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Literature summary extracted from

  • Motter, F.A.; Kuivanen, J.; Keraenen, H.; Hilditch, S.; Penttilae, M.; Richard, P.
    Categorisation of sugar acid dehydratases in Aspergillus niger (2014), Fungal Genet. Biol., 64, 67-72 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.6 gene dgdA, DNA and amino acid sequence determination and analysis, recombinant expression in Saccharomyces cerevisiae Aspergillus niger
4.2.1.6 gene dgdB, DNA and amino acid sequence determination and analysis, recombinant expression in Saccharomyces cerevisiae Aspergillus niger
4.2.1.68 gene gaaB, DNA and amino acid sequence determination and analysis, recombinant expression in Saccharomyces cerevisiae Aspergillus niger
4.2.1.90 gene lraC, DNA and amino acid sequence determination and analysis, recombinant expression in Saccharomyces cerevisiae Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.6 additional information
-
 additional information Michaelis-Menten kinetics Aspergillus niger
4.2.1.6 8.9
-
 D-galactonate recombinant enzyme, pH 7.0, temperature not specified in the publication Aspergillus niger
4.2.1.6 21.74
-
 D-galactonate recombinant enzyme, pH 7.0, temperature not specified in the publication Aspergillus niger
4.2.1.68 additional information
-
 additional information Michaelis-Menten kinetics Aspergillus niger
4.2.1.68 1.89
-
 L-fuconate recombinant enzyme, pH 7.0, temperature not specified in the publication Aspergillus niger
4.2.1.68 3.92
-
 L-galactonate recombinant enzyme, pH 7.0, temperature not specified in the publication Aspergillus niger
4.2.1.90 additional information
-
 additional information Michaelis-Menten kinetics Aspergillus niger
4.2.1.90 3
-
 L-Rhamnonate recombinant enzyme, pH 7.0, temperature not specified in the publication Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.6 D-galactonate Aspergillus niger
-
 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
4.2.1.6 D-galactonate Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7
-
 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
4.2.1.90 L-rhamnonate Aspergillus niger
-
 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?
4.2.1.90 L-rhamnonate Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7
-
 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.6 Aspergillus niger G3Y8T6
-
-
4.2.1.6 Aspergillus niger G3YE52
-
-
4.2.1.6 Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 G3Y8T6
-
-
4.2.1.6 Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 G3YE52
-
-
4.2.1.68 Aspergillus niger G3Y683
-
-
4.2.1.68 Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 G3Y683
-
-
4.2.1.90 Aspergillus niger G3XPU3
-
-
4.2.1.90 Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 G3XPU3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.6 D-galactonate
-
 Aspergillus niger 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
4.2.1.6 D-galactonate
-
 Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
4.2.1.6 additional information the enzyme only shows activity with D-galactonate. The production of 2-dehydro-3-deoxy sugar is measured with the thiobarbituric acid assay. Substrate specificity analysis, overview. No activity with L-rhamnonate, L-galactonate, D-gulonate, D-gluconate, L-gulonate, D-arabonate, D-lyxonate, L-lyxonate, D-xylonate, mucic acid, L-fuconate, L-mannonate, D-mannonate, D-riborate, L-arabonate, D-galacturonate, and D-glucuronate Aspergillus niger ?
-
 ?
4.2.1.6 additional information the enzyme only shows activity with D-galactonate. The production of 2-dehydro-3-deoxy sugar is measured with the thiobarbituric acid assay. Substrate specificity analysis, overview. No activity with L-rhamnonate, L-galactonate, D-gulonate, D-gluconate, L-gulonate, D-arabonate, D-lyxonate, L-lyxonate, D-xylonate, mucic acid, L-fuconate, L-mannonate, D-mannonate, D-riborate, L-arabonate, D-galacturonate, and D-glucuronate Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 ?
-
 ?
4.2.1.68 D-gluconate
-
 Aspergillus niger ?
-
 ?
4.2.1.68 D-gluconate
-
 Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 ?
-
 ?
4.2.1.68 D-Glucuronate
-
 Aspergillus niger ?
-
 ?
4.2.1.68 D-Glucuronate
-
 Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 ?
-
 ?
4.2.1.68 D-mannonate
-
 Aspergillus niger 2-dehydro-3-deoxy-D-mannonate + H2O
-
 ?
4.2.1.68 L-Fuconate
-
 Aspergillus niger ?
-
 ?
4.2.1.68 L-Fuconate
-
 Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 ?
-
 ?
4.2.1.68 L-fuconate + H2O highly preferred enzyme Aspergillus niger 2-dehydro-3-deoxy-L-fuconate
-
 ?
4.2.1.68 L-galactonate
-
 Aspergillus niger 2-dehydro-3-deoxy-L-galactonate + H2O
-
 ?
4.2.1.68 L-galactonate
-
 Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 2-dehydro-3-deoxy-L-galactonate + H2O
-
 ?
4.2.1.68 L-mannonate
-
 Aspergillus niger 2-dehydro-3-deoxy-L-mannonate + H2O
-
 ?
4.2.1.68 additional information the L-galactonate dehydratase shows highest activity with L-fuconate. The production of 2-dehydro-3-deoxy sugar is measured with the thiobarbituric acid assay. Substrate specificity analysis, overview. No activity with L-rhamnonate, D-gulonate, L-gulonate, D-arabonate, D-lyxonate, L-lyxonate, D-xylonate, mucic acid, D-riborate, L-arabonate, D-galactonate, and D-galacturonate Aspergillus niger ?
-
 ?
4.2.1.68 additional information the L-galactonate dehydratase shows highest activity with L-fuconate. The production of 2-dehydro-3-deoxy sugar is measured with the thiobarbituric acid assay. Substrate specificity analysis, overview. No activity with L-rhamnonate, D-gulonate, L-gulonate, D-arabonate, D-lyxonate, L-lyxonate, D-xylonate, mucic acid, D-riborate, L-arabonate, D-galactonate, and D-galacturonate Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 ?
-
 ?
4.2.1.90 L-mannonate
-
 Aspergillus niger 2-dehydro-3-deoxy-L-mannonate + H2O
-
 ?
4.2.1.90 L-mannonate
-
 Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 2-dehydro-3-deoxy-L-mannonate + H2O
-
 ?
4.2.1.90 L-rhamnonate
-
 Aspergillus niger 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?
4.2.1.90 L-rhamnonate
-
 Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?
4.2.1.90 additional information enzyme LraC shows activity with L-rhamnonate and L-mannonate. The production of 2-dehydro-3-deoxy sugar is measured with the thiobarbituric acid assay. Substrate specificity analysis, overview. No activity with L-galactonate, D-gulonate, D-gluconate, L-gulonate, D-arabonate, D-lyxonate, L-lyxonate, D-xylonate, mucic acid, L-fuconate, D-mannonate, D-riborate, L-arabonate, D-galactonate, D-galacturonate, and D-glucuronate Aspergillus niger ?
-
 ?
4.2.1.90 additional information enzyme LraC shows activity with L-rhamnonate and L-mannonate. The production of 2-dehydro-3-deoxy sugar is measured with the thiobarbituric acid assay. Substrate specificity analysis, overview. No activity with L-galactonate, D-gulonate, D-gluconate, L-gulonate, D-arabonate, D-lyxonate, L-lyxonate, D-xylonate, mucic acid, L-fuconate, D-mannonate, D-riborate, L-arabonate, D-galactonate, D-galacturonate, and D-glucuronate Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.6 D-galactonate dehydratase
-
 Aspergillus niger
4.2.1.6 dgdA
-
 Aspergillus niger
4.2.1.6 dgdB
-
 Aspergillus niger
4.2.1.68 gaaB
-
 Aspergillus niger
4.2.1.90 LraC
-
 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.6 7
-
 assay at Aspergillus niger
4.2.1.68 7
-
 assay at Aspergillus niger
4.2.1.90 7
-
 assay at Aspergillus niger

General Information

EC Number General Information Comment Organism
4.2.1.90 metabolism L-rhamnose is oxidised to L-rhamnonate which is then reacting with a dehydratase. The resulting 3,6-dideoxy-L-erythro-hexulosonic acid (2-dehydro-3-deoxy rhamnonate) is subsequently split by and aldolase to pyruvate and L-lactaldehyde Aspergillus niger