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Literature summary extracted from

  • Baas, B.J.; Zandvoort, E.; Wasiel, A.A.; Poelarends, G.J.
    Demethionylation of Pro-1 variants of 4-oxalocrotonate tautomerase in Escherichia coli by co-expression with an engineered methionine aminopeptidase (2014), FEBS Open Bio, 4, 651-658 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.2.6 expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.2.6 0.05
-
 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate at pH 7.3 and 22°C Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.2.6 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate Pseudomonas putida
-
 (3E)-2-oxohex-3-enedioate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.2.6 Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.2.6 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate
-
 Pseudomonas putida (3E)-2-oxohex-3-enedioate
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.2.6 ? x * 6812, electrospray ionization mass spectrometry Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
5.3.2.6 4-OT
-
 Pseudomonas putida
5.3.2.6 4-oxalocrotonate tautomerase
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.2.6 1330
-
 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate at pH 7.3 and 22°C Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.2.6 26600
-
 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate at pH 7.3 and 22°C Pseudomonas putida