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Literature summary extracted from

  • Mendes, M.I.; Santos, A.S.; Smith, D.E.; Lino, P.R.; Colaco, H.G.; de Almeida, I.T.; Vicente, J.B.; Salomons, G.S.; Rivera, I.; Blom, H.J.; Leandro, P.
    Insights into the regulatory domain of cystathionine beta-synthase characterization of six variant proteins (2014), Hum. Mutat., 35, 1195-1202 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.22 S-adenosyl-L-methionine half-activation of wild-type at 0.0258 mM, mutant D444N 1.234 mM, mutant S500L 0.063 mM, respectively Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.22 D444N about 95% of wild-type activity, increase in the basal enzymatic activity in presence of pyridoxal 5'-phosphate Homo sapiens
4.2.1.22 K523Sfs?18 enzymatic function of the variants is not impaired, increase in the basal enzymatic activity in presence of pyridoxal 5'-phosphate Homo sapiens
4.2.1.22 L540Q enzymatic function of the variants is not impaired, increase in the basal enzymatic activity in presence of pyridoxal 5'-phosphate Homo sapiens
4.2.1.22 P427L enzymatic function of the variants is not impaired. Mutant lacks activation by S-adenosyl-L-methionine, but binds it at low level and shows an increase in the basal enzymatic activity in presence of pyridoxal 5'-phosphate Homo sapiens
4.2.1.22 S500L about 71% of wild-type activity. Mutant lacks activation by S-adenosyl-L-methionine, but binds it at low level and shows an increase in the basal enzymatic activity in presence of pyridoxal 5'-phosphate Homo sapiens
4.2.1.22 V449G enzymatic function of the variants is not impaired, increase in the basal enzymatic activity in presence of pyridoxal 5'-phosphate Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.22 L-homocysteine
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.22 0.29
-
 L-homocysteine wild-type, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 0.31
-
 L-homocysteine mutant K523Sfs?18, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 0.32
-
 L-homocysteine mutant S500L, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 0.35
-
 L-homocysteine mutant V449G, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 0.4
-
 L-homocysteine mutant D444N, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 0.47
-
 L-homocysteine mutant L540Q, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 0.69
-
 L-homocysteine mutant P427L, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 1.9
-
 L-serine mutant L540Q, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 2
-
 L-serine mutant P427L, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 2.1
-
 L-serine mutant S500L, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 2.7
-
 L-serine mutant V449G, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 2.9
-
 L-serine mutant D444N, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 3.6
-
 L-serine wild-type, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 3.7
-
 L-serine mutant K523Sfs?18, 37°C, pH not specified in the publication Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.22 250000
-
 dynamic light scattering Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.22 Homo sapiens P35520
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.22 L-serine + L-homocysteine
-
 Homo sapiens L-cystathionine + H2O
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.22 3.9
-
 L-homocysteine mutant K523Sfs?18, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 7.7
-
 L-homocysteine mutant P427L, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 12
-
 L-homocysteine mutant V449G, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 12.8
-
 L-homocysteine mutant L540Q, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 12.8
-
 L-homocysteine wild-type, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 13.4
-
 L-homocysteine mutant D444N, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 27.8
-
 L-homocysteine mutant S500L, 37°C, pH not specified in the publication Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.22 0.34
-
 L-serine wild-type, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 0.46
-
 L-serine mutant D444N, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 0.71
-
 L-serine mutant K523Sfs?18, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 0.83
-
 L-serine mutant V449G, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 1.06
-
 L-serine mutant S500L, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 1.93
-
 L-serine mutant P427L, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 2.8
-
 L-homocysteine mutant D444N, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 3
-
 L-homocysteine wild-type, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 3.61
-
 L-serine mutant L540Q, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 4.71
-
 L-homocysteine mutant V449G, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 4.82
-
 L-homocysteine mutant S500L, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 5.22
-
 L-homocysteine mutant K523Sfs?18, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 5.23
-
 L-homocysteine mutant P427L, 37°C, pH not specified in the publication Homo sapiens
4.2.1.22 11
-
 L-homocysteine mutant L540Q, 37°C, pH not specified in the publication Homo sapiens