EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.3.15 | gene TPS2, cloning from a cDNA library, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR expression analysis, recombinant expression of His-tagged enzyme without after removing the N-terminal plastid targeting sequence in Escherichia coli strain BL21(DE3) | Artemisia annua |
4.2.3.108 | cloning from a cDNA library, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR expression analysis, recombinant expression of His-tagged enzyme without after removing the N-terminal plastid targeting sequence in Escherichia coli strain BL21(DE3) | Artemisia annua |
4.2.3.116 | AaTPS5, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR expression analysis, recombinant expression of His-tagged enzyme, lacking the N-terminal signal peptide, in Escherichia coli strain BL21(DE3) | Artemisia annua |
4.2.3.117 | AaTPS5, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged enzyme, lacking the N-terminal signal peptide, in Escherichia coli strain BL21(DE3) | Artemisia annua |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.3.15 | additional information | the N-terminal signal peptide of AaTPS2 (46 amino acid residues) before RR motif is truncated and recombinantly expressed in Escherichia coli strain BL21(DE3) | Artemisia annua |
4.2.3.108 | additional information | the N-terminal signal peptide of AaTPS6 (46 amino acid residues) before RR motif is truncated and recombinantly expressed in Escherichia coli strain BL21(DE3) | Artemisia annua |
4.2.3.116 | additional information | the N-terminal signal peptide of AaTPS5 (57 amino acid residues) before RR motif is truncated and recombinantly expressed in Escherichia coli strain BL21(DE3) | Artemisia annua |
4.2.3.117 | additional information | the N-terminal signal peptide of AaTPS5 (57 amino acid residues) before RR motif is truncated and recombinantly expressed in Escherichia coli strain BL21(DE3) | Artemisia annua |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.15 | additional information | - |
additional information | Michaelis-Menten kinetics | Artemisia annua | |
4.2.3.15 | 0.00825 | - |
geranyl diphosphate | pH 7.0, 30°C, recombinant enzyme | Artemisia annua | |
4.2.3.108 | additional information | - |
additional information | Michaelis-Menten kinetics | Artemisia annua | |
4.2.3.108 | 0.0177 | - |
geranyl diphosphate | pH 7.0, 30°C, recombinant enzyme | Artemisia annua | |
4.2.3.116 | 0.01974 | - |
geranyl diphosphate | pH 7.0, 30°C, recombinant enzyme | Artemisia annua | |
4.2.3.117 | 0.01974 | - |
geranyl diphosphate | pH 7.0, 30°C, recombinant enzyme | Artemisia annua |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.3.108 | amyloplast | - |
Artemisia annua | 9501 | - |
4.2.3.108 | chloroplast | - |
Artemisia annua | 9507 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.3.15 | Mg2+ | required, Mg2+ is the highly preferred divalent cation | Artemisia annua | |
4.2.3.15 | Mn2+ | low activity | Artemisia annua | |
4.2.3.15 | additional information | although both Mg2+ and Mn2+ are able to support the catalytic activity of enzyme AaTPS2, an altered product spectrum is observed in the presence of Mn2+ for AaTPS2 | Artemisia annua | |
4.2.3.108 | Mg2+ | required | Artemisia annua | |
4.2.3.108 | Mn2+ | activates | Artemisia annua | |
4.2.3.108 | additional information | the enzyme depends on the presence of divalent cations, Mg2+ is the preferred metal ion | Artemisia annua | |
4.2.3.116 | Mg2+ | required, Mg2+ is the preferred divalent cation | Artemisia annua | |
4.2.3.116 | Mn2+ | low activity | Artemisia annua | |
4.2.3.117 | Mg2+ | required, Mg2+ is the preferred divalent cation | Artemisia annua | |
4.2.3.117 | Mn2+ | low activity | Artemisia annua |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.15 | geranyl diphosphate | Artemisia annua | - |
beta-myrcene + diphosphate | - |
? | |
4.2.3.108 | geranyl diphosphate + H2O | Artemisia annua | - |
1,8-cineole + diphosphate | - |
? | |
4.2.3.116 | geranyl diphosphate | Artemisia annua | stereochemistry is not specified in the publication | camphene + diphosphate | - |
? | |
4.2.3.117 | geranyl diphosphate | Artemisia annua | stereochemistry is not specified in the publication | camphene + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.15 | Artemisia annua | A0A068L9J0 | - |
- |
4.2.3.108 | Artemisia annua | A0A068L889 | - |
- |
4.2.3.116 | Artemisia annua | A0A068L7N8 | - |
- |
4.2.3.117 | Artemisia annua | A0A068L7N8 | cf. EC 4.2.3.116 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.3.15 | recombinant His-tagged enzyme without N-terminal signal peptide from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Artemisia annua |
4.2.3.108 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Artemisia annua |
4.2.3.116 | recombinant His-tagged enzyme without N-terminal signal peptide from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Artemisia annua |
4.2.3.117 | recombinant His-tagged enzyme without N-terminal signal peptide from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Artemisia annua |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.3.15 | leaf | - |
Artemisia annua | - |
4.2.3.15 | additional information | quantitative RT-PCR expression analysis in plants tissues. AaTPS2 exhibits the highest expression level in stem, and low expression in the other tissues | Artemisia annua | - |
4.2.3.15 | root | - |
Artemisia annua | - |
4.2.3.15 | stem | highest expression level of AaTPS2 | Artemisia annua | - |
4.2.3.15 | trichome | glandular | Artemisia annua | - |
4.2.3.108 | inflorescence | - |
Artemisia annua | - |
4.2.3.108 | leaf | high enzyme expression level in young leaves | Artemisia annua | - |
4.2.3.108 | additional information | quantitative RT-PCR expression analysis in plants tissues, AaTPS6 is highly expressed in root and young leaves. All the monoterpene synthase genes show higher expression levels in young leaf than in mature leaf, consistent with the contents of monoterpenes | Artemisia annua | - |
4.2.3.108 | root | highest enzyme expression level | Artemisia annua | - |
4.2.3.108 | stem | - |
Artemisia annua | - |
4.2.3.116 | inflorescence | low enzyme content | Artemisia annua | - |
4.2.3.116 | leaf | transcript of AaTPS5 is more abundant in young leaves compared to immature leaves, no enzyme in mature leaves | Artemisia annua | - |
4.2.3.116 | root | low to moderate enzyme content | Artemisia annua | - |
4.2.3.116 | stem | low enzyme content | Artemisia annua | - |
4.2.3.116 | trichome | glandular | Artemisia annua | - |
4.2.3.117 | inflorescence | low enzyme content | Artemisia annua | - |
4.2.3.117 | leaf | transcriptof AaTPS5 is more abundant in young leaves compared to immature leaves, no enzyme in mature leaves | Artemisia annua | - |
4.2.3.117 | root | low to moderate enzyme content | Artemisia annua | - |
4.2.3.117 | stem | low enzyme content | Artemisia annua | - |
4.2.3.117 | trichome | glandular | Artemisia annua | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.15 | geranyl diphosphate | - |
Artemisia annua | beta-myrcene + diphosphate | - |
? | |
4.2.3.15 | additional information | GC-MS analysis and identification of products from enzyme catalysis. Enzyme AaTPS2 produces a single product, beta-myrcene. No activity with farnesyl diphosphate or geranylgeranyl diphosphate | Artemisia annua | ? | - |
? | |
4.2.3.108 | geranyl diphosphate + H2O | - |
Artemisia annua | 1,8-cineole + diphosphate | - |
? | |
4.2.3.108 | additional information | enzyme AaTPS6 produces multiple products with 1,8-cineole as major product (59.28% of total),in addition to ten other products including sabinene and beta-phellandrene (together 19.04%), alpha-terpineol (7.84%), trans-sabinene hydrate(4.03%), (-)-alpha-pinene (3.00%), cis-beta-terpineol (2.51%), beta-myrcene (2.01%), alpha-thujene (0.69%), as well as two unidentified minor products. No activity with farnesyl diphosphate or geranylgeranyl diphosphate | Artemisia annua | ? | - |
? | |
4.2.3.116 | geranyl diphosphate | stereochemistry is not specified in the publication | Artemisia annua | camphene + diphosphate | - |
? | |
4.2.3.116 | additional information | GC-MS analysis and identification of products from enzyme catalysis, overview. Enzyme AaTPS5 catalyzes the production of five monoterpenes, of which camphene is the major one which accounts for 52.12% of the total, and (-)-alpha-pinene (30.08%), (-)-beta-pinene (3.89%), tricyclene (2.68%), and beta-myrcene (1.23%) are the less abundant products | Artemisia annua | ? | - |
? | |
4.2.3.117 | geranyl diphosphate | stereochemistry is not specified in the publication | Artemisia annua | camphene + diphosphate | - |
? | |
4.2.3.117 | additional information | GC-MS analysis and identification of products from enzyme catalysis, overview. Enzyme AaTPS5 catalyzes the production of five monoterpenes, of which camphene is the major one which accounts for 52.12% of the total, and (-)-alpha-pinene (30.08%), (-)-beta-pinene (3.89%), tricyclene (2.68%), and beta-myrcene (1.23%) are the less abundant products | Artemisia annua | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.3.15 | AaTPS2 | - |
Artemisia annua |
4.2.3.15 | TPS2 | - |
Artemisia annua |
4.2.3.108 | AaTPS6 | - |
Artemisia annua |
4.2.3.108 | TPS6 | - |
Artemisia annua |
4.2.3.116 | AaTPS5 | - |
Artemisia annua |
4.2.3.116 | More | cf. EC 4.2.3.117 | Artemisia annua |
4.2.3.117 | AaTPS5 | - |
Artemisia annua |
4.2.3.117 | More | cf. EC 4.2.3.116 | Artemisia annua |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.3.15 | 35 | - |
- |
Artemisia annua |
4.2.3.108 | 40 | - |
- |
Artemisia annua |
4.2.3.116 | 35 | - |
- |
Artemisia annua |
4.2.3.117 | 35 | - |
- |
Artemisia annua |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.3.108 | 25 | 45 | the recombinant enzyme AaTPS6 activity does not differ greatly at the range from 25°C to 40°C, it reaches peak at 25°C, has its maximal activity at 40°C, and retains 70% at 45°C, activity drops sharply above 40°C | Artemisia annua |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.15 | 0.52 | - |
geranyl diphosphate | pH 7.0, 30°C, recombinant enzyme | Artemisia annua | |
4.2.3.108 | 4.46 | - |
geranyl diphosphate | pH 7.0, 30°C, recombinant enzyme | Artemisia annua | |
4.2.3.116 | 1.49 | - |
geranyl diphosphate | pH 7.0, 30°C, recombinant enzyme | Artemisia annua | |
4.2.3.117 | 1.49 | - |
geranyl diphosphate | pH 7.0, 30°C, recombinant enzyme | Artemisia annua |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.3.15 | 7 | - |
assay at | Artemisia annua |
4.2.3.108 | 7 | - |
assay at | Artemisia annua |
4.2.3.116 | 7 | - |
assay at | Artemisia annua |
4.2.3.117 | 7 | - |
assay at | Artemisia annua |
EC Number | Organism | Comment | Expression |
---|---|---|---|
4.2.3.15 | Artemisia annua | AaTPS2 is upregulated after mechanical wounding, but treatments with phytohormones (salicylic acid, methyl jasmonate, and gibberellin) does not affect the expression of AaTPS2 | up |
4.2.3.108 | Artemisia annua | mechanical wounding induces the expression of all three monoterpene synthase genes, including TPS6, and transcript levels of AaTPS6 are also strongly elevated after treatments with phytohormones, induction by methyl jasmonate (8fold), salicylic acid (4fold), and gibberellin (12fold), suggesting a role of the monoterpene synthase in plant-environment interactions | up |
4.2.3.116 | Artemisia annua | AaTPS5 is upregulated after mechanical wounding and by treatment with 5 mM salicylic acid, 50 mM methyl jasmonate, or 100 mM gibberellin | up |
4.2.3.117 | Artemisia annua | AaTPS5 is upregulated after mechanical wounding and by treatment with 5 mM salicylic acid, 50 mM methyl jasmonate, or 100 mM gibberellin | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.3.15 | evolution | the enzyme AaTPS2 belongs to the TPS superfamily, subclade TPS-b | Artemisia annua |
4.2.3.15 | metabolism | in Artemisia annua, three monoterpene synthases AaTPS2, AaTPS5, and AaTPS6, produce beta-myrcene, camphene, and 1,8-cineole as the major products, respectively, analysis of monoterpenes and sesquiterpenes produced by Artemisia annua plants, overview | Artemisia annua |
4.2.3.108 | evolution | enzyme AaTPS6 belongs to the terpene synthases family, TPS-b subfamily | Artemisia annua |
4.2.3.108 | metabolism | in Artemisia annua, three monoterpene synthases AaTPS2, AaTPS5, and AaTPS6, produce beta-myrcene, camphene, and 1,8-cineole as the major products, respectively | Artemisia annua |
4.2.3.108 | physiological function | the monoterpene synthase TPS6 might play a role in plant-environment interactions | Artemisia annua |
4.2.3.116 | evolution | the enzyme TPS5 belongs to the TPS superfamily, subclade TPS-b | Artemisia annua |
4.2.3.116 | additional information | analysis of monoterpenes and sesquiterpenes produced by Artemisia annua plants, overview | Artemisia annua |
4.2.3.117 | evolution | the enzyme TPS5 belongs to the TPS superfamily, subclade TPS-b | Artemisia annua |
4.2.3.117 | additional information | analysis of monoterpenes and sesquiterpenes produced by Artemisia annua plants, overview | Artemisia annua |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.15 | 63.03 | - |
geranyl diphosphate | pH 7.0, 30°C, recombinant enzyme | Artemisia annua | |
4.2.3.108 | 252 | - |
geranyl diphosphate | pH 7.0, 30°C, recombinant enzyme | Artemisia annua | |
4.2.3.116 | 75.5 | - |
geranyl diphosphate | pH 7.0, 30°C, recombinant enzyme | Artemisia annua | |
4.2.3.117 | 75.5 | - |
geranyl diphosphate | pH 7.0, 30°C, recombinant enzyme | Artemisia annua |