Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bai, W.; Shen, J.; Zhu, Y.; Men, Y.; Sun, Y.; Ma, Y.
    Characteristics and kinetic properties of L-rhamnose isomerase from Bacillus subtilis by isothermal titration calorimetry for the production of D-allose (2015), Food Sci. Technol. Res., 21, 13-22 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.14 expression in Bacillus subtilis Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.14 Ba2+ 1 mM, 68% inhibition Bacillus subtilis
5.3.1.14 Ca2+ 1 mM, 64% inhibition Bacillus subtilis
5.3.1.14 Cu2+ 1 mM, 45% inhibition Bacillus subtilis
5.3.1.14 K+ 1 mM, 68% inhibition Bacillus subtilis
5.3.1.14 Na+ 1 mM, 68% inhibition Bacillus subtilis
5.3.1.14 Na2B4O7 1 mM, 90% inhibition Bacillus subtilis
5.3.1.14 NH4+ 1 mM, 67% inhibition Bacillus subtilis
5.3.1.14 Zn2+ 1 mM, 64% inhibition Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.14 0.49
-
 L-rhamnose pH 8.5, 60°C Bacillus subtilis
5.3.1.14 5.98
-
 D-allose pH 8.5, 60°C Bacillus subtilis
5.3.1.14 8
-
 L-Mannose pH 8.5, 60°C Bacillus subtilis
5.3.1.14 8.64
-
 D-ribose pH 8.5, 60°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.14 Co2+ 1 mM, 10fold activiation Bacillus subtilis
5.3.1.14 Mg2+ 1 mM, 2.9fold activiation Bacillus subtilis
5.3.1.14 Mn2+ 1 mM, 47fold activation Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.14 Bacillus subtilis O05264
-
-
5.3.1.14 Bacillus subtilis 168 O05264
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.14 D-allose
-
 Bacillus subtilis D-psicose
-
 r
5.3.1.14 D-allose
-
 Bacillus subtilis 168 D-psicose
-
 r
5.3.1.14 D-psicose
-
 Bacillus subtilis D-allose
-
 r
5.3.1.14 D-psicose
-
 Bacillus subtilis 168 D-allose
-
 r
5.3.1.14 D-ribose
-
 Bacillus subtilis D-ribulose
-
 r
5.3.1.14 D-ribose
-
 Bacillus subtilis 168 D-ribulose
-
 r
5.3.1.14 L-Mannose
-
 Bacillus subtilis L-Fructose
-
 r
5.3.1.14 L-Mannose
-
 Bacillus subtilis 168 L-Fructose
-
 r
5.3.1.14 L-Rhamnose
-
 Bacillus subtilis L-Rhamnulose
-
 r
5.3.1.14 L-Rhamnose
-
 Bacillus subtilis 168 L-Rhamnulose
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.1.14 ? x * 48000, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.14 70
-
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.14 60
-
 4 h, 85% residual activity Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.14 0.74
-
 D-allose pH 8.5, 60°C Bacillus subtilis
5.3.1.14 7.7
-
 L-rhamnose pH 8.5, 60°C Bacillus subtilis
5.3.1.14 10.5
-
 L-Mannose pH 8.5, 60°C Bacillus subtilis
5.3.1.14 30.7
-
 D-ribose pH 8.5, 60°C Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.14 0.12
-
 D-allose pH 8.5, 60°C Bacillus subtilis
5.3.1.14 1.3
-
 L-Mannose pH 8.5, 60°C Bacillus subtilis
5.3.1.14 3.6
-
 D-ribose pH 8.5, 60°C Bacillus subtilis
5.3.1.14 15.75
-
 L-rhamnose pH 8.5, 60°C Bacillus subtilis