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Literature summary extracted from

  • Sun, R.; Liu, S.; Tang, Z.Z.; Zheng, T.R.; Wang, T.; Chen, H.; Li, C.L.; Wu, Q.
    beta-Amyrin synthase from Conyza blinii expressed in Saccharomyces cerevisiae (2017), FEBS open bio, 7, 1575-1585 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.39 gene BAS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional recombinant expressison in Saccharomyces cerevisiae strain INVSc1 under the control of GAL1 promoter yielding beta-amyrin, beta-amyrin biosynthesis pathway engineered in yeast, overview. Metabolite identification by GC-MS/MS analysis Eschenbachia blinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.39 (3S)-2,3-epoxy-2,3-dihydrosqualene Eschenbachia blinii
-
beta-amyrin
-
r

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.39 Eschenbachia blinii A0A2P0VE73 i.e. Conyza blinii, collected in 2014 from Panzhihua, Sichuan, China
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.99.39 seedling
-
Eschenbachia blinii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.39 (3S)-2,3-epoxy-2,3-dihydrosqualene
-
Eschenbachia blinii beta-amyrin
-
r
5.4.99.39 additional information metabolite identification by GC-MS/MS analysis Eschenbachia blinii ?
-
?

Subunits

EC Number Subunits Comment Organism
5.4.99.39 ? x * 87700, about, sequence calculation Eschenbachia blinii

Synonyms

EC Number Synonyms Comment Organism
5.4.99.39 bAS
-
Eschenbachia blinii
5.4.99.39 betaAS
-
Eschenbachia blinii
5.4.99.39 CbbAS
-
Eschenbachia blinii
5.4.99.39 OSC
-
Eschenbachia blinii
5.4.99.39 Oxidosqualene cyclase
-
Eschenbachia blinii

Expression

EC Number Organism Comment Expression
5.4.99.39 Eschenbachia blinii the transcription of gene CbbAS is upregulated 4-24 h after treatment of seedlings with methyl jasmonate up

General Information

EC Number General Information Comment Organism
5.4.99.39 evolution the sequence of the mature protein contains the highly conserved motifs (QXXXGXW/DCTAE) of OSCs and (MWCYCR) of beta-amyrin synthases Eschenbachia blinii
5.4.99.39 physiological function Conyza blinii is a widely used medicinal herb in southwestern China. The main pharmacological components of Conyza blinii are a class of oleanane-type pentacyclic triterpene glycosides, known as conyzasaponins, which are thought to be synthesized from beta-amyrin. The beta-amyrin synthase mediates cyclization of 2,3-oxidosqualene to yield beta-amyrin. The enzyme is an oxidosqualene cyclase (OSC) involved in conyzasaponin biosynthesis Eschenbachia blinii