h, complete loss of activity Prunus pedunculata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.10 676.5
-
 (R)-mandelonitrile pH 5.5, 50°C Prunus pedunculata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.10 6
-
-
 Prunus pedunculata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.10 4 8 4.0 pH: about 60% of maximal activity, pH 8.0: about 50% of maximal activity Prunus pedunculata

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.10 4.5
-
 1 h, complete loss of activity Prunus pedunculata
4.1.2.10 5
-
 1 h, 25% loss of activity Prunus pedunculata
4.1.2.10 6 12 1 h, enzyme is stable Prunus pedunculata

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.2.10 1353
-
 (R)-mandelonitrile pH 5.5, 50°C Prunus pedunculata