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Literature summary extracted from

  • Cobucci-Ponzano, B.; Zorzetti, C.; Strazzulli, A.; Carillo, S.; Bedini, E.; Corsaro, M.; Comfort, D.; Kelly, R.; Rossi, M.; Moracci, M.
    A novel alpha-D-galactosynthase from Thermotoga maritima converts beta-D-galactopyranosyl azide to alpha-galacto-oligosaccharides (2011), Glycobiology, 21, 448-456 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.22 synthesis the mutant is an efficient alpha-galactosynthase producing different galactosylated disaccharides from beta-galactosyl-azide donors and 4-nitrophenyl-alpha-and beta-glycosides as acceptors Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.22 D327G the mutant is an efficient alpha-galactosynthase producing different galactosylated disaccharides from beta-galactosyl-azide donors and 4-nitrophenyl-alpha-and beta-glycosides as acceptors Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.04
-
 4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 65°C, wild-type enzyme Thermotoga maritima
3.2.1.22 0.1
-
 4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 65°C, mutant enzyme D327G Thermotoga maritima
3.2.1.22 0.12
-
 4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 65°C, mutant enzyme D327G, assay performed in 500 mM NaN3 Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Thermotoga maritima G4FEF4
-
-
3.2.1.22 Thermotoga maritima DSM 3109 G4FEF4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Thermotoga maritima 4-nitrophenol + D-galactopyranose
-
 ?
3.2.1.22 4-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Thermotoga maritima DSM 3109 4-nitrophenol + D-galactopyranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 TM1192
-
 Thermotoga maritima
3.2.1.22 TmGalA
-
 Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.22 0.06
-
 4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 65°C, mutant enzyme D327G Thermotoga maritima
3.2.1.22 2.07
-
 4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 65°C, mutant enzyme D327G, assay performed in 500 mM NaN3 Thermotoga maritima
3.2.1.22 65
-
 4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 65°C, wild-type enzyme Thermotoga maritima

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.22 0.6
-
 4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 65°C, mutant enzyme D327G Thermotoga maritima
3.2.1.22 17
-
 4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 65°C, mutant enzyme D327G, assay performed in 500 mM NaN3 Thermotoga maritima
3.2.1.22 1585
-
 4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 65°C, wild-type enzyme Thermotoga maritima