EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.47 | to 2.2 A resolution | Bacillus anthracis |
4.2.1.22 | to 2.2 A resolution | Bacillus anthracis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.47 | A72S | mutant produces more H2S than wild-type | Bacillus anthracis |
2.5.1.47 | E220R | not able to release H2S | Bacillus anthracis |
4.2.1.22 | A72S | mutant produces more H2S than wild-type | Bacillus anthracis |
4.2.1.22 | E220R | not able to release H2S | Bacillus anthracis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.47 | 1.168 | - |
O-acetyl-L-serine | wild-type, pH 8.2, 37°C | Bacillus anthracis | |
2.5.1.47 | 1.65 | - |
O-acetyl-L-serine | mutant A72S, pH 8.2, 37°C | Bacillus anthracis | |
2.5.1.47 | 2.05 | - |
O-acetyl-L-serine | mutant E220R, pH 8.2, 37°C | Bacillus anthracis | |
2.5.1.47 | 6.18 | - |
O-acetyl-L-serine | production of H2S, wild-type, pH 8.2, 37°C | Bacillus anthracis | |
2.5.1.47 | 9.01 | - |
O-acetyl-L-serine | production of H2S, mutant A72S, pH 8.2, 37°C | Bacillus anthracis | |
4.2.1.22 | 0.32 | - |
L-homocysteine | wild-type, production of H2S, pH 8.2, 37°C | Bacillus anthracis | |
4.2.1.22 | 1.51 | - |
L-homocysteine | mutant E220R, pH 8.2, 37°C | Bacillus anthracis | |
4.2.1.22 | 2.73 | - |
L-homocysteine | wild-type, pH 8.2, 37°C | Bacillus anthracis | |
4.2.1.22 | 3.59 | - |
L-homocysteine | mutant A72S, pH 8.2, 37°C | Bacillus anthracis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.47 | Bacillus anthracis | A0A1J9VES8 | enzyme shows activities of both EC 4.2.1.22 and 2.5.1.47 | - |
4.2.1.22 | Bacillus anthracis | A0A1J9VES8 | enzyme shows activities of both EC 4.2.1.22 and 2.5.1.47 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.47 | L-cysteine + H2O | - |
Bacillus anthracis | L-serine + H2S | - |
? | |
2.5.1.47 | L-cysteine + L-homocysteine | - |
Bacillus anthracis | L-cystathionine + H2S | - |
? | |
2.5.1.47 | additional information | no substrate: L-serine | Bacillus anthracis | ? | - |
? | |
2.5.1.47 | O-acetyl-L-serine | - |
Bacillus anthracis | L-cysteine + acetate | - |
? | |
2.5.1.47 | O-acetyl-L-serine + H2S | - |
Bacillus anthracis | L-cysteine + acetic acid | - |
? | |
4.2.1.22 | L-cysteine + H2O | - |
Bacillus anthracis | L-serine + H2S | - |
? | |
4.2.1.22 | L-cysteine + L-homocysteine | - |
Bacillus anthracis | L-cystathionine + H2S | - |
? | |
4.2.1.22 | additional information | no substrate: L-serine | Bacillus anthracis | ? | - |
? | |
4.2.1.22 | O-acetyl-L-serine | - |
Bacillus anthracis | L-cysteine + acetate | - |
? | |
4.2.1.22 | O-acetyl-L-serine + H2S | - |
Bacillus anthracis | L-cysteine + acetic acid | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.47 | ? | x * 31400, SDS-PAGE | Bacillus anthracis |
4.2.1.22 | ? | x * 31400, SDS-PAGE | Bacillus anthracis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.47 | 0.7 | - |
O-acetyl-L-serine | production of H2S, wild-type, pH 8.2, 37°C | Bacillus anthracis | |
2.5.1.47 | 0.9 | - |
O-acetyl-L-serine | production of H2S, mutant A72S, pH 8.2, 37°C | Bacillus anthracis | |
2.5.1.47 | 4.46 | - |
O-acetyl-L-serine | wild-type, pH 8.2, 37°C | Bacillus anthracis | |
2.5.1.47 | 4.88 | - |
O-acetyl-L-serine | mutant A72S, pH 8.2, 37°C | Bacillus anthracis | |
2.5.1.47 | 5.46 | - |
O-acetyl-L-serine | mutant E220R, pH 8.2, 37°C | Bacillus anthracis | |
4.2.1.22 | 3.38 | - |
L-homocysteine | wild-type, production of H2S, pH 8.2, 37°C | Bacillus anthracis | |
4.2.1.22 | 5.74 | - |
L-homocysteine | mutant E220R, pH 8.2, 37°C | Bacillus anthracis | |
4.2.1.22 | 8.39 | - |
L-homocysteine | wild-type, pH 8.2, 37°C | Bacillus anthracis | |
4.2.1.22 | 10.91 | - |
L-homocysteine | mutant A72S, pH 8.2, 37°C | Bacillus anthracis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.47 | pyridoxal 5'-phosphate | cofactor is highly flexible due to the active site being wide open | Bacillus anthracis | |
4.2.1.22 | pyridoxal 5'-phosphate | cofactor is highly flexible due to the active site being wide open | Bacillus anthracis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.47 | 0.1 | - |
O-acetyl-L-serine | production of H2S, mutant A72S, pH 8.2, 37°C | Bacillus anthracis | |
2.5.1.47 | 0.11 | - |
O-acetyl-L-serine | production of H2S, wild-type, pH 8.2, 37°C | Bacillus anthracis | |
2.5.1.47 | 2.67 | - |
O-acetyl-L-serine | mutant E220R, pH 8.2, 37°C | Bacillus anthracis | |
2.5.1.47 | 2.96 | - |
O-acetyl-L-serine | mutant A72S, pH 8.2, 37°C | Bacillus anthracis | |
2.5.1.47 | 3.82 | - |
O-acetyl-L-serine | wild-type, pH 8.2, 37°C | Bacillus anthracis | |
2.5.1.47 | 10.56 | - |
L-homocysteine | wild-type, production of H2S, pH 8.2, 37°C | Bacillus anthracis | |
4.2.1.22 | 3.04 | - |
L-homocysteine | mutant A72S, pH 8.2, 37°C | Bacillus anthracis | |
4.2.1.22 | 3.06 | - |
L-homocysteine | wild-type, pH 8.2, 37°C | Bacillus anthracis | |
4.2.1.22 | 3.83 | - |
L-homocysteine | mutant E220R, pH 8.2, 37°C | Bacillus anthracis | |
4.2.1.22 | 10.56 | - |
L-homocysteine | wild-type, production of H2S, pH 8.2, 37°C | Bacillus anthracis |