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Literature summary extracted from

  • Um, Y.; Jin, M.; Lee, D.; Kim, C.; Hong, C.; Lee, Y.; Kim, O.
    Functional characterization of the beta-amyrin synthase gene involved in platycoside biosynthesis in Platycodon grandiflorum (2017), Hortic. Environ. Biotechnol., 58, 613-619 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.39 gene bAS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression in Saccharomyces cerevisiae strain GIL77, real-time RT-PCR enzyme expression analysis, MS analysis metabolite identification Platycodon grandiflorus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.39 (3S)-2,3-epoxy-2,3-dihydrosqualene Platycodon grandiflorus
-
beta-amyrin
-
r

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.39 Platycodon grandiflorus A0A1Z2WUZ5
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.99.39 flower
-
Platycodon grandiflorus
-
5.4.99.39 leaf
-
Platycodon grandiflorus
-
5.4.99.39 additional information about equal enzyme expression in the different tissues, quantitative tissue-specific real-time RT-PCR gene expression analysis Platycodon grandiflorus
-
5.4.99.39 root
-
Platycodon grandiflorus
-
5.4.99.39 stem
-
Platycodon grandiflorus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.39 (3S)-2,3-epoxy-2,3-dihydrosqualene
-
Platycodon grandiflorus beta-amyrin
-
r
5.4.99.39 additional information GC-MS analysis metabolite identification Platycodon grandiflorus ?
-
?

Subunits

EC Number Subunits Comment Organism
5.4.99.39 ? x * 87996, sequence calculation Platycodon grandiflorus

Synonyms

EC Number Synonyms Comment Organism
5.4.99.39 bAS
-
Platycodon grandiflorus
5.4.99.39 PlgOSC1
-
Platycodon grandiflorus

General Information

EC Number General Information Comment Organism
5.4.99.39 evolution PlgOSC1 not only contains a DCTAE motif that is implicated in substrate binding, but also has four repeats of the QXXXGXW motif, which is a typical feature of the triterpene synthase superfamily. PlgOSC1 has a Try residue in the MWCYCR motif that plays a role in the formation of beta-amyrin Platycodon grandiflorus
5.4.99.39 metabolism the enzyme synthesizes beta-amyrin, a central precursor in the platycoside biosynthesis pathway, overview. 69 platycosides are identified from Platycodon grandiflorum Platycodon grandiflorus